Three-dimensional structure determination by electron microscopy of two-dimensional crystals
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This article is published in Progress in Biophysics & Molecular Biology.The article was published on 1982-01-01 and is currently open access. It has received 493 citations till now. The article focuses on the topics: Scanning confocal electron microscopy & Energy filtered transmission electron microscopy.read more
Citations
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EMAN: semiautomated software for high-resolution single-particle reconstructions.
TL;DR: EMAN (Electron Micrograph ANalysis), a software package for performing semiautomated single-particle reconstructions from transmission electron micrographs, was written from scratch in C++ and is provided free of charge on the Web site.
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Cryo-electron microscopy of vitrified specimens.
Jacques Dubochet,Marc Adrian,J.-J. Chang,Jean-Claude Homo,J. Lepault,Alasdair W. McDowall,Patrick Schultz +6 more
TL;DR: Water is the most abundant component of biological material, but it is systematically excluded from conventional electron microscopy, because water evaporates rapidly under the vacuum conditions of an electron microscope.
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Refined structure of alpha beta-tubulin at 3.5 A resolution.
TL;DR: In this paper, a refined model of the alpha-beta-tubulin dimer was presented, which includes residues alpha: 2-34, alpha:61-439, beta:2-437, one molecule of GTP, one of GDP, and one of taxol, as well as one magnesium ion near the M-loop in the alpha subunit.
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Structure of purple membrane from halobacterium halobium: recording, measurement and evaluation of electron micrographs at 3.5 Å resolution
TL;DR: In this article, the strength of the very weak high-resolution Fourier components of the image of a two-dimensional crystal was determined using real space correlation analysis, and the amplitude and phase information was extracted from the distortion-corrected image of the crystal.
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Structure and function of aquaporin water channels
Alan S. Verkman,Alok K. Mitra +1 more
TL;DR: Evidence for possible movement of ions and carbon dioxide through the aquaporins is reviewed here, as well as evidence for direct regulation of aquaporin function by posttranslational modification such as phosphorylation.
References
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Three-dimensional model of purple membrane obtained by electron microscopy
Richard Henderson,P. N. T. Unwin +1 more
TL;DR: A 7-Å resolution map of the purple membrane has been obtained by electron microscopy of tilted, unstained specimens and shows that Lipid bilayer regions fill the spaces between the protein molecules.
Journal ArticleDOI
A negative staining method for high resolution electron microscopy of viruses.
S. Brenner,R.W. Horne +1 more
TL;DR: A simple technique has been developed for the study of the external form and structure of virus particles by mixing virus preparations with 1% phosphotungstic acid adjusted to pH 7.5 and spraying directly onto electron microscope supporting films made from evaporated carbon.
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Reconstruction of Three Dimensional Structures from Electron Micrographs
D. J. De Rosier,Aaron Klug +1 more
TL;DR: General principles are formulated for the objective reconstruction of a three dimensional object from a set of electron microscope images and applied to the calculation of aThree dimensional density map of the tail of bacteriophage T4.
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Molecular structure determination by electron microscopy of unstained crystalline specimens.
P. N. T. Unwin,Richard Henderson +1 more
TL;DR: The projected structures of two unstained periodic biological specimens, the purple membrane and catalase, have been determined by electron microscopy to resolutions of 7 A and 9 A, respectively.
Journal ArticleDOI
The Reconstruction of a Three-Dimensional Structure from Projections and its Application to Electron Microscopy
TL;DR: In this paper, the Fourier transform is used to combine data from a number of different views of a transmission electron micrograph to reconstruct a 3D image of a given particle to a given degree of resolution.
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Three-dimensional model of purple membrane obtained by electron microscopy
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