Showing papers in "Biochemical and Biophysical Research Communications in 1974"

TL;DR: The structural framework of the rat liver nucleus has been identified and consists of a nuclear protein matrix that is composed primarily of three acidic polypeptide fractions in the molecular weight range of 60–70,000 daltons.

TL;DR: Further structural simplifications of the active molecule are reported; natural N-acetyl-muramyl-tripeptides have been found active and the smallest adjuvant molecule found is a synthetic N- acetyl muramyl -dipeptide prepared by Sinay et al.

TL;DR: It is suggested that paraquat toxicity may be mediated through the transfer of a single electron from reduced paraqu at to oxygen and thus form superoxide ion.

TL;DR: Positive and negative ion mass spectra are presented for the amino acids arginine and cystine which are characterized by intense quasi-molecular ions.

TL;DR: Cytochrome P-450 was purified from liver microsomes of phenobarbital-pretreated rabbits to a specific content of 16 to 17 nmoles per mg of protein with a yield of about 10 %.

TL;DR: In yeast tunicamycin acts primarily as an inhibitor of glycoprotein synthesis and not of general glucosamine metabolism, and incorporation of 3H-glucosamine into trichloroacetic acid-insoluble products was only partially reduced.

TL;DR: The results suggest that uptake into fibroblasts requires a specific marker on the enzyme, and the uptake of the normal fibroblast hydrolase can be abolished by periodate oxidation under conditions that do not affect its catalytic activity, stability, or binding to Concanavalin A — Sepharose.

TL;DR: The receptor for acetylcholine purified from electric tissue of Torpedo californica has been assayed both by affinity-alkylation and by neurotoxin binding, resulting in the exclusive labeling of the 39,000 dalton subunit.

TL;DR: The binding of [ 3 H]-alprenolol (a potent β-adrenergic antagonist) to sites in frog erythrocyte membranes has been studied by a centrifugal assay as discussed by the authors.

TL;DR: The data indicate that approximately 60% of the nucleic acid adducts were derived from reactions in vivo with aflatoxin B1-2,3-oxide, which is a probable ultimate carcinogenic metabolite.

TL;DR: Guinea Pig lung homogenates as well as intact guinea pig lung converted added arachidonic acid predominantly into PHD and HHT, metabolites of the prostaglandin endoperoxide PGG2, and to a lesser extent into the classical prostag landins PGE2 and PGF2α.

TL;DR: Spermatozoa extracted with Triton X-100 solutions containing 0.005 M CaCl2 have nearly symmetrical flagellar beat patterns and straight swimming paths when reactivated in ATP solutions containing low concentrations of free calcium ion.

TL;DR: Cell free extracts prepared from resting yeast were shown to contain a cryptic trehalase, which under physiological conditions could be activated by cyclic 3′,5′-AMP to the same degree as in vivo, suggesting that in the lag phase of growth, the level of trehalose in baker's yeast is under control of a system, regulated by thelevel of cyclic 2′,3′,4″-AMP.

TL;DR: It is demonstrated that the antibody binds preferentially to the liver microsomes isolated from phenobarbital-pretreated rats, in which the 45,000-dalton hemoprotein has been shown to be induced.

TL;DR: The active fractions were capable of enzymatically supporting the peroxidation of isolated mitochondria in the presence of organically complexed Fe+3 and NADPH, and in this respect the specific activity was found to be about ten times higher than in microsomes.

TL;DR: Conversion proceeds at one-fifth the rate obtained with ATP though the extent of reaction and final level of activation of the enzyme are the same, however, the thiophosphorylase a produced is resistant to phosphoryLase phosphatase and, therefore, behaves as a competitive inhibitor with a KI of 3 μM, similar to the KM obtained with normal phosphoryl enzyme a.

TL;DR: Cumene hydroperoxide is capable of supporting the aromatic hydroxylation of a variety of compounds in the presence of hepatic microsomes and it is suggested that the oxenoid species of cytochrome P-450 involved in microsomal aromatic hydoxylation is present in a form equivalent to the ferryl from.

TL;DR: It appears that sperm are quiescent on the male because they lack cAMP, and that calcium, supplied at ejaculation, initiates rapid cAMP synthesis to produce motility.

TL;DR: From a single preparation of proteinyl-14C-tyrosine variable proportions of dimer and higher molecular weight aggregates were obtained by incubating in different conditions.

TL;DR: NADPH-cytochrome P-450 reductase was highly purified from liver microsomes of phenobarbital-induced rats by column chromatography on DEAE-cellulose,DEAE-Sephadex A-50, and hydroxylapatite in the presence of deoxycholate or Renex 690, a nonionic detergent.

TL;DR: Electrophoresis on sodium dodecylsulfate-containing polyacrylamide gels of high density lipoprotein obtained from plasma of sucrose-fed rats reveals a complex pattern of three major bands and several minor, low molecular weight bands.

TL;DR: A mechanism is proposed that relates superoxide anion generation to the univalent reduction of O 2 by the activated NADPH (and NADH) oxidase in response to superoxide dismutase inhibition of nitroblue tetrazolium reduction from intact human polymorphonuclear leukocytes.

TL;DR: ThePurification and characteristics of this enzyme are described and the evolutionary implications of the presence in procaryotes of this kind of superoxide dismutase (formerly characteristic of eucaryotes) are discussed.

TL;DR: A high-affinity form of ribulose diphosphate carboxylase, observed transiently in spinach-leaf extracts soon after extraction, was inhibited by O 2 competitively with respect to CO 2.

TL;DR: The reconstituted system containing purified cytochrome P-450, reductase, and phosphatidylcholine catalyzed the hydroxylation of benzphetamine, cyclohexane, aniline, and laurate.

TL;DR: The divalent ionophores A23187 and X-537A induce parthenogenesis in sea urchin eggs from their ability to mobilize intracellular Ca 2+ , which is implicated in both artificial parthenogenic as well as the natural fertilization process.

TL;DR: A new synthesis of an R-factor-specific protein occurs in E. coli minicells containing tetracycline-resistant R factors when they are incubated in the presence of tetrACYcline.

TL;DR: A microsomal Mg-ATPase from dog gastric mucosa can be stimulated by gramicidin, nigericin and valinomycin in a KCl medium and the data are consistent with the ATP hydrolysis driven entry of H+ ions across the vesicle membrane.

TL;DR: The data indicate that the antitumor activities of adriamycin could possibly be partly due to inhibition of CoQ 10 -enzymes in electron transfer processes of cell respiration in addition to intercalation within DNA helices.

TL;DR: Three different molecular species of collagen and a soluble form of elastin were obtained by digestion of human aortas with pepsin, and appear to represent type IV collagen previously described in basement membranes and type III collagen, recently found in fetal skin.