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Andrew L. Markhard
Researcher at Howard Hughes Medical Institute
Publications - 6
Citations - 3042
Andrew L. Markhard is an academic researcher from Howard Hughes Medical Institute. The author has contributed to research in topics: mTORC2 & Uniporter. The author has an hindex of 5, co-authored 6 publications receiving 2805 citations. Previous affiliations of Andrew L. Markhard include Massachusetts Institute of Technology & Broad Institute.
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Journal ArticleDOI
Prolonged rapamycin treatment inhibits mTORC2 assembly and Akt/PKB.
Dos D. Sarbassov,Siraj M. Ali,Siraj M. Ali,Shomit Sengupta,Shomit Sengupta,Joon Ho Sheen,Joon Ho Sheen,Peggy P. Hsu,Peggy P. Hsu,Alex F. Bagley,Alex F. Bagley,Andrew L. Markhard,Andrew L. Markhard,David M. Sabatini,David M. Sabatini +14 more
TL;DR: It is shown that rapamycin inhibits the assembly of mTORC2 and that, in many cell types, prolongedRapamycin treatment reduces the levels of m TORC2 below those needed to maintain Akt/PKB signaling.
Journal ArticleDOI
Architecture of the mitochondrial calcium uniporter
Kirill Oxenoid,Ying Dong,Chan Cao,Chan Cao,Tanxing Cui,Yasemin Sancak,Andrew L. Markhard,Zenon Grabarek,Liangliang Kong,Zhijun Liu,Bo OuYang,Yao Cong,Vamsi K. Mootha,James J. Chou,James J. Chou +14 more
TL;DR: The structure of the pore domain of MCU from Caenorhabditis elegans is determined using nuclear magnetic resonance (NMR) and electron microscopy, and this is one of the largest membrane protein structures characterized by NMR, and provides a structural blueprint for understanding the function of this channel.
Journal ArticleDOI
Genetic Screen for Cell Fitness in High or Low Oxygen Highlights Mitochondrial and Lipid Metabolism.
Isha H. Jain,Isha H. Jain,Sarah E. Calvo,Sarah E. Calvo,Andrew L. Markhard,Andrew L. Markhard,Owen S. Skinner,Owen S. Skinner,Tsz-Leung To,Tsz-Leung To,Tslil Ast,Tslil Ast,Vamsi K. Mootha,Vamsi K. Mootha +13 more
TL;DR: This resource performs genome-wide CRISPR growth screens at 21%, 5%, and 1% oxygen to systematically identify gene knockouts with relative fitness defects in high oxygen or low oxygen, and links hundreds of genes to oxygen homeostasis.
Book ChapterDOI
Development of ATP-competitive mTOR inhibitors.
Qingsong Liu,Seong A. Kang,Carson C. Thoreen,Wooyoung Hur,Jinhua Wang,Jae Won Chang,Andrew L. Markhard,Jianming Zhang,Taebo Sim,David M. Sabatini,Nathanael S. Gray +10 more
TL;DR: The experimental approaches to develop Torin1 using a medium throughput cell-based screening assay and structure-guided drug design are described, which aim to develop ATP-competitive mTOR inhibitors that would block both mTORC1 and m TORC2 complex activity.
Journal ArticleDOI
Reduced dosage of the chromosome axis factor Red1 selectively disrupts the meiotic recombination checkpoint in Saccharomyces cerevisiae.
Tovah E. Markowitz,Daniel Suarez,Hannah G. Blitzblau,Neem J. Patel,Andrew L. Markhard,Amy J. MacQueen,Andreas Hochwagen,Andreas Hochwagen +7 more
TL;DR: Separable roles for Red1 are indicated in building the structural axis of meiotic chromosomes and mounting a sustained recombination checkpoint response in S. cerevisiae using the putative condensin allele ycs4S.