Showing papers on "Melissa officinalis published in 2022"
TL;DR: The essential oil obtained from basil leaves grown under shading has shown the highest antioxidant activity (EC50 value after 20 min of incubation = 0.68 mg/mL).
Abstract: Lemon balm, mint, and basil were used to determine whether light modification (plants grown under pearl shade nets with 40 % shaded index or in un-shaded open field) could improve the quantity and quality of essential oils (EOs) and antioxidant activity. The yield of EOs of lemon balm, mint and sweet basil obtained after 120 min of hydrodistillation was 0.18, 2.00, and 1.12 mL/100 g, respectively. At the same time under shading conditions plants obtained more levels of EOs (0.22, 2.23, and 1.32 mL/100 g). The main components of the lemon balm essential oil are geranial (6.84–7.78 %); neral (3.02–3.52 %) and piperitenone oxide (1.67–5.36 %). The main components of mint essential oil are piperitenone oxide (12–14 %) and 1,8 cineole (1.32–2.27 %), while basil essential oil consists of the following components: linalool (9.06–10.2 %); 1,8 cineole (1.26–8.06 %) and α-trans bergamotene (1.21–1.47 %). The essential oil obtained from basil leaves grown under shading has shown the highest antioxidant activity (EC50 value after 20 min of incubation = 0.68 mg/mL). Medicinal plants are a good source of natural antioxidants with potential application in the food and pharmaceutical industries. Medicinal plants can be recommended to grow in shading conditions for achieving optimal quality parameters.
34 citations
TL;DR: In this paper , a study of lemon balm, mint, and basil was conducted to determine whether light modification (plants grown under pearl shade nets with 40 % shaded index or in un-shaded open field) could improve the quantity and quality of essential oils (EOs) and antioxidant activity.
Abstract: Lemon balm, mint, and basil were used to determine whether light modification (plants grown under pearl shade nets with 40 % shaded index or in un-shaded open field) could improve the quantity and quality of essential oils (EOs) and antioxidant activity. The yield of EOs of lemon balm, mint and sweet basil obtained after 120 min of hydrodistillation was 0.18, 2.00, and 1.12 mL/100 g, respectively. At the same time under shading conditions plants obtained more levels of EOs (0.22, 2.23, and 1.32 mL/100 g). The main components of the lemon balm essential oil are geranial (6.84–7.78 %); neral (3.02–3.52 %) and piperitenone oxide (1.67–5.36 %). The main components of mint essential oil are piperitenone oxide (12–14 %) and 1,8 cineole (1.32–2.27 %), while basil essential oil consists of the following components: linalool (9.06–10.2 %); 1,8 cineole (1.26–8.06 %) and α-trans bergamotene (1.21–1.47 %). The essential oil obtained from basil leaves grown under shading has shown the highest antioxidant activity (EC 50 value after 20 min of incubation = 0.68 mg/mL). Medicinal plants are a good source of natural antioxidants with potential application in the food and pharmaceutical industries. Medicinal plants can be recommended to grow in shading conditions for achieving optimal quality parameters. • Light intensity could improve the quantity and quality of essential oils. • Shading plants synthesized more EOs than un-shading plants. • The essential oil from shaded basil have shown the highest antioxidant activity.
31 citations
TL;DR: Melissa officinalis is a medicinal plant rich in biologically active compounds which is used worldwide for its therapeutic effects as discussed by the authors , it contains mainly flavonoids, terpenoids, phenolic acids, tannins, and essential oil.
Abstract: Melissa officinalis is a medicinal plant rich in biologically active compounds which is used worldwide for its therapeutic effects. Chemical studies on its composition have shown that it contains mainly flavonoids, terpenoids, phenolic acids, tannins, and essential oil. The main active constituents of Melissa officinalis are volatile compounds (geranial, neral, citronellal and geraniol), triterpenes (ursolic acid and oleanolic acid), phenolic acids (rosmarinic acid, caffeic acid and chlorogenic acid), and flavonoids (quercetin, rhamnocitrin, and luteolin). According to the biological studies, the essential oil and extracts of Melissa officinalis have active compounds that determine many pharmacological effects with potential medical uses. A new field of research has led to the development of controlled release systems with active substances from plants. Therefore, the essential oil or extract of Melissa officinalis has become a major target to be incorporated into various controlled release systems which allow a sustained delivery.
