Using Lamm-Equation Modeling of Sedimentation Velocity Data to Determine the Kinetic and Thermodynamic Properties of Macromolecular Interactions
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TLDR
Herein, the SV analytical advances implemented in the software package SEDPHAT are summarized and detailed analyses of SV data using these strategies are presented.About:
This article is published in Methods.The article was published on 2011-05-01 and is currently open access. It has received 42 citations till now. The article focuses on the topics: Lamm equation.read more
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Book ChapterDOI
Calculations and Publication-Quality Illustrations for Analytical Ultracentrifugation Data.
TL;DR: The analysis of analytical ultracentrifugation data has been greatly facilitated by the advances accumulated in recent years, and the significant organizational and logistical hurdles presented by these approaches are streamlined by the software described herein, called GUSSI.
Journal ArticleDOI
Overview of current methods in sedimentation velocity and sedimentation equilibrium analytical ultracentrifugation.
TL;DR: In this paper, the authors describe the current tools for the study of soluble proteins, detergent-solubilized membrane proteins and their interactions by sedimentation velocity and sedimentation equilibrium.
Journal ArticleDOI
Protein–protein interactions: switch from classical methods to proteomics and bioinformatics-based approaches
Armand G. Ngounou Wetie,Izabela Sokolowska,Alisa G. Woods,Urmi Roy,Katrin Deinhardt,Costel C. Darie +5 more
TL;DR: A strong case is made as to why unraveling the interactome is the next challenge in the field of proteomics and classical methods of investigation of PPIs and structure-based bioinformatics approaches are presented.
Journal ArticleDOI
Structure and Dynamics of Full-Length HIV-1 Capsid Protein in Solution.
Lalit Deshmukh,Charles D. Schwieters,Alexander Grishaev,Rodolfo Ghirlando,James L. Baber,G. Clore +5 more
TL;DR: The structure and dynamics of full-length capsid protein in solution, comprising a mixture of monomeric and dimeric forms in dynamic equilibrium, are studied using ensemble simulated annealing driven by experimental NMR residual dipolar couplings and X-ray scattering data.
Journal ArticleDOI
Combining biophysical methods for the analysis of protein complex stoichiometry and affinity in SEDPHAT
Huaying Zhao,Peter Schuck +1 more
TL;DR: The principles and recent developments of biophysical solution methods implemented for GMMA in the software SED PHAT are reviewed, their complementarity in GMMA is described and a new GMMA simulation tool set in SEDPHAT is presented.
References
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Book
Data Reduction and Error Analysis for the Physical Sciences
TL;DR: In this paper, Monte Carlo techniques are used to fit dependent and independent variables least squares fit to a polynomial least-squares fit to an arbitrary function fitting composite peaks direct application of the maximum likelihood.
Journal ArticleDOI
Data Reduction and Error Analysis for the Physical Sciences.
TL;DR: Numerical methods matrices graphs and tables histograms and graphs computer routines in Pascal and Monte Carlo techniques dependent and independent variables least-squares fit to a polynomial least-square fit to an arbitrary function fitting composite peaks direct application of the maximum likelihood.
Book
Current protocols in immunology
TL;DR: Current Protocols in Immunology is a three-volume looseleaf manual that provides comprehensive coverage of immunological methods from classic to the most cutting edge, including antibody detection and preparation, assays for functional activities of mouse and human cells involved in immune responses, and animal models of autoimmune and inflammatory diseases.
Journal ArticleDOI
How to measure and predict the molar absorption coefficient of a protein.
TL;DR: The studies reported here suggest that the Edelhoch method is the best method for measuring ε for a protein, which can best be predicted with this equation.
Journal ArticleDOI
Size-Distribution Analysis of Macromolecules by Sedimentation Velocity Ultracentrifugation and Lamm Equation Modeling
TL;DR: A new method for the size-distribution analysis of polymers by sedimentation velocity analytical ultracentrifugation that exploits the ability of Lamm equation modeling to discriminate between the spreading of the sedimentation boundary arising from sample heterogeneity and from diffusion is described.