Journal ArticleDOI
The use of fluorescein isothiocyanate in the determination of the bacterial biomass of grassland soil.
Lorne A. Babiuk,Eldor A. Paul +1 more
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The number of bacteria decreased in a linear fashion with depth but no relation was found between the numbers obtained by direct microscopy and those by plate counting, but each of the methods showed a high relationship between the size of the bacterial population present at each depth on the differe...Abstract:
Quantitative recoveries of bacteria added to a number of soils were obtained using fluorescein isothiocyanate (FITC) as a stain. Enzyme fluorescence, using fluorescein diacetate or dibutyrate, coul...read more
Citations
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Journal ArticleDOI
A physiological method for the quantitative measurement of microbial biomass in soils
TL;DR: The respiratory method provides reproducible estimates of biomass size within 1–3 h after soil amendment, and can be combined without difficulty with a selective inhibition method for determination of bacterial and fungal contributions to soil metabolism.
Journal ArticleDOI
Use of fluorochromes for direct enumeration of total bacteria in environmental samples: past and present.
R L Kepner,J R Pratt +1 more
TL;DR: A review of the use of 3,6-bis[dimethylamino]acridinium chloride (acridine orange) and 4',6-diamidino-2-phenylindole (DAPI) stain for direct enumeration of bacteria is presented in this paper.
Journal ArticleDOI
Elevated atmospheric CO2 and feedback between carbon and nitrogen cycles
Donald R. Zak,Kurt S. Pregitzer,Peter S. Curtis,James A. Teeri,Robert Fogel,Diana L. Randlett +5 more
TL;DR: In this paper, a conceptual model describing the influence of elevated atmospheric CO2 on plant production, soil microorganisms, and the cycling of C and N in the plant-soil system was proposed.
Journal ArticleDOI
Litter placement effects on microbial and organic matter dynamics in an agroecosystem
TL;DR: The surface placement of straw in no-till agriculture allowed management of microclimate and microbial populations so that losses of soil organic matter and nutrients were minimized and the increase in the ratio of fungal to bacterial activity was reduced.
Journal ArticleDOI
Microbial and Faunal Interactions and Effects on Litter Nitrogen and Decomposition in Agroecosystems
Michael H. Beare,Robert W. Parmelee,Paul F. Hendrix,Weixin Cheng,David C. Coleman,D. A. Crossley +5 more
TL;DR: The results suggest that litter placement can strongly influence the com- position of decomposer communities and that the resulting trophic relationships are important to determining the rates and timing of plant litter decomposition and N dynamics.
References
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Journal ArticleDOI
The maintenance requirement of escherichia coli
TL;DR: The definition of energy of maintenance proposed by McGrew and Mallette will be modified such that the definition coincides with an experimentally measurable quantity, the specific maintenance (see equation 2).
Journal ArticleDOI
Fluorescent-Antibody Approach to Study of Rhizobia in Soil
TL;DR: Application of fluorescent-antibody (FA) techniques to the study of rhizobia as free-living soil bacteria was explored and Antiserum to a particular strain of Rhizobium japonicum proved specific in both agglutination and FA tests.
Journal ArticleDOI
Autoradiography as a tool in microbial ecology.
Thomas D. Brock,M. Louise Brock +1 more
TL;DR: It is clear that the behaviour of the pure culture reveals the potentiality of the organism in Nature, but does not show what the organism was actually doing, since the organism has undoubtedly adapted to the cultural conditions used.
Journal ArticleDOI
Nitrogen fixation in grassland and associated cultivated ecosystems
TL;DR: In this article, Nitrogen fixation in the natural, Agropyron-Koeleria grassland ecosystem was studied using the C2H2-C2H4 and N15 assays.
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