Thermotoga lettingae sp. nov., a novel thermophilic, methanol-degrading bacterium isolated from a thermophilic anaerobic reactor.
TLDR
Strain TMOT was able to degrade methanol to CO2 and H2 in syntrophic culture with Methanothermobacter thermautotrophicus AH or Thermodesulfovibrio yellowstonii and is related to Thermotoga subterranea andThermotoga elfii.Abstract:
A novel, anaerobic, non-spore-forming, mobile, Gram-negative, thermophilic bacterium, strain TMOT, was isolated from a thermophilic sulfate-reducing bioreactor operated at 65 C with methanol as the sole substrate. The G+C content of the DNA of strain TMOT was 39.2 mol%. The optimum pH, NaCl concentration, and temperature for growth were 7.0, 1.0%, and 65 degrees C, respectively. Strain TMOT was able to degrade methanol to CO2 and H2 in syntrophic culture with Methanothermobacter thermautotrophicus AH or Thermodesulfovibrio yellowstonii. Thiosulfate, elemental sulfur, Fe(III) and anthraquinone-2,6-disulfonate were able to serve as electron acceptors during methanol degradation. In the presence of thiosulfate or elemental sulfur, methanol was converted to CO2 and partly to alanine. In pure culture, strain TMOT was also able to ferment methanol to acetate, CO2 and H2. However, this degradation occurred slower than in syntrophic cultures or in the presence of electron acceptors. Yeast extract was required for growth. Besides growing on methanol, strain TMOT grew by fermentation on a variety of carbohydrates including monomeric and oligomeric sugars, starch and xylan. Acetate, alanine, CO2, H2, and traces of ethanol, lactate and alpha-aminobutyrate were produced during glucose fermentation. Comparison of 16S rDNA genes revealed that strain TMOT is related to Thermotoga subterranea (98%) and Thermotoga elfii (98%). The type strain is TMOT (= DSM 14385T = ATCC BAA-301T). On the basis of the fact that these organisms differ physiologically from strain TMOT, it is proposed that strain TMOT be classified as a new species, within the genus Thermotoga, as Thermotoga lettingae.read more
Citations
More filters
Journal ArticleDOI
Predominant archaea in marine sediments degrade detrital proteins
Karen G. Lloyd,Lars Schreiber,Dorthe Groth Petersen,Kasper Urup Kjeldsen,Mark A. Lever,Andrew D. Steen,Ramunas Stepanauskas,Michael Richter,Sara Kleindienst,Sabine Lenk,Andreas Schramm,Bo Barker Jørgensen +11 more
TL;DR: It is shown that the uncultured miscellaneous crenarchaeotal group (MCG) and marine benthic group-D (MBG-D) are among the most numerous archaea in the marine sub-sea floor and may have a previously undiscovered role in protein remineralization in anoxic marine sediments.
Journal ArticleDOI
Syntrophic Acetate-Oxidizing Microbes in Methanogenic Environments
TL;DR: Surprisingly, some of these bacteria can also axenically grow on hydrogen/CO(2) to produce acetate, which means that the bacteria can utilize both substrates and products reversibly.
Journal ArticleDOI
Microbial characteristics in anaerobic digestion process of food waste for methane production–A review
TL;DR: A critical summary of microbial characteristics was provided to obtain connects of microbial community structure with operational conditions at various states of AD, such as mesophilic and thermophilic, wet and dry, success and failure, pretreated or not, lab-scale and full-scale.
Journal ArticleDOI
Microbial dark matter ecogenomics reveals complex synergistic networks in a methanogenic bioreactor.
Masaru K. Nobu,Takashi Narihiro,Christian Rinke,Yoichi Kamagata,Susannah G. Tringe,Tanja Woyke,Wen Tso Liu +6 more
TL;DR: State-of-the-art omics tools are employed to generate draft genomes and transcriptomes for uncultivated organisms spanning 15 phyla and the first genomic insight into candidate phyla Atribacteria, Hydrogenedentes and Marinimicrobia in methanogenic environments is reported.
Journal ArticleDOI
Extremely thermophilic microorganisms for biomass conversion: status and prospects.
Sara E. Blumer-Schuette,Irina Kataeva,Irina Kataeva,Janet Westpheling,Janet Westpheling,Michael W. W. Adams,Michael W. W. Adams,Robert M. Kelly,Robert M. Kelly +8 more
TL;DR: Characterization of extremely thermophilic enzymes implicated in the deconstruction of lignocellulose can be identified in genome sequences, and many more promising biocatalysts probably remain annotated as 'hypothetical proteins'.
References
More filters
Journal ArticleDOI
A procedure for the isolation of deoxyribonucleic acid from micro-organisms
TL;DR: A method has been described for the isolation of DNA from micro-organisms which yields stable, biologically active, highly polymerized preparations relatively free from protein and RNA, and Representative samples have been characterized for their thermal stability and sedimentation behaviour.
Journal ArticleDOI
ARB: a software environment for sequence data
Wolfgang Ludwig,Oliver Strunk,Ralf Westram,Lothar Richter,Harald Meier,Yadhukumar,Arno Buchner,Tina Lai,Susanne Steppi,Gangolf Jobb,Wolfram Förster,Igor Brettske,Stefan Gerber,Anton W. Ginhart,Oliver Gross,Silke Grumann,Stefan Hermann,Ralf Jost,Andreas König,Thomas Liss,Ralph Lüßmann,Michael May,Björn Nonhoff,Boris Reichel,Robert Strehlow,Alexandros Stamatakis,Norbert Stuckmann,Alexander Vilbig,Michael Lenke,Thomas Ludwig,Arndt Bode,Karl-Heinz Schleifer +31 more
TL;DR: The ARB program package comprises a variety of directly interacting software tools for sequence database maintenance and analysis which are controlled by a common graphical user interface.
Journal ArticleDOI
The quantitative measurement of DNA hybridization from renaturation rates.
TL;DR: A new method is proposed to measure relatedness amongst bacteria, based on renaturation rate determinations of DNA types and their mixture, and the advantages are summarized.
Book ChapterDOI
[100] A procedure for the isolation of deoxyribonucleic acid from microorganisms
Journal ArticleDOI
Sequence heterogeneities of genes encoding 16S rRNAs in Paenibacillus polymyxa detected by temperature gradient gel electrophoresis.
Ulrich Nübel,Bert Engelen,A Felske,J Snaidr,A Wieshuber,Rudolf Amann,Wolfgang Ludwig,H. Backhaus +7 more
TL;DR: The results demonstrate the usefulness of TGGE for the structural analysis of heterogeneous rRNA genes together with their expression, stress problems of the generation of meaningful data for 16S rRNA sequences and probe designs, and might have consequences for evolutionary concepts.