A
Alfred M. Handler
Researcher at Agricultural Research Service
Publications - 88
Citations - 3887
Alfred M. Handler is an academic researcher from Agricultural Research Service. The author has contributed to research in topics: Anastrepha suspensa & Gene. The author has an hindex of 30, co-authored 87 publications receiving 3498 citations. Previous affiliations of Alfred M. Handler include United States Department of Agriculture.
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Journal ArticleDOI
piggyBac is a flexible and highly active transposon as compared to Sleeping Beauty, Tol2, and Mos1 in mammalian cells
Sareina Chiung Yuan Wu,Yaa-Jyuhn James Meir,Craig J. Coates,Craig J. Coates,Alfred M. Handler,Pawel Pelczar,Stefan Moisyadi,Stefan Moisyadi,Joseph M. Kaminski +8 more
TL;DR: The high transposition activity of piggyBac and the flexibility for molecular modification of its transposase suggest the possibility of using it routinely for mammalian transgenesis.
Journal ArticleDOI
Germline transformation of Drosophila melanogaster with the piggyBac transposon vector
TL;DR: PiggyBac transformation in two distantly related dipteran species and efficient expression of the gfp marker supports the potential use of this system in other dipterans, and perhaps insects in general.
Journal ArticleDOI
The lepidopteran transposon vector, piggyBac, mediates germ-line transformation in the Mediterranean fruit fly
TL;DR: The efficient and stable transformation of the medfly with a lepidopteran vector represents transposon function over a relatively large evolutionary distance and suggests that the piggyBac system will be functional in a broad range of insects.
Journal ArticleDOI
Use of the piggyBac transposon for germ-line transformation of insects
TL;DR: Germ-line transformation of insects is now possible with four independent transposable element vector systems, and it appears that piggyBac has recently traversed insect orders by horizontal transmission, possibly mediated by a baculovirus or other viral system.
Journal ArticleDOI
Gene Content Evolution in the Arthropods
Gregg W.C. Thomas,Elias Dohmen,Elias Dohmen,Daniel S.T. Hughes,Daniel S.T. Hughes,Shwetha C. Murali,Shwetha C. Murali,Monica F. Poelchau,Karl M. Glastad,Karl M. Glastad,Clare A. Anstead,Nadia A. Ayoub,Philip J. Batterham,Michelle Bellair,Greta J. Binford,Hsiao-Tuan Chao,Yolanda H. Chen,Christopher P. Childers,Huyen Dinh,Harshavardhan Doddapaneni,Jian J. Duan,Shannon Dugan,Lauren A. Esposito,Markus Friedrich,Jessica E. Garb,Robin B. Gasser,Michael A. D. Goodisman,Dawn E. Gundersen-Rindal,Yi Han,Alfred M. Handler,Masatsugu Hatakeyama,Lars Hering,Wayne B. Hunter,Panagiotis Ioannidis,Panagiotis Ioannidis,Joy Jayaseelan,Divya Kalra,Abderrahman Khila,Pasi K. Korhonen,Carol Eunmi Lee,Sandra L. Lee,Yiyuan Li,Amelia R.I. Lindsey,Amelia R.I. Lindsey,Georg Mayer,Alistair P. McGregor,Duane D. McKenna,Bernhard Misof,Mala Munidasa,Monica Munoz-Torres,Donna M. Muzny,Oliver Niehuis,Nkechinyere Osuji-Lacy,Subba Reddy Palli,Kristen A. Panfilio,Matthias Pechmann,Trent Perry,Ralph S. Peters,Helen C. Poynton,Nikola-Michael Prpic,Jiaxin Qu,Dorith Rotenberg,Coby Schal,Sean D. Schoville,Erin D. Scully,Evette Skinner,Daniel B. Sloan,Richard Stouthamer,Michael R. Strand,Nikolaus U. Szucsich,Asela J. Wijeratne,Asela J. Wijeratne,Neil D. Young,Eduardo E. Zattara,Joshua B. Benoit,Evgeny M. Zdobnov,Michael E. Pfrender,Kevin J. Hackett,John H. Werren,Kim C. Worley,Richard A. Gibbs,Ariel D. Chipman,Robert M. Waterhouse,Erich Bornberg-Bauer,Erich Bornberg-Bauer,Erich Bornberg-Bauer,Matthew W. Hahn,Stephen Richards,Stephen Richards +88 more
TL;DR: These analyses demonstrate how large-scale comparative genomics can provide broad new insights into the genotype to phenotype map and generate testable hypotheses about the evolution of animal diversity.