Journal ArticleDOI
THE THREE DS OF PCR-BASED GENOMIC ANALYSIS OF PHYTOBACTERIA: Diversity, Detection, and Disease Diagnosis
Reads0
Chats0
TLDR
Genetic diversity maps provide a framework to understand the taxonomy, population structure, and dynamics of phytobacteria and provide a high-resolution framework to devise sensitive, specific, and rapid methods for pathogen detection, plant disease diagnosis, as well as management of disease risk.Abstract:
▪ Abstract The advent of molecular biology in general and the polymerase chain reaction in particular have greatly facilitated genomic analyses of microorganisms, provide enhanced capability to characterize and classify strains, and facilitate research to assess the genetic diversity of populations. The diversity of large populations can be assessed in a relatively efficient manner using rep-PCR-, AFLP-, and AP-PCR/RAPD-based genomic fingerprinting methods, especially when combined with computer-assisted pattern analysis. Genetic diversity maps provide a framework to understand the taxonomy, population structure, and dynamics of phytobacteria and provide a high-resolution framework to devise sensitive, specific, and rapid methods for pathogen detection, plant disease diagnosis, as well as management of disease risk. A variety of PCR-based fingerprinting protocols such as rDNA-based PCR, ITS-PCR, ARDRA, T-RFLPs, and tRNA-PCR have been devised, and numerous innovative approaches using specific primers have ...read more
Citations
More filters
Journal ArticleDOI
Advanced methods of plant disease detection. A review
Federico Martinelli,Riccardo Scalenghe,Salvatore Davino,Stefano Panno,Giuseppe Scuderi,Paolo Ruisi,Paolo Villa,Daniela Stroppiana,Mirco Boschetti,Luiz Ricardo Goulart,Cristina E. Davis,Abhaya M. Dandekar +11 more
TL;DR: Modern methods based on nucleic acid and protein analysis are described, which represent unprecedented tools to render agriculture more sustainable and safe, avoiding expensive use of pesticides in crop protection.
Journal ArticleDOI
Xanthomonas axonopodis pv. citri: factors affecting successful eradication of citrus canker.
TL;DR: Citrus canker is not present in all subtropical to tropical regions of citriculture in the world, so considerable regulatory efforts are expended to prevent the introduction and spread of X. axonopodis pv.
Journal ArticleDOI
Citrus Canker: The Pathogen and Its Impact
TL;DR: The current USDA system for protecting agricultural industries has been overwhelmed and has sometimes failed to intercept a number of introductions of exotic pests, including plant pathogens.
Journal ArticleDOI
Innovative tools for detection of plant pathogenic viruses and bacteria
María M. López,Edson Bertolini,Antonio Olmos,Paola Caruso,María Teresa Gorris,Pablo Llop,Ramón Penyalver,Mariano Cambra +7 more
TL;DR: The advances in research that will result from the sequencing of many plant pathogen genomes represent a new source of information for the future development of sensitive and specific detection techniques for these microorganisms.
Journal ArticleDOI
Development, registration and commercialization of microbial pesticides for plant protection
TL;DR: The future of microbial pesticides is not only in developing new active ingredients based on microorganisms beneficial to plants, but in producing self-protected plants by transforming agronomically high-value crop plants with genes from biological control agents.
References
More filters
Journal ArticleDOI
DNA polymorphisms amplified by arbitrary primers are useful as genetic markers
TL;DR: A new DNA polymorphism assay based on the amplification of random DNA segments with single primers of arbitrary nucleotide sequence is described, suggesting that these polymorphisms be called RAPD markers, after Random Amplified Polymorphic DNA.
Journal ArticleDOI
AFLP: a new technique for DNA fingerprinting.
Pieter Vos,René Cornelis Josephus Hogers,Marjo Bleeker,Martin Reijans,Theo van de Lee,Miranda Hornes,Adrie Friters,Jerina Pot,Johan Paleman,Martin Kuiper,Marc Zabeau +10 more
TL;DR: The AFLP technique provides a novel and very powerful DNA fingerprinting technique for DNAs of any origin or complexity that allows the specific co-amplification of high numbers of restriction fragments.
Journal ArticleDOI
16S ribosomal DNA amplification for phylogenetic study.
TL;DR: A set of oligonucleotide primers capable of initiating enzymatic amplification (polymerase chain reaction) on a phylogenetically and taxonomically wide range of bacteria is described in this paper.
Journal ArticleDOI
Report of the Ad Hoc Committee on Reconciliation of Approaches to Bacterial Systematics
Lawrence G. Wayne,Don J. Brenner,R. R. Colwell,Patrick A. D. Grimont,O. Kandler,Micah I. Krichevsky,L. H. Moore,W. E. C. Moore,R. G. E. Murray,Erko Stackebrandt,M. P. Starr,H. G. Truper +11 more
Journal ArticleDOI
Fingerprinting genomes using PCR with arbitrary primers
John Welsh,Michael McClelland +1 more
TL;DR: The generality of the arbitrarily primed PCR method is demonstrated by application to twenty four strains from five species of Staphylococcus, eleven strains of Streptococcus pyogenes and three varieties of Oryza sativa.