20 citations
TL;DR: In this article , the influence of various soil pH on the biological parameters and uptake of manganese, copper and zinc by basil, dandelion and lemon balm was assessed.
Abstract: Herbs used in medicine should be grown under controlled and standardized conditions. Agricultural practices often induce changes to soil pH, which may affect migration of heavy metals in the environment, their accumulation in plant tissues and the concentration of medicinal ingredients. The aim of this work was to assess the influence of various soil pH on the biological parameters and uptake of manganese, copper and zinc by basil, dandelion and lemon balm. The soil analysis covered pH, organic matter content, bioavailable and total forms of investigated metals in soil. In plants cultivated in soil at pH covering the range 4.7–8.5 the concentrations of Mn, Cu and Zn were analyzed. Their mobility and availability were assessed by bioaccumulation factors, translocation factors and transfer coefficients. The seed germination and subsequent herbs growth were strongly dependent on soil pH for all investigated plant species. Photosynthetic efficiency at different pHs was positively correlated with uptake of Cu and Mn while Zn behaved in a more random way.
12 citations
TL;DR: In this paper , the authors investigated the impact of a number of LED light spectra (red, blue, green and white) on the growth, development and essential oil content of lemon balm (Melissa officinalis), a herb and pharmaceutical plant species used across the world.
Abstract: With the recent development of LED lighting systems for plant cultivation, the use of vertical farming under controlled conditions is attracting increased attention. This study investigated the impact of a number of LED light spectra (red, blue, green and white) on the growth, development and essential oil content of lemon balm (Melissa officinalis), a herb and pharmaceutical plant species used across the world. White light and red-rich light spectra gave the best outputs in terms of impact on the growth and yield. For blue-rich spectra, the development and yield was lower despite having a significant impact on the photosynthesis activity, including Fv/Fm and NDVI values. For the blue-rich spectra, a peak wavelength of 450 mn was better than that of 435 nm. The results have practical value in terms of increased yield and the reduction of electricity consumption under controlled environmental conditions for the commercial production of lemon balm.
10 citations
TL;DR: In this paper , the authors evaluated the antimicrobial impacts on Melissa officinalis L. essential oil (MOEO) against Vibrio parahaemolyticus, and found that MOEO exerted its antimicrobial effect mainly by destroying the membrane.
Abstract: The study was to evaluate the antimicrobial impacts on Melissa officinalis L. essential oil (MOEO) against Vibrio parahaemolyticus. The minimum inhibitory concentration (MIC) of MOEO on Vibrio parahaemolyticus was 1 μL⋅mL–1. The kill-time curve exhibited that MOEO had good antimicrobial activity. The analysis of cellular ingredients leakage and cell viability illustrated that MOEO has destruction to the morphology of the cell membrane. The damage to the membrane integrity by MOEO has been confirmed by transmission and scanning electron microscopy, obvious morphological and ultrastructural changes were observed in the treated bacterial cells. The MOEO at 0.5 μL⋅mL–1 can inhibit the biofilm formation, biofilm motility, and extracellular polysaccharide production. Meanwhile, the qPCR results exhibited MOEO inhibited the expression of virulence genes. The findings showed that MOEO exerted its antimicrobial effect mainly by destroying the membrane, which indicated its potential as a natural food preservative.
8 citations
TL;DR: In this article, the authors evaluated the effect of MO essential oil on the gastrointestinal and heart-related symptoms accompanying anxiety disorders, in vitro models were utilized that follow the function of the isolated mouse ileum and atria tissues, respectively.
7 citations
TL;DR: In this paper , the authors evaluated the effect of MO essential oil on the gastrointestinal and heart-related symptoms accompanying anxiety disorders, in vitro models were utilized that follow the function of the isolated mouse ileum and atria tissues, respectively, after exposure to Mo essential oil.
7 citations
TL;DR: The results suggest that plants from the unexplored flora of Cyprus could serve as a source for identifying novel anti-QS inhibitors to treat infectious diseases caused by pathogens that are resistant to antibiotics.
