B
Barbara Varnum-Finney
Researcher at Fred Hutchinson Cancer Research Center
Publications - 37
Citations - 4186
Barbara Varnum-Finney is an academic researcher from Fred Hutchinson Cancer Research Center. The author has contributed to research in topics: Stem cell & Notch signaling pathway. The author has an hindex of 22, co-authored 34 publications receiving 3957 citations. Previous affiliations of Barbara Varnum-Finney include ImClone Systems.
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Journal ArticleDOI
Pluripotent, cytokine-dependent, hematopoietic stem cells are immortalized by constitutive Notch1 signaling.
Barbara Varnum-Finney,Lanwei Xu,Carolyn Brashem-Stein,Cynthia Nourigat,David A. Flowers,Sonia Bakkour,Warren S. Pear,Irwin D. Bernstein,Irwin D. Bernstein +8 more
TL;DR: It is demonstrated that constitutive Notch1 signaling in hematopoietic cells established immortalized, cytokine-dependent cell lines that generated progeny with either lymphoid or myeloid characteristics both in vitro and in vivo.
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Endothelial Cells Are Essential for the Self-Renewal and Repopulation of Notch-Dependent Hematopoietic Stem Cells
Jason M. Butler,Daniel J. Nolan,Eva L. Vertes,Barbara Varnum-Finney,Hideki Kobayashi,Andrea T. Hooper,Marco Seandel,Marco Seandel,Koji Shido,Ian A. White,Mariko Kobayashi,Larry Witte,Chad May,Carrie J. Shawber,Yuki Kimura,Jan Kitajewski,Zev Rosenwaks,Irwin D. Bernstein,Shahin Rafii +18 more
TL;DR: Angiogenic models are developed to demonstrate that EC-derived angiocrine growth factors support in vitro self-renewal and in vivo repopulation of authentic LT-HSCs, and establish an instructive vascular niche for clinical-scale expansion of LT- HSCs and a cellular platform to identify stem cell-active trophogens.
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The Notch ligand, Jagged-1, influences the development of primitive hematopoietic precursor cells
Barbara Varnum-Finney,Louise E. Purton,Monica Yu,Carolyn Brashem-Stein,David A. Flowers,Steven J. Staats,Kateri A. Moore,Isabelle Le Roux,Robert S. Mann,Grace E. Gray,Spyros Artavanis-Tsakonas,Irwin D. Bernstein +11 more
TL;DR: It is found that Jagged-1, presented both on the cell surface and on beads, promoted a twofold to threefold increase in the formation of primitive precursor cell populations in vitro.
Journal ArticleDOI
Immobilization of Notch ligand, Delta-1, is required for induction of notch signaling.
Barbara Varnum-Finney,Lizi Wu,Monica Yu,Carolyn Brashem-Stein,Steven J. Staats,David A. Flowers,James D. Griffin,Irwin D. Bernstein +7 more
TL;DR: It is indicated that Delta-1 extracellular domain must be immobilized to induce Notch activation in C2 or U20S cells and that non-immobilized Delta- 1 extracllular domain is inhibitory to Notch function.
Journal ArticleDOI
Hematopoietic Stem Cell Function and Survival Depend on c-Myc and N-Myc Activity
Elisa Laurenti,Barbara Varnum-Finney,Anne Wilson,Isabel Ferrero,William Blanco-Bose,Armin Ehninger,Paul S. Knoepfler,Pei Feng Cheng,H. Robson MacDonald,Robert N. Eisenman,Irwin D. Bernstein,Andreas Trumpp +11 more
TL;DR: While most dKO hematopoietic cells undergo apoptosis, only self-renewing HSCs accumulate the cytotoxic molecule Granzyme B, normally employed by the innate immune system, thereby revealing an unexpected mechanism of stem cell apoptosis.