B. de Kruijff

TL;DR: The precursor of the chloroplast protein ferredoxin from Silene pratensis was expressed in Escherichia coli and the preferredoxin level was low, and the protein was soluble, but the first methionine residue of the expected sequence was absent in E. coli.

TL;DR: In this in vitro system SecB and not trigger factor could stabilize a translocation-competent state of prePhoE, and translocation competency of proOmpA could be induced by both trigger factor and SecB protein, suggesting specificity in interactions between cytosolic factors and precursors in outer membrane protein translocation.

TL;DR: The present data show the critical importance of the anionic lipid content in monomolecular layers for the interaction with PhoE signal peptide, which correlates with the reduced translocation competence of Escherichia coli strain HD3122.

TL;DR: In this structure a rapid transbilayer movement of phosphatidylcholine and a highly increased permeability towards Mn2+ are observed.

TL;DR: Binding experiments showed that fragments of apocytochrome c with the highest net positive charge have the highest affinity for negatively charged large unilamellar phosphatidylserine vesicles, and Implications of these data for the import of apoc tochrome c into mitochondria will be discussed.