Showing papers on "Mevalonic acid published in 1974"
TL;DR: A rapid, highly reproducible, micro-incubation, direct thin-layer chromatographic assay for 3-hydroxy-3-methylglutaryl-CoA reductase is described, which accurately determines the rapid inactivation of reduct enzyme by MgATP and the low levels of reduCTase activity in cholesterol-treated L cells are accurately determined.
398 citations
TL;DR: Procedures were developed for the determination of the activity of the microsomal enzyme 3-hydroxy-3-methylglutaryl CoA reductase in human liver, applicable to specimens obtained by percutaneous liver biopsy.
101 citations
TL;DR: It is demonstrated that the stimulation of the reductase activity in hypophysectomized-diabetic rats requires the mediation of both insulin and l-triiodothyronine and neither hormone alone is effective.
47 citations
TL;DR: The results demonstrate that the renal cortex is the primary site of mevalonic acid metabolism within the kidney and that the glomerulus is responsible for 95% of the mevalonate metabolized by the renal cerebral cortex.
37 citations
TL;DR: Incubation of a cell-free system from immature seeds of Cucurbita maxima Duch yielded the C19-gibberellin, [14C]GA4, in addition to the C20-gigorberellins, [ 14C)GA37, [13C] GA13 and [14 C]GA43.
Abstract: Incubation of a cell-free system from immature seeds of Cucurbita maxima Duch. with [(14)C]GA12-aldehyde derived from [(14)C]mevalonic acid in the same system yielded the C19-gibberellin, [(14)C]GA4, in addition to the C20-gibberellins, [(14)C]GA37, [(14)C]GA13 and [(14)C]GA43. (GA-gibberellin).
35 citations
TL;DR: The hamster liver lipid contained a component which could be labelled with mannose and mevalonic acid, and the retinol labelled compound had similar properties to in vitro prepared mannosyl-retinyl-phosphate.
28 citations
TL;DR: The 13C n.m.r. signals of the trichothecanes have been assigned as mentioned in this paper, indicating that farnesyl pyrophosphate is folded in the manner (3).
Abstract: The 13C n.m.r. signals of the trichothecanes have been assigned. [2-13C]Mevalonic acid has been prepared and incorporated into trichothecolone. C-4, C-14, and C-8 were enriched indicating that farnesyl pyrophosphate is folded in the manner (3).
24 citations
TL;DR: The formation of phytol from [2-14 C]mevalonate but not from [1- 14 C]isopentenyl pyrophosphate was found to be inhibited by phytyl pyroph phosphate.
23 citations
TL;DR: U-14C-L-Leucine and 4,5-3H-L leucine were incorporated into linalool (I) in Cinnamomum Camphora Sieb. var. Linalooliferum Fujita in 0.004% yield at the highest value as mentioned in this paper.
Abstract: U-14C-L-Leucine and 4,5-3H-L-leucine were incorporated into linalool (I) in Cinnamomum Camphora Sieb. var. linalooliferum Fujita in 0.004% yield at the highest value. Its labeling patterns have indicated that DMAPP may originate not only from mevalonic acid but also from leucine not via the acid.
18 citations
TL;DR: Two enzymes of polyisoprenoid synthesis, 3-hydroxy-3-methylglutaryl coenzyme A (HMG CoA) reductase and mevalonate kinase (ATP:mevalonates 5-phosphotransferase, EC 2.7.1.36), are present in the microsomal and soluble fractions of Neurospora crassa, respectively.
16 citations
TL;DR: Incorporation experiments using sodium [2-14C]-, [ 2-3H]-, (3R)-[5- 14C]- and [2]-mevalonates and with mevalonate stereospecifically tritiated at C(2) demonstrate the transformation of mevalonic acid (8) into verrucarinic acid (5) Degradation experiments showed that this transformation occurs with a hydrogen 1, 2-shift of the ‘pro-2R’ hydrogen atom of meavalonate to C(3) of
Abstract: Incorporation experiments using sodium [2-14C]-, [2-3H]-, (3R)-[5-14C]- and [2-3H, 2-14C]-mevalonates and with mevalonates stereospecifically tritiated at C(2) demonstrate the transformation of mevalonic acid (8) into verrucarinic acid (5) Degradation experiments showed that this transformation occurs with a hydrogen 1, 2-shift of the ‘pro-2R’ hydrogen atom of mevalonate to C(3) of verrucarinate A possible mechanistic pathway is discussed
TL;DR: The Pinus pinaster seedlings contain mevalonate kinase which phosphorylates mevalonic acid (MVA) as discussed by the authors, which is a kinase kinase that phosphorylated mevalony kinase.
TL;DR: The biosynthesis of lophocerine (1,2,3,4,tetrahydro-1-isobutyl-6-methoxy-2-methylisoquinolin-7-ol) in intact Lophocereus schottii plants has been studied as discussed by the authors.
