Showing papers by "Byung Sun Min published in 2010"
TL;DR: The results demonstrate that the α‐pyrone ring of coumarins significantly enhanced the capacity of inhibiting oxidative reactions of Coumarins, and contributed to the antioxidant activities of cou marins.
Abstract: The aim of this work was to study the structure-activity relationships of the antioxidant activity of natural coumarins isolated from four Korean medicinal plants (1-17) and four purchased coumarins (18-21). The free radical scavenging and lipid peroxidation assays revealed that five phenolic coumarins, scopoletin (1), aesculetin (2), fraxetin (3), umbelliferone (18) and daphnetin (19), possessed considerable antioxidant activities. The coumarins having a catechol group, 2, 3 and 19, showed significant free radical scavenging activity and inhibitory effects on lipid peroxidation, indicating that the catechol group significantly contributed to the antioxidant activities of coumarins. In contrast, the sugar moiety markedly reduced the activities of coumarin glycosides. The results also demonstrate that the alpha-pyrone ring of coumarins significantly enhanced the capacity of inhibiting oxidative reactions of coumarins.
118 citations
TL;DR: Three new homoisoflavonoids (1-3) were isolated from the roots of Ophiopogon japonicus (Liliaceae) and their effects on the release of the inflammatory chemokine eotaxin, stimulated by IL-4 and the combination of TNF-alpha in BEAS-2B cells, which mimics the in vivo conditions in bronchial allergic asthma.
58 citations
TL;DR: Four new lanostane triterpenes were isolated from the fruiting bodies of Ganoderma lucidum together with 14 known compounds and the inhibitory effect of isolated compounds on adipocyte differentiation in 3T3-L1 cells was examined.
Abstract: Four new lanostane triterpenes, butyl ganoderate A (1), butyl ganoderate B (2), butyl lucidenate N (3), and butyl lucidenate A (4), were isolated from the fruiting bodies of Ganoderma lucidum together with 14 known compounds (5-18). The structures of the new triterpenes were established by extensive spectroscopic studies and chemical evidence. In addition, the inhibitory effect of isolated compounds on adipocyte differentiation in 3T3-L1 cells was examined.
48 citations
TL;DR: PG as an arginase inhibitor represents a novel molecule for the therapy of cardiovascular diseases derived from endothelial dysfunction and may be used for the design of pharmaceutical compounds.
Abstract: Arginase competitively inhibits nitric oxide synthase (NOS) via use of the common substrate L-arginine. Arginase II has recently reported as a novel therapeutic target for the treatment of cardiovascular diseases such as atherosclerosis. Here, we demonstrate that piceatannol-3'-O-β-D-glucopyranoside (PG), a potent component of stilbenes, inhibits the activity of arginase I and II prepared from mouse liver and kidney lysates, respectively, in a dose-dependent manner. In human umbilical vein endothelial cells, incubation of PG markedly blocked arginase activity and increased NOx production, as measured by Griess assay. The PG effect was associated with increase of eNOS dimer ratio, although the protein levels of arginase II or eNOS were not changed. Furthermore, isolated mice aortic rings treated with PG showed inhibited arginase activity that resulted in increased nitric oxide (NO) production upto 78%, as measured using 4-amino-5-methylamino-2',7'-difluorescein (DAF-FM) and a decreased superoxide anions up to 63%, as measured using dihydroethidine (DHE) in the intact endothelium. PG showed IC50 value of 11.22 µM and 11.06 µM against arginase I and II, respectively. PG as an arginase inhibitor, therefore, represents a novel molecule for the therapy of cardiovascular diseases derived from endothelial dysfunction and may be used for the design of pharmaceutical compounds.
47 citations
TL;DR: N. nucifera stamens-derived compounds could potentially exert their primary anti-Alzheimer effects as AChE inhibitors rather than BACE1 inhibitors.