Abstract: Quorum sensing (QS) is a form of intra- and inter-species communication system employed by bacteria to regulate their collective behavior in a cell population-dependent manner. QS has been implicated in the virulence of several pathogenic bacteria. This work aimed to investigate the anti-QS potential of ethanolic extracts of eight aromatic plants of Cyprus, namely, Origanum vulgare subsp. hirtum, Rosmarinus officinalis, Salvia officinalis, Lavendula spp., Calendula officinalis, Melissa officinalis, Sideritis cypria, and Aloysia citriodora. We initially assessed the effects of the extracts on autoinducer 2 (AI-2) signaling activity, using Vibrio harveyi BB170 as a reported strain. We subsequently assessed the effect of the ethanolic extracts on QS-related processes, including biofilm formation and the swarming and swimming motilities of Escherichia coli MG1655. Of the tested ethanolic extracts, those of Origanum vulgare subsp. hirtum, Rosmarinus officinalis, and Salvia officinalis were the most potent AI-2 signaling inhibitors, while the extracts from the other plants exhibited low to moderate inhibitory activity. These three ethanolic extracts also inhibited the biofilm formation (>60%) of E. coli MG1655, as well as its swimming and swarming motilities, in a concentration-dependent manner. These extracts may be considered true anti-QS inhibitors because they disrupt QS-related activities of E. coli MG1655 without affecting bacterial growth. The results suggest that plants from the unexplored flora of Cyprus could serve as a source for identifying novel anti-QS inhibitors to treat infectious diseases caused by pathogens that are resistant to antibiotics.
7 citations
TL;DR: This review has been prepared with a specific research strategy, interrogating different databases with the keyword M. officinalis and analyzes the properties of this plant updating currently available literature, with a special emphasis on human studies.
Abstract: Melissa officinalis L. is a plant of the Lamiaceae family known in numerous countries for its medicinal activities. This plant has been used since ancient times to treat different disorders, including gastrointestinal, cardiovascular, neurological, psychological conditions. M. officinalis contains several phytochemicals such as phenolic acids, flavonoids, terpenoids, and many others at the basis of its pharmacological activities. Indeed, the plant can have antioxidant, anti-inflammatory, antispasmodic, antimicrobial, neuroprotective, nephroprotective, antinociceptive effects. Given its consolidated use, M. officinalis has also been experimented with clinical settings, demonstrating interesting properties against different human diseases, such as anxiety, sleeping difficulties, palpitation, hypertension, depression, dementia, infantile colic, bruxism, metabolic problems, Alzheimer's disease, and sexual disorders. As for any natural compound, drug, or plant extract, also M. officinalis can have adverse effects, even though the reported events are very rare and the plant can be considered substantially safe. This review has been prepared with a specific research strategy, interrogating different databases with the keyword M. officinalis. Moreover, this work analyzes the properties of this plant updating currently available literature, with a special emphasis on human studies.
6 citations
TL;DR: In this article , the authors highlighted M. officinalis essential oil's antioxidant potential and antimicrobial activity against L. monocytogenes, presenting a bactericidal action and being able to inhibit some virulence attributes.
TL;DR: In this article , the effects of methyl jasmonate (MeJA) on RA content, total phenolic content, and total flavonoid content (TFC), as well as changes in expression of their biosynthesis-related key genes (MoPAL, Mo4CL, and MoRAS) were investigated.
Abstract: The medicinal herb, lemon balm (Melissa officinalis L.), which is high in rosmarinic acid (RA), has well-known therapeutic value. The goals of this study were to investigate the effects of methyl jasmonate (MeJA) on RA content, total phenolic content (TPC), and total flavonoid content (TFC), as well as changes in expression of their biosynthesis-related key genes (MoPAL, Mo4CL, and MoRAS) in Iranian lemon balm ecotypes, as first reported. Our results revealed that MeJA doses significantly increase the RA content, TPC, and TFC in both ecotypes compared with the control samples. Additionally, the higher expression levels of MoPAL, Mo4CL, and MoRAS following treatment were linked to RA accumulation in all treatments for both Iranian lemon balm ecotypes. After 24 h of exposure to 150 µM MeJA concentration, HPLC analysis showed that MeJA significantly increased RA content in Esfahan and Ilam ecotypes, which was about 4.18- and 7.43-fold higher than untreated plants. Our findings suggested that MeJA has a considerable influence on RA, TPC, and TFC accumulation in MeJA-treated Iranian M. officinalis, which might be the result of gene activation from the phenylpropanoid pathway. As a result of our findings, we now have a better understanding of the molecular processes behind RA production in lemon balm plants.
