A
Arthur Sedivy
Publications - 4
Citations - 45
Arthur Sedivy is an academic researcher. The author has contributed to research in topics: Microscale thermophoresis & Ligand (biochemistry). The author has an hindex of 3, co-authored 4 publications receiving 17 citations.
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Microscale Thermophoresis and additional effects measured in NanoTemper Monolith instruments.
TL;DR: In this paper, the authors present the experience gathered in scientific service facilities about this technique and make scientists aware of possible pitfalls with the intention to promote knowledge and good practice throughout the scientific community.
Journal ArticleDOI
Reproducibility and accuracy of microscale thermophoresis in the NanoTemper Monolith: a multi laboratory benchmark study
Blanca López-Méndez,Bruno Baron,Chad A. Brautigam,Thomas A. Jowitt,Stefan H. Knauer,Stephan Uebel,Mark A. Williams,Arthur Sedivy,Olga Abian,Celeste Abreu,Malgorzata Adamczyk,Wojciech Bal,Sylvie Berger,Alexander K. Buell,Carlo Carolis,Tina Daviter,Alexander Fish,Maria Garcia-Alai,Christian Guenther,Josef Hamacek,Jitka Holková,Josef Houser,Christopher M. Johnson,Sharon M. Kelly,Andrew P. Leech,Caroline Mas,Daumantas Matulis,Stephen H. McLaughlin,Roland Montserret,Rouba Nasreddine,Reine Nehmé,Quyen Nguyen,David Ortega-Alarcon,Kathryn Perez,Katja Pirc,Grzegorz Piszczek,Marjetka Podobnik,Natalia Rodrigo,Jasmina Rokov-Plavec,Susanne Schaefer,Timothy Sharpe,June Southall,David Staunton,Pedro Tavares,Ondrej Vanek,Michael Weyand,Di Wu +46 more
TL;DR: This study characterized the instrument properties and evaluated instrument performance, reproducibility, the effect of different analysis tools, the influence of the experimenter during data analysis, and thus the overall reliability of this method.
Journal ArticleDOI
Natural Killer Cell Activation Receptor NKp30 Oligomerization Depends on Its N-Glycosylation.
Ondřej Skořepa,Samuel Pazicky,Barbora Kalousková,Jan Bláha,Celeste Abreu,Tomáš Ječmen,Michal Rosůlek,Michal Rosůlek,Alexander Fish,Arthur Sedivy,Karl Harlos,Jan Dohnálek,Tereza Skálová,Ondřej Vaněk +13 more
TL;DR: New insights are provided into the structural basis ofNKp30 oligomerization and how the stalk region and glycosylation of NKp30 affect its ligand affinity are explained, which furthers the understanding of the molecular mechanisms involved in NK cell activation.
Journal ArticleDOI
Standard operating procedure for NanoTemper Monolith measurements.
TL;DR: In this article, a step-by-step standard operating procedure (SOP) about how to properly conduct a microscale thermophoresis (MST), also known as temperature-related intensity change (TRIC) experiment using the NanoTemper Technology GmbH Monolith instruments is given.