Abstract: A new beta-cyclogeraniol diglycoside (5), along with four known components, cycloartenol (1), p-hydroxybenzoic acid (2), vanilloloside (3), and 5'-O-methyladenosine (4), were first isolated from the n-BuOH fraction of Nelumbo nucifera stamens. The chemical structure of 5 was elucidated as 1-hydroxymethyl-2,6,6-trimethyl-1-cyclohexene 9-O-beta-D-glucopyranosyl-(1-->6)-beta-D-glucopyranoside (nuciferoside) on the basis of chemical and spectroscopic evidence, including 1D, 2D NMR, and MS. The anti-Alzheimer effects of 1-5 were evaluated via the acetylcholinesterase (AChE), butyrylcholinesterase (BChE), and beta-site amyloid precursor protein (APP) cleaving enzyme 1 (BACE1) inhibition assays. Compounds 1-3 and 5 showed good and noncompetitive inhibition against AChE with IC(50) values of 11.89, 20.07, 4.55, and 3.20 microM and K(i)values of 15.71, 25.44, 7.76, and 5.76 microM, respectively. Compounds 1, 2, and 5 also possessed BChE inhibitory activities with IC(50) values of 13.93, 62.29, 205.78, and 83.06 microM, respectively. The selectivity index (SI) values of 1, 2, 3, and 5, calculated from IC(50) values of BChE and AChE, were 1.2, 3.1, 45.7, and 26.0. However, all isolated compounds lacked BACE1 inhibition up to 100 microM. Therefore, N. nucifera stamens-derived compounds could potentially exert their primary anti-Alzheimer effects as AChE inhibitors rather than BACE1 inhibitors.
46 citations
TL;DR: Activity-directed isolation of the ethyl acetate, methylene chloride and n-hexane fractions of Gloiopeltis furcata resulted in the isolation of 18 compounds, whose structures were elucidated as 2-(3-hydroxy-5-oxotetrahydrofuran-3-yl)acetic acid, which most of the compounds isolated were lacking the scavenging activity on DPPH radical and ONOO(-).
Abstract: Activity-directed isolation of the ethyl acetate, methylene chloride and n-hexane fractions of Gloiopeltis furcata resulted in the isolation of 18 compounds. Their structures were elucidated as 2-(3-hydroxy-5-oxotetrahydrofuran-3-yl)acetic acid (1), glutaric acid (2), succinic acid (3), nicotinic acid (4), (E)-4-hydroxyhex-2-enoic acid (5), cholesterol (6), 7-hydroxycholesterol (7), uridine (8), glycerol (9), 5-(hydroxymethyl)-2-methoxybenzene-1,3-diol (10), (5E,7E)-9-oxodeca-5,7-dienoic acid (11), (Z)-3-ethylidene-4-methylpyrrolidine-2,5-dione (12), dehydrovomifoliol (13), loliolide (14), cholesteryl stearate (15), palmitic acid (16), cis-5,8,11,14,17-eicosapentaenoic acid (17) and alpha-linolenic acid (18) on the basis of spectroscopic and chemical evidences. Their anticholinesterase and antioxidant activities were evaluated via inhibitory activities on acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) as well as scavenging activities on 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical and peroxynitrite (ONOO(-)). All isolated compounds (1-18) exhibited moderate AChE inhibitory activities with IC(50) values ranging from 1.14-12.50 microg/ml, whereas 1, 7, 9, 17, and 18 showed mild BChE inhibitory activities with IC(50) values ranging from 5.57-15.89 microg/ml. Although most of the compounds isolated were lacking the scavenging activity on DPPH radical and ONOO(-), 5 and 10 showed good DPPH radical scavenging activity, and 5, 10, and 16 showed potent ONOO(-) scavenging activity.
43 citations
TL;DR: Findings demonstrate a potential to develop these flavonoids and isoflavonoids as hypoglycemic drugs, and Stimulating glucose uptake into peripheral cells may be responsible for reducing the level of blood glucose in the circulation.
Abstract: Glucose uptake assay-guided fractionations on the methanol extract of Sophorae