TL;DR: In this article , the authors developed active films based on carboxymethyl chitosan (CMCS)/locust bean gum (LBG) films containing Melissa officinalis L. essential oil (MOEO) nanoemulsions, which resulted in an increase in the elongation of break, water resistance and improved the film hydrophilicity.
Abstract: This study aimed to develop active films based on carboxymethyl chitosan (CMCS)/locust bean gum (LBG) films containing Melissa officinalis L. essential oil (MOEO) nanoemulsions. The results showed that the active films incorporated with MOEO nanoemulsion resulted in an increase in the elongation of break, water resistance and improved the film hydrophilicity. Elongation of break increased from 18.49% to 27.97% with the addition of 4% MOEO nanoemulsion. Water resistance was decreased from 56.32% to 25.43%, and water contact angle was increased from 75.13 to 83.86 with the addition of 4% MOEO nanoemulsion. However, the water vapor barrier properties and tensile strength decreased with the addition of MOEO nanoemulsions. The scanning electron microscopic images and Fourier transform infrared spectroscopy results showed that the MOEO was very compatible with the film materials and dispersed evenly in the films. At the same time, the addition of MOEO nanoemulsion significantly enhanced antioxidant and antibacterial activities of C/L-MOEO films. The antioxidant and antimicrobial activities of C/L-MOEO films were increased from 7.16% to 33.81% and 3.52% to 54.50%, respectively. In general, C/L-MOEO film has great application prospects.
TL;DR: The usefulness of 3 herbicides and flame weeding in lemon balm sown directly into the field was assessed in a two-year experiment as discussed by the authors , and the most effective method was spraying with glufosinate-ammonium, which controlled 69-76% of weeds.
Abstract: The usefulness of 3 herbicides and flame weeding in lemon balm sown directly into the field was assessed in a two-year experiment. Glufosinate-ammonium (600 g∙ha–1) and flame weeding (90 kg propane∙ha–1) were applied 12–13 days after lemon balm sowing, i.e. shortly after weed emergence and 4 days before crop emergence. Bentazon (960 g∙ha–1) and fluazifop-P-butyl (150 g∙ha–1) were sprayed approximately 3 weeks after sowing, during the emergence of lemon balm, and when the weeds were in the cotyledon – 2–4 true leaves stage. All of the studied weed control methods significantly reduced the number and fresh weight of weeds growing 4 weeks after lemon balm sowing. The most effective method was spraying with glufosinate-ammonium, which controlled 69–76% of weeds. The efficiency of flame weeding was slightly lower. Bentazon caused slight, temporary chlorosis of some lemon balm cotyledons. Content of essential oil (1.9–2.1%), its composition and content of rosmarinic acid (2.08–2.44%) in lemon balm leaves, as well as content of total nitrogen (2.18–2.55%), phosphorus (0.30–0.32%), potassium (2.94–3.22%), calcium (1.02–1.60%), and magnesium (0.30–0.32%) in lemon balm raw material were independent of the weeding method. Content of essential oil, phosphorus, potassium and calcium were significantly higher in the dryer year. Studied weed control methods proved useful in the cultivation of lemon balm from direct sowing into the field. Methods with total action (flaming and glufosinate-ammonium) were more effective than those with selective herbicides (bentazon and fluazifop-P-butyl).
TL;DR: In this article, a randomized placebo-controlled double-blind trial aimed to assess the effects of rosmarinic acid (RA) extract on cognition in older adults without dementia.
Abstract: BACKGROUND
Previous in vitro and in vivo studies on Alzheimer's disease (AD) models have reported that rosmarinic acid (RA) can inhibit the formation of amyloid-β fibrils as well as the oligomerization and deposition of amyloid-β protein. Melissa officinalis (M. officinalis) extract containing 500 mg of RA is tolerable and safe in healthy individuals and patients with mild AD dementia.
OBJECTIVE
This randomized placebo-controlled double-blind trial aimed to assess the effects of M. officinalis extract on cognition in older adults without dementia.
METHODS
This study included individuals who were diagnosed with subjective or mild cognitive impairment (n = 323). The trial involved M. officinalis extract supplementation (500 mg of RA per day) period of 96 weeks followed by a washout period of 24 weeks. The primary endpoint was the Alzheimer's Disease Assessment Scale-cognitive subscale score, and the secondary endpoints were other cognitive measure results as well as safety and tolerability.
RESULTS
There were no significant differences in cognitive measures between the placebo and M. officinalis groups from baseline to 96 weeks. However, based on the analysis of Clinical Dementia Rating Sum of Boxes scores in participants without hypertension, the score was found to be increased by 0.006 and decreased by 0.085 in the M. officinalis and placebo groups, respectively; this difference was statistically significant (p = 0.036). Furthermore, there were no differences in vital signs, physical and neurological measures, or hippocampal volume between the two groups.
CONCLUSION
These results indicate that M. officinalis extract may help prevent cognitive decline in older adults without hypertension.
TL;DR: In this article , a comprehensive and systematic review of evidence-based use of herbal medicine for the treatment of Attention Deficit Hyperactivity Disorder (ADHD) in children and adolescents is presented.
Abstract: Attention deficit hyperactivity disorder (ADHD) is commonly a neurodevelopmental behavioural disorder in children and adolescents. Mainly characterized by symptoms like lack of attention, hyperactivity, and impulsiveness, it can impact the overall mental development of the one affected. Several factors, both genetic and non-genetic, can be responsible for this disorder. Although several traditional treatment methods involve medication and other counselling techniques, they also come with different side effects. Hence, the choice is now shifting to alternative treatment techniques. Herbal treatments are considered one of the most popular complementary and alternative medicine (CAM) administered. However, issues related to the safety and efficacy of herbal remedies for the treatment of ADHD need to be investigated further. This study aims to find out the recent advancement in evidence-based use of herbal remedies for ADHD by a comprehensive and systematic review that depicts the results of the published works on herbal therapy for the disorder. The electronic databases and the references retrieved from the included studies present related randomized controlled trials (RCTs) and open-label studies. Seven RCTs involving children and adolescents diagnosed with ADHD met the inclusion criteria. There is a fair indication of the efficacy and safety of Melissa officinalis L., Bacopa monnieri (L.) Wettst., Matricaria chamomilla L., and Valeriana officinalis L. from the studies evaluated in this systematic review for the treatment of various symptoms of ADHD. Limited evidence was found for Ginkgo biloba L. and pine bark extract. However, various other preparations from other plants did not show significant efficacy. There is inadequate proof to strongly support and recommend the administration of herbal medicines for ADHD, but more research is needed in the relevant field to popularize the alternative treatment approach.
TL;DR: In this paper , plant essential oils (EOs) were used as sprout inhibitors or suppressors in potato (Solanum tuberosum L. officinalis) using the wicked method on potato cv. Gala in 19 L plastic containers.
Abstract: The goal of this research was to screen plant essential oils (EOs) as sprout inhibitors or suppressors in potato (Solanum tuberosum L.). Three controlled environment experiments were conducted to screen 18 EOs and several pure compounds as sprout inhibitors. The EOs were applied using the wicked method on potato cv. Gala in 19 L plastic containers. The results indicated that Melissa officinalis L. EO inhibited sprouting, while Coriandrum sativum L. seed oil and the EO blend of Lavandula angustifolia Mill. and Salvia sclarea L. suppressed sprouting. The EOs of interest were analyzed using gas chromatography coupled to mass spectrometry (GC-MS) and/or a flame ionization detector (GC-FID); the detailed chemical profiles are provided. The M. officinalis EO was fractionated into seven fractions, and these were tested on minitubers. We identified two fractions (F and A) that suppressed potato sprouting better than the whole oil. The GC-MS-FID analyses of M. officinalis EO fraction A identified myrcene, Z-ocimene, E-ocimene, trans-caryophyllene, and α-humulene as the main constituents, while the main constituents of fraction F were α-terpineol, β-citronellol, and geraniol. The pure isolated compounds, together with the major compound in M. officinalis EO (citral), were tested for sprout suppression on three potato cultivars (Ranger Russet, Terra Rosa, and Dakota TrailBlazer), which revealed that β-citronellol reduced the sprout length and the number of sprouts in all three cultivars, while citral and (+)-α-terpineol reduced the sprout length and the number of sprouts in Ranger Russet relative to the two controls in all three cultivars. Myrcene had a stimulating effect on the number of sprouts in Cv. Terra Rosa. However, none of the pure compounds suppressed sprouting completely or were comparable to the EO of M. officinalis.
TL;DR: In this paper , the anti-inflammatory and neuroprotective activity of a standardized Melissa officinalis L. phytocomplex extract (MD) obtained with an in vitro plant cell culture was evaluated.
TL;DR: In this paper , the authors have shown that macroporous resins such as Amberlite XAD 1180N, XAD 7HP, and FPX66 are effective in extracting the anthocyanins, polyphenols, and flavonoids from aronia mitschurinii.
Abstract: Antioxidants play a vital role in the human body by defending cells from damage caused by free radicals, highly reactive products of oxidation reactions. A major source of antioxidants is fruits and vegetables. Aronia mitschurinii, a breed created at the end of the 19th century by crossbreeding wild Aronia melanocarpa and Russian Mountain Ash, produces fruits with one of the highest known content of hydrophilic antioxidants. Aronia fruit contains a potent blend of anthocyanins, polyphenols, and flavonoids. The most popular way of consuming the fruit is through juicing. Yet, due to very high concentrations of tannins in the juice, very few food-related applications have been developed. Resin extraction of antioxidants provides an alternative for utilizing valuable phytochemicals from crops for applications in the food industry as nutraceutical supplements and more. To increase the market value of the plant, it is important to determine what resins can extract the optimum concentration of antioxidants from aronia juice, pulp, and whole berries. We have shown that macroporous resins such as Amberlite XAD 1180N, Amberlite XAD 7HP, Amberlite XAD 761, and Amberlite FPX66, which have been reported to be effective in extracting the anthocyanins and polyphenols from other fruit juices, skins of red grapes, and the wild breed, are also effective for use in juice, pulp, and whole fruits of Aronia mitchurinii. However, the extremely high content of antioxidants presents a challenge to obtaining high recovery; a notable change in the juice/resin ratio is required to obtain a higher recovery value. Our results showed that Amberlite FPX66 was the best at extracting anthocyanins, polyphenols, and flavonoids from aronia juice. A separate experiment conducted to determine how to optimize the efficiency of FPX66 extraction revealed that increasing the resin/juice ratio increased the percent recovery of anthocyanins from aronia juice. Moreover, we have compared recovery between juice, pulp, and whole aronia berries and batch versus column extraction.
TL;DR: In this paper , two years experiment were conducted in 2016-2017 as split-plot based on randomized completely block design with three replications to morpho-physiological responses of Lemon Balm (Melissa officinalis L.) plant to mycorrhizal fungi species treatments (control, Rhizophagus intraradices, Funneliformis mosseae, Glomus hoi and combined application of all three species) under different irrigation regimes (irrigation after 70, 100, 130 and 160 mm evaporation).
Abstract: Two years experiment were conducted in 2016-2017 as split-plot based on randomized completely block design with three replications to morpho-physiological responses of Lemon Balm (Melissa officinalis L.) plant to mycorrhizal fungi species treatments (control, Rhizophagus intraradices, Funneliformis mosseae, Glomus hoi and combined application of all three species) under different irrigation regimes (irrigation after 70, 100, 130 and 160 mm evaporation). In results, the highest oil yield was achieved in irrigation at 100mm+application of all three species of mycorrhizal fungi. Irrigation after 100 mm evaporation increased this trait by 33% compared to irrigation after 70 mm evaporation due to increase in essential oil percentage under dehydration conditions, however, increasing drought stress led to a significant decrease in essential oil yield. In terms of physiological parameters, dehydration led to an increase in proline content and antioxidant activates. In general and according to the results, modifying the destructive effects of water deficit stress and the use of mycorrhiza can increase the essential oil of lemon Balm. But aggravating water deficiency conditions can drastically reduce the essential oil yield. The founds could be helpful for herbal medicine researchers to achieve high-quality drugs.
TL;DR: In this article , the content of active components, antioxidant, and anti-inflammatory potential of three different lemon balm extracts (MOEs), E1, E2, and E3), obtained under reflux and their effects on systemic redox status after acute per os administration in vivo post-carrageenan application.
Abstract: Melissa officinalis L. (MO), traditionally referred to as lemon balm, is one of the lemon-scent aromatic herbs widely used in traditional medicine due to its calming, sedative, and anti-arrhythmic effects. Furthermore, several studies have linked its therapeutic potential with its antioxidant properties. Here, we aimed to evaluate and compare the content of active components, antioxidant, and anti-inflammatory potential of three different MO extracts (MOEs), ethanolic macerate (E1), aqueous (E2), and ethanolic (E3), obtained under reflux and their effects on systemic redox status after acute per os administration in vivo post-carrageenan application. The HPLC analysis revealed that the most abundant constituent in all the three extracts was rosmarinic acid (RA), with higher content in E1 and E3 than in E2 (P < 0.05). The highest flavonoid content was found in the aqueous extract, especially quercetin (P < 0.05). For the carrageenan-induced paw edema model, dark agouti rats were used and divided into the groups: Control, indomethacin, E1, E2, and E3 subgrouped according to applied doses: 50, 100, and 200 mg/kg. Ethanolic macerate (E1200) and aqueous (E2100) MOE were shown to be anti-inflammatory agents in the carrageenan paw edema model, with the most prominent edema inhibition in the sixth hour post-carrageenan (63.89% and 69.44%, respectively, vs. 76.67% in the indomethacin group). All the three extracts reduced the production of pro-oxidants H2O2 and TBARS post-carrageenan and increased GSH levels compared to control (P < 0.05). These data imply the possible future usage of MOEs to prevent inflammatory and oxidative stress-related diseases.
TL;DR: Application of the NHE1 activator-containing cream significantly improved skin barrier functions by reducing skin surface pH and transepidermal water loss and increasing skin hydration, compared to patients who applied vehicle cream and those receiving no treatment.
Abstract: Stratum corneum (SC) pH regulates skin barrier functions and elevated SC pH is an important factor in various inflammatory skin diseases. Acidic topical formulas have emerged as treatments for impaired skin barriers. Sodium proton exchanger 1 (NHE1) is an important factor in SC acidification. We investigated whether topical applications containing an NHE1 activator could improve skin barrier functions. We screened plant extracts to identify NHE1 activators in vitro and found Melissa officinalis leaf extract. Rosmarinic acid, a component of Melissa officinalis leaf extract, significantly increased NHE1 mRNA expression levels and NHE1 production. Immunofluorescence staining of NHE1 in 3D-cultured skin revealed greater upregulation of NHE1 expression by NHE1 activator cream, compared to vehicle cream. Epidermal lipid analysis revealed that the ceramide level was significantly higher upon application of the NHE1 activator cream on 3D-cultured skin, compared to application of a vehicle cream. In a clinical study of 50–60-year-old adult females (n = 21), application of the NHE1 activator-containing cream significantly improved skin barrier functions by reducing skin surface pH and transepidermal water loss and increasing skin hydration, compared to patients who applied vehicle cream and those receiving no treatment. Thus, creams containing NHE1 activators, such as rosmarinic acid, could help maintain or recover skin barrier functions.
TL;DR: Herb remedies alone or in combination with curcumin, low-fat diet, NuAD-Trail, and soy lecithin showed significant positive effects on AD, and combination of natural and pharmaceuticals has far better effects than only allopathic treatment.
TL;DR: In this paper , the antimicrobial effect of plant to evaluate the in vitro antibacterial activity of squaw mint (Mentha pulegium L., Lamiaceae family), catnip (Nepeta cataria L., lamiaceae), lemon balm (Melissa officinalis L.,Lamiaceae) and peppermint oil for mastitis treatment was evaluated.
Abstract: Abstract Background Mastitis in dairy cattle is a highly prevalent infectious disease, caused by various pathogens, mainly Staphylococcu aureus and Escherichia Coli, considerable economic loss worldwide. Objectives The aim of this study was to evaluate the in vitro activity of Herbal plants used against S. aureus and E. coli bacteria which are the causative agents of mastitis. Methods Therefore, in this study we investigate the antimicrobial effect of plant to evaluate the in vitro antibacterial activity of squaw mint (Mentha pulegium L., Lamiaceae family), catnip (Nepeta cataria L., Lamiaceae), lemon balm (Melissa officinalis L., Lamiaceae), for mastitis treatment. Solutions prepared in fixed oils, against S. aureus and E. coli bacteria which are the main agents of mastitis. Isolation and antibiotic susceptibility analyses of milk samples taken from 100 subclinical mastitis dairy cows were performed. The antibacterial properties of the solutions were analysed by a disk diffusion method. Results In the bacterial isolation, S. aureus was determined 97.7% and E. coli 53.5% positive of cows with mastitis. Antibacterial susceptibility test of the Lemon balm extract and essential oil showed maximum zone of inhibition against S. aureus 30 µl (23 mm), followed by 20 µl (19 mm), E. coli (19 mm) and 10 µl (5–7 mm), of the same extract against the Gram‐positive bacteria. The ethanol extracts show the similar activity against the Gram‐negative bacteria at 30, 20, and 10 µl (18–20 mm). Followed by S. aureus, when the zone areas for the susceptible solutions (Lemon balm, and essential oil) and the control group were compared, determined that there was little difference between for S. aureus and E. coli. Conclusions This study hence indicated that in vitro cultured plantlets of lemon balm and peppermint oil can be used as the alternative method for production of mastitis and cheap source its precursor with antimicrobial activities.
TL;DR: In this paper , the anti-fibrotic effect of ALS-L1023 was investigated in the non-alcoholic fatty liver disease (NAFLD) model.
Abstract: ALS-L1023 is an ingredient extracted from Melissa officinalis L. (Labiatae; lemon balm), which is known as a natural medicine that suppresses angiogenesis. Herein, we aimed to determine whether ALS-L1023 could alleviate liver fibrosis in the non-alcoholic fatty liver disease (NAFLD) model. C57BL/6 wild-type male mice (age, 6 weeks old) were fed a choline-deficient high-fat diet (CDHFD) for 10 weeks to induce NAFLD. For the next 10 weeks, two groups of mice received the test drug along with CDHFD. Two doses (a low dose, 800 mg/kg/day; and a high dose, 1200 mg/kg/day) of ALS-L1023 were selected and mixed with feed for administration. Obeticholic acid (OCA; 10 mg/kg/day) was used as the positive control. Biochemical analysis revealed that the ALS-L1023 low-dose group had significantly decreased alanine transaminase and aspartate transaminase. The area of fibrosis significantly decreased due to the administration of ALS-L1023, and the anti-fibrotic effect of ALS-L1023 was greater than that of OCA. RNA sequencing revealed that the responder group had lower expression of genes related to the hedgehog-signaling pathway than the non-responder group. ALS-L1023 may exert anti-fibrotic effects in the NAFLD model, suggesting that it may provide potential benefits for the treatment of liver fibrosis.
TL;DR: In this paper , a phytochemical and biological screening of medicinal and aromatic plants from the Lamiaceae family (Origanum vulgare L, Thymus vulgaris L, Ocimum basilicum L, Salvia officinalis L.
TL;DR: In this paper , the impact of essential oils of Ocimum basilicum, Eugenia caryophyllata Thumb, Hyssopus officinalis L., Coriandrum sativum, Nepeta cataria L., Nepeta transcaucasica, Lavandula angustifolia Moench, Laurus nobilis L, Mentha longifólia L, 4 sorts of Mentha piperita L, Abies alba Mill, Artemisia taurica Willd., Rosa gallica L., Pinus silvestris L., Thymus vulgaris L.
Abstract: Has been investigated impact of essential oils of Ocimum basilicum L., Eugenia caryophyllata Thumb., Hyssopus officinalis L., Coriandrum sativum L., Nepeta cataria L., Nepeta transcaucasica, Lavandula angustifolia Moench., Laurus nobilis L., Mentha longifólia L., 4 sorts of Mentha piperita L, Abies alba Mill., Artemisia taurica Willd., Rosa gallica L., Pinus silvestris L., Thymus vulgaris L., Satureja montana L., Satureja hortensis L. on psycho-emotional state of women at age from 50 to 85 years, who were divided into control and experimental groups. Control group - psych relax during 10, 20 or 30 minutes, experimental groups - the same psych relax + inhalation by essential oils at concentration in air 1 mg/m 3 . Before and after procedure was measured parameters of anxiety, depression and other parameters of psych emotional state using hospital scale of anxiety and depression and method of investigation of self-estimation by Dembo-Rubinstein in original modification. It is shown there are essential oils with anxiolytic, antidepressant activity and make better psych emotional state.