Journal ArticleDOI
Urinary 3H-Tetracycline and Pyridinium Crosslinks Differ in Their Response to Calcium Restriction in Mature and Aged Rats
TLDR
Data indicate that although both markers are elevated by CR, marker response differs with age, and variability exists for acute and chronic responses.Abstract:
The aim of this study was to evaluate bone resorption (BR) in rats by two methods: chronic 3H-tetracycline labeling (3HTC) and pyridinium crosslink excretion (PYDX), and compare the sensitivity of these markers in two age groups. Female Sprague-Dawley rats at 12–29 weeks of age (``mature'', n = 12) and at 40–57 weeks of age (``aged'', n = 22) were examined. Skeletal incorporation of 3HTC in aged rats was 43 ± 8% of that in mature animals (P < 0.01), indicating an age-related decrease in bone turnover. BR was modulated over 9 weeks by calcium restriction (CR), measured by urinary excretion of both 3HTC and PYDX, and compared with age-matched, calcium-adequate controls. At baseline, urinary excretion of 3HTC was not significantly different between age groups, whereas urinary PYDX was 14–20% higher in mature compared with aged rats (P < 0.01). CR produced a 32–39% peak increase in BR (P < 0.01) compared with controls that did not differ significantly between marker or age group. Urinary 3HTC was elevated at weeks 1–3 (P < 0.01) and reached maximal values at week 2 (32 ± 17%). Urinary PYDX, however, was not elevated until week 2, reached maximal levels at week 3 (39 ± 15%), and remained elevated until week 6 (P < 0.01). These data indicate that although both markers are elevated by CR, marker response differs with age, and variability exists for acute and chronic responses.read more
Citations
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Journal ArticleDOI
The effect of calcium intake on bone composition and bone resorption in the young growing rat
Annette Creedon,Kevin D. Cashman +1 more
TL;DR: It is suggested that increasing dietary Ca intake, well above the recommended level, had no effect on bone mineral composition or bone resorption in young growing female rats.
Journal ArticleDOI
Peak bone strength is influenced by calcium intake in growing rats
S. Viguet-Carrin,M. Hoppler,F. Membrez Scalfo,J. Vuichoud,M. Vigo,Elizabeth A. Offord,Patrick Ammann +6 more
TL;DR: The results suggest that supplemental Ca may be beneficial for the attainment of peak bone strength and that multiple factors linked to bone mass and strength should be taken into account when setting dietary levels of adequate mineral intake to support optimal peak bone mass acquisition.
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Calcium and Magnesium Supplementation Improves Serum OPG/RANKL in Calcium-Deficient Ovariectomized Rats
Yun Jung Bae,Mi-Hyun Kim +1 more
TL;DR: It is still unclear whether Mg supplementation can improve bone mineral status, while M g supplementation with an adequate-Ca diet resulted in a change in cytokines that may promote bone formation.
Journal ArticleDOI
Bone Seeking Labels as Markers for Bone Turnover: Effect of Dosing Schedule on Labeling Various Bone Sites in Rats
Jennifer M. K. Cheong,Nilupa S. Gunaratna,George P. McCabe,George S. Jackson,Connie M. Weaver +4 more
TL;DR: This study affirms that a single administration of either 3H-TC or 45Ca is a useful approach to measuring bone turnover directly and the amount of label incorporation into bone was greater in bone sites that were more metabolically active and in all sites when closer vs farther from OVX.
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Effect of inulin-type fructans on body weight gain and selected biochemical parameters at calcium hypoalimentation in rats.
TL;DR: The results showed that the level of total and ionised calcium, magnesium and phosphorus in blood serum as well as Ca content in femur varied with the amount of Ca in rat diet.
References
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Journal ArticleDOI
Quantitative analysis of the pyridinium crosslinks of collagen in urine using ion-paired reversed-phase high-performance liquid chromatography
TL;DR: A method has been developed for the simultaneous and rapid analysis of the 3-hydroxypyridinium crosslinks of mature collagen, pyrid inoline and deoxypyrIDinoline, in samples of urine and tissue and its application in the study of degenerative disorders of bone and connective tissue is illustrated.
Journal ArticleDOI
Excretion of pyridinium cross-links of collagen in ovariectomized rats as urinary markers for increased bone resorption.
TL;DR: Measurements of free pyrid inoline and deoxypyridinoline in urine appear to provide a good index of the increased bone resorption induced by estrogen deficiency.
Journal ArticleDOI
Assay of bone resorption in vivo with3H-tetracycline
LeRoy Klein,Kenneth Van Jackman +1 more
TL;DR: 3H-Tetracycline was used to quantify resorption in whole bones of growing rats and dogs, suggesting thatabsolute amounts of45Ca resorbed from bone, as reflected by losses of 3H-TC, are five to ten times greater than thenet amounts of 45Ca lost from bone.
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