Vir typing: a long-PCR typing method for group A streptococci.
Donald L. Gardiner,J Hartas,Bart J. Currie,John D. Mathews,David J. Kemp,Kadaba S. Sriprakash +5 more
TLDR
A new procedure for typing Streptococcus pyogenes, by amplifying the entire 5- to 7-kb variable vir regulon by long PCR, and the stoichiometric yield of restriction fragments in Vir typing allows unambiguous interpretation of results.Abstract:Ā
We have developed a new procedure (Vir typing) for typing Streptococcus pyogenes, by amplifying the entire 5- to 7-kb variable vir regulon by long PCR. The amplified DNA is then cleaved with HaeIII and visualized by ethidium bromide fluorescence after agarose gel electrophoresis. A simple procedure for preparing DNA of sufficiently high quality from 96 samples was employed simultaneously. This DNA was also used to develop a random amplified polymorphic DNA (RAPD) procedure. The discriminatory power of the two DNA-based procedures was compared with previous methods, M typing, and multilocus enzyme electrophoresis. Both procedures were highly discriminatory, but the stoichiometric yield of restriction fragments in Vir typing allows unambiguous interpretation of results.read more
Citations
More filters
Journal ArticleDOI
Multilocus Sequence Typing of Streptococcus pyogenes and the Relationships between emm Type and Clone
TL;DR: The findings indicate that the majority of emm types examined define clones or clonal complexes, similar to other multi-drug-resistant microorganisms.
Journal ArticleDOI
Nontypeable Haemophilus influenzae in the Lower Respiratory Tract of Patients with Chronic Bronchitis
Venkata Bandi,Michael A. Apicella,Edward O. Mason,Timothy F. Murphy,Ather Siddiqi,Robert L. Atmar,Stephen B. Greenberg +6 more
TL;DR: The frequency of colonization and intracellular localization of nontypeable Haemophilus influenzae (NTHi) in the lower respiratory tract was determined in healthy adults and in clinically stable and acutely ill chronic bronchitis patients, suggesting a role for intrACEllular infection by NTHi in the pathogenesis of exacerbations of CB.
Journal ArticleDOI
New multi-determinant strategy for a group A streptococcal vaccine designed for the Australian Aboriginal population.
Evelyn R. Brandt,Kadaba S. Sriprakash,Rhonda I. Hobb,Wendy A. Hayman,Weiguang Zeng,Michael R. Batzloff,David C. Jackson,Michael F. Good +7 more
TL;DR: This work linked seven serotypic peptides with J14 using a new chemistry technique that enables the immunogen to display all the individual peptides pendant from an alkane backbone, and demonstrated excellent immunogenicity and protection in mice.
Book
Laboratory diagnosis of group A streptococcal infections
TL;DR: By helping to promote and protect health and prevent and control disease throughout the world, WHO's books contribute to achieving the Organiza-tion's principal objective-the attainment by all people of the highest possible level of health.
Journal ArticleDOI
The possibilities and limitations of nucleic acid amplification technology in diagnostic microbiology.
Mario Vaneechoutte,J. Van Eldere +1 more
TL;DR: It is predicted that-in contrast to research and reference facilities-routine bacteriology laboratories will continue to rely on culture as the preferred 'amplification method' for most diagnostic applications.
References
More filters
Journal ArticleDOI
A mathematical theory of communication
TL;DR: This final installment of the paper considers the case where the signals or the messages or both are continuously variable, in contrast with the discrete nature assumed until now.
Journal ArticleDOI
Separation of yeast chromosome-sized DNAs by pulsed field gradient gel electrophoresis
TL;DR: This pulsed field gradient gel electrophoresis fractionates intact S. cerevisiae chromosomal DNA, producing a molecular karyotype that greatly facilitates the assignment of genes to yeast chromosomes.
Journal ArticleDOI
PCR amplification of up to 35-kb DNA with high fidelity and high yield from lambda bacteriophage templates
TL;DR: The base-pair fidelity, the ability to use PCR products as primers, and the maximum yield of target fragment were increased and improvements were achieved by the combination of an exonuclease-free, N-terminal deletion mutant of Taq DNA polymerase, Klentaq1.
Journal ArticleDOI
Streptococcal M protein: molecular design and biological behavior.
TL;DR: Many of the approaches described in the elucidation of the M-protein structure may be applied for characterizing similar molecules in other microbial systems.
Journal ArticleDOI
Streptococcus pyogenes causing toxic-shock-like syndrome and other invasive diseases: clonal diversity and pyrogenic exotoxin expression.
James M. Musser,Alan R. Hauser,Michael H Kim,Patrick M. Schlievert,Kimberlyn Nelson,R. K. Selander +5 more
TL;DR: The demonstration of a relatively high frequency of expression of exotoxin A among isolates recovered from toxic-shock-like syndrome patients in the United States was confirmed, and this association both within clones and among distantly related clones supports the hypothesis that exot toxin A is a causal factor in pathogenesis of this disease.