Journal ArticleDOI
Water distribution in incubated slices of brain and other tissues.
TLDR
With glutamate or high potassium in the medium, swelling is increased and the non-thiocyanate, non-sucrose, and non-inulin spaces increase correspondingly, and this increase is apparently intracellular.Abstract:
Slices of rat cerebral cortex swell about 40% during one hour's aerobic incubation in bicarbonate-buffered medium. Thiocyanate or sucrose added to the medium equilibrate with part of the tissue flu...read more
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Journal ArticleDOI
The influence of drugs and potassium on respiration and potassium accumulation by brain tissue
TL;DR: When brain cortex slices in which the potassium content has been reduced by cold-pretreatment are incubated aerobically at 38° in a potassium-free glucose-containing medium, addition to the medium of 3.6 mM potassium causes an increase of the oxygen uptake rate accompanied by a reaccumulation of potassium in the tissue and an approximately equal extrusion of sodium.
Journal ArticleDOI
Changes in protein, electrolyte, and water metabolism caused by prolonged spreading cortical depression in rats
TL;DR: Repeated application of 25% KCl on the cerebral cortex for 5 hours led to a decrease of protein content (referred to both dry weight and DNA content) and the potassium and water content of the KCl treated cortex increased.
Journal ArticleDOI
Measurement of extracellular fluid volume using 131I labelled inulin
C.T.G. Flear,I.G. Graber +1 more
TL;DR: It is concluded that allyl inulin labelled with 131 I can be used to measure, in vitro, the volume of extracellular fluid in pieces of tissue, but the desirability is emphasised of standardised preparations of inulin with known molecular dispersion.
Journal ArticleDOI
In vitro incubation of brain hemispheres Fluid spaces and amino acid uptake
TL;DR: In the present paper, an in vitro incubation study was undertaken with cat brain hemispheres in toto, one covered by the meninx and the other devoid of this enveloping membrane, and it will be shown that the incorporation of amino acids in the naked hemisphere is higher than in the other.
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Fluid compartmentation and electrolytes of cat cerebral cortex in vitro–i swelling and solute distribution in mature cerebral cortex*
Robert S. Bourke,Donald B. Tower +1 more