Showing papers on "Upstream activating sequence published in 1981"
TL;DR: It appears that the sequences between 16 and 32 nucleotides upstream from the 5' terminus of the tk gene are required to specify the exact start site of transcription, however, control of both the efficiency of transcription and rough specification of the position of initiation depends on sequences 40--100 nucleotide upstream fromthe tk structural gene.
495 citations
TL;DR: Results indicate that the region of the genome upstream of the cap site, with boundaries within 15-17 nucleotides to either side of the T-A-T-A -A- a-a-A sequence, is required for the initiation of transcription in vitro from the major late promoter of adenovirus 2.
Abstract: We have identified a region of the viral genome required for the initiation of transcription in vitro from the major late promoter of adenovirus 2. A fragment of the adenovirus genome containing the cap site of the major late transcripts was inserted into plasmid pBR322 and cloned. Deletions were then generated in vitro in and around the T-A-T-A-A-A-A sequence located 25-31 nucleotides (positions -25 to -31) upstream from the cap site. DNAs with these deletions were tested for their ability to initiate transcription in vitro by the method of Manley et al. [Manley, J. L., Fire, A., Cano, A., Sharp, P. A. & Gefter, M. L. (1980) Proc. Natl. Acad. Sci. USA 77, 3855-3859]. Whereas removal of sequences upstream from position -47 or downstream from position -12 did not abolish transcription, deletions extending into, or beyond, the T-A-T-A-A-A-A sequence reduced transcription to less than 1/10th. Removal of the normal cap site slightly reduced, but did not abolish, transcription. These results indicate that the region of the genome upstream of the cap site, with boundaries within 15-17 nucleotides to either side of the T-A-T-A-A-A-A sequence, is required for the initiation of transcription in vitro from the major late promoter of adenovirus 2.
165 citations
TL;DR: The nucleotide sequence of the promoter for aB.
92 citations
TL;DR: The results suggest that regions outside of the sequence directly recognized by a regulatory protein can affect genetic expression from the site.
36 citations
01 Jan 1981
TL;DR: In this article, the effect of modifications introduced into the 5′-flanking region of the rabbit β-globin gene on correct transcription was determined in vivo and in vitro, and a region 57 to 84 bp upstream of the cap site, containing the CAAT box, had a potentiating effect on the level of transcription in vivo but was not required for correct initiation.
Abstract: . The effect of modifications introduced into the 5′-flanking region of the rabbit β-globin gene on correct transcription was determined in vivo and in vitro. The ATA box sequence located 25 to 31 bp upstream of the cap site was required for detectable levels of transcription; moreover, it programmed the starting points of β-globin transcripts. A region 57 to 84 bp upstream of the cap site, containing the CAAT box, had a potentiating effect on the level of transcription in vivo but was not required for correct initiation.
10 citations