Journal ArticleDOI
Trophectoderm biopsy in human blastocysts
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The results demonstrate the feasibility of trophectoderm biopsy in human blastocysts and that sufficient extra-embryonic material can be obtained by this technique for preimplantation diagnosis of genetic disorders.Abstract:
Trophectoderm biopsy was carried out on 47 human blastocysts. A slit was made in the zona pellucida opposite the inner cell mass by micromanipulative techniques. The human blastocyst zona offered more resistance to slitting compared to that of the mouse. After 18-24 h, controlled herniation of the trophectoderm cells was observed. These cells were biopsied when the diameter of the herniation was approximately equal to that of the blastocyst. The size of the slit and the stage of embryonic development at which slitting was performed were important for successful herniation to occur. After slitting, 76% of day 5-6 blastocysts showed herniation whilst only 42% of day 7-8 blastocysts herniated. Further development of the manipulated embryos was not apparently impaired, as hatching occurred in 44% of the former and 20% of the latter, compared with 18.1% in non-manipulated controls. The biopsied cells (approximately 10-30) usually remained in a clump but 14% formed vesicles on the day after biopsy. There was, however, no evidence of adherence to the dish or formation of monolayers. These results demonstrate the feasibility of trophectoderm biopsy in human blastocysts and that sufficient extra-embryonic material can be obtained by this technique for preimplantation diagnosis of genetic disorders.read more
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Journal ArticleDOI
Preimplantation genetic diagnosis
TL;DR: An overview of indications for, and techniques used in, PGD is provided and results obtained with the technique and outcomes of pregnancies are discussed, and a brief review of new technologies is included.
Journal ArticleDOI
Cleavage-stage biopsy significantly impairs human embryonic implantation potential while blastocyst biopsy does not: a randomized and paired clinical trial.
TL;DR: Cleavage-stage biopsy markedly reduced embryonic reproductive potential and in contrast, trophectoderm biopsy had no measurable impact and may be used safely when embryo biopsy is indicated.
Journal ArticleDOI
ESHRE PGD Consortium ‘Best practice guidelines for clinical preimplantation genetic diagnosis (PGD) and preimplantation genetic screening (PGS)’
Alan R. Thornhill,C.E. deDie-Smulders,Joep P.M. Geraedts,Joyce C. Harper,Gary Harton,S.A. Lavery,Céline Moutou,M.D. Robinson,Andreas G. Schmutzler,Paul N. Scriven,Karen Sermon,Leeanda Wilton +11 more
TL;DR: The European Society of Human Reproduction and Embryology (ESHRE) as discussed by the authors developed guidelines for preimplantation genetic diagnosis (PGD), which are aimed at giving information, support and guidance to potential, fledgling and established PGD centres.
Journal ArticleDOI
Preimplantation genetic screening: a systematic review and meta-analysis of RCTs
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Current Practices and Controversies in Assisted Reproduction
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