Abstract: The biosynthesis of lophocerine (1,2,3,4-tetrahydro-1-isobutyl-6-methoxy-2-methylisoquinolin-7-ol) in intact Lophocereus schottii plants has been studied. The C5 unit of the alkaloid probably arises independently from both leucine and mevalonic acid. 3-Methylbut-3-enyl pyrophosphate, 3-methylbutan-1-ol, and 3-methylbutanal have been shown to be intermediates in the biosynthesis.
TL;DR: In the aerial parts the uptake of radioactivity was rapid in the cycloartenol and lipidphytosterol fractions, but less so in the glucoside phytosterols, but in the roots the glucose incorporation of label was considerably more rapid than in the lipid fraction.
Abstract: d. l. [2-14C] mevalonic acid was fed to Digitalis purpurea flowering plants and the 4-4′-dimethyl and phytosterol fractions isolated at time intervals from the glucoside and lipid portions of the plant extract. The incorporation of mevalonic acid was determined in both the aerial parts and the roots over a period of 35 days. In the aerial parts the uptake of radioactivity was rapid in the cycloartenol and lipid phytosterol fractions, but less so in the glucoside phytosterols. In the roots the glucoside phytosterols incorporation of label was considerably more rapid than in the lipid fraction.
TL;DR: Evidence is provided to show that enhanced sterol biosynthesis is due to an increased formation of mevalonic acid, indicating that the primary site of action of the chemical is on one or more steps in the pathway from acetate to mevalonate.
TL;DR: Although the conversion of labeled mevalonate to cholesterol was only slightly influenced, the detergents markedly affected the turnover of squalene and methyl sterol precursors in the presence of Tween 80 or Triton X-100.
TL;DR: Two compounds inhibited the conversion of [1-(14)C]isopentenyl pyrophosphate into cholesterol and the synthesis of cholesterol and fatty acids from [2-(14]C]acetate and caused stimulation of acetate incorporation into fatty acids at low concentrations.
Abstract: 2,4-Dichlorophenoxyacetic acid and 2,4,5-trichlorophenoxyacetic acid inhibited the incorporation of [2-(14)C]mevalonate into cholesterol and non-saponifiable lipids. Both compounds inhibited the conversion of [1-(14)C]isopentenyl pyrophosphate into cholesterol and the synthesis of cholesterol and fatty acids from [2-(14)C]acetate. There was no inhibition of the conversion of [1-(14)C]mevalonate into CO(2). At low concentrations (0.5mm) of the compounds there was a stimulation of acetate incorporation into fatty acids.
TL;DR: Incubation of (2R)- and (2S)-MVA with a cell free homogenate of yeast resulted in sterols in 5α-cholesta-7,24-dien-3β-ol rather than in C 28 sterols.
TL;DR: In tigogenin [(25R)-5α-spirostan-3β-ol] biosynthesized in Digitalis lanata a 4-pro-R-proton of mevalonic acid occupies the 24- Pro-S-position, which indicates that reduction of Δ24-biosynthetic intermediates occurs with trans-stereochemistry.
Abstract: In tigogenin [(25R)-5α-spirostan-3β-ol] biosynthesized in Digitalis lanata a 4-pro-R-proton of mevalonic acid occupies the 24-pro-S-position, which indicates that reduction of Δ24-biosynthetic intermediates occurs with trans-stereochemistry.
TL;DR: In this paper, it was shown that the 26-carbon in sarsasapogenin biosynthesized in Agave attenuata Solm is derived from C-2 of mevalonic acid.
Abstract: It is shown that the 26-carbon in sarsasapogenin biosynthesized in Agave attenuata Solm. is derived from C-2 of mevalonic acid.
TL;DR: The activities are maximal at pH 7 and the formation of mevalonic acid pyrophosphate and isopentenyl-pyroph phosphate reaches a maximal level after an incubation time of 180 min whereas the level of meValonic acid phosphate begins to decrease after 90 min.
TL;DR: The localization of total and newly synthesized [3H]prenol in Lactobacillus casei was investigated by autoradiography of electron micrographs of bacteria grown with [3 H]mevalonic acid; the distribution of radioactive grains differed from a random distribution in being concentrated in the membrane.
Abstract: The localization of total and newly synthesized [3H]prenol in Lactobacillus casei was investigated by autoradiography of electron micrographs of bacteria grown with [3H]mevalonic acid. The distribution of radioactive grains differed from a random distribution in being concentrated in the membrane; this confirmed earlier chemical studies. The distribution of total [3H]prenol along the cell, after continuous growth with [3H]mevalonic acid, did not indicate any specific localization. Newly synthesized prenol, located after a 20 min pulse with [3H]mevalonic acid, was also uniformly distributed except in organisms which had spent the previous 20 min forming septa. In these bacteria there was a concentration of prenol in the septal region.
TL;DR: In this article, the synthesis in vivo of linalool and 4-terpineol from mevalonic acid (MVA) by Pinus pinaster seedlings has been demonstrated.