Journal ArticleDOI
The temperature dependence in the hydraulic conductivity, Lp, of the mouse sperm plasma membrane shows a discontinuity between 4 and 0 degrees C.
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TLDR
Examination of mouse sperm in hyposmotic media by microscopy revealed little swelling of the cells, indicating that the low-amplitude swelling model may be the one more applicable to these cells, consistent with the observed marked increase in Osmcrit and decrease in t at 0 degrees C compared to the other temperatures.About:
This article is published in Cryobiology.The article was published on 1995-06-01. It has received 58 citations till now. The article focuses on the topics: Sperm plasma membrane & Genetic model.read more
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Journal ArticleDOI
Semen cryopreservation in domestic animals: a damaging and capacitating phenomenon.
TL;DR: Successful semen cryopreservation enhances the advantages of AI over natural breeding, as long-term storage facilitates semen transport over distances, permits the quarantine of semen, and enables extended use of superior germplasm, even after the sire's death.
Journal ArticleDOI
Comparison of Glycerol, Other Polyols, Trehalose, and Raffinose to Provide a Defined Cryoprotectant Medium for Mouse Sperm Cryopreservation☆
TL;DR: The glycerol/trehalose combination provides a defined cryoprotectant which, when used with addition by dialysis prefreeze and direct insemination postthaw, yields a satisfactory yield of fertilized eggs in an in vitro fertilization system.
Book ChapterDOI
Fundamental Cryobiology of Mammalian Spermatozoa
TL;DR: This chapter discusses the fundamental cryobiology of mammalian spermatozoa and the optimization of CPA addition and removal procedures.
Journal ArticleDOI
Cryopreservation of Mouse Spermatozoa
Nucharin Songsasen,S.P. Leibo +1 more
TL;DR: Mouse spermatozoa cryopreserved by the method described here are capable of fertilizing oocytes at a rather high rate and embryo production by IVF seemed to be improved using spermatozosa frozen after being seeded.
Journal ArticleDOI
Osmotic tolerance limits and properties of murine spermatozoa.
Cara E. Willoughby,Cara E. Willoughby,Peter Mazur,A.T. Peter,John K. Critser,John K. Critser +5 more
TL;DR: The data indicate that motility is substantially more sensitive to osmotic stress than either mitochondrial viability or membrane integrity and that mouse spermatozoa should be maintained within 76-124% of their isosmotic volume during cryopreservation in order to maintain > 80% of pretreatment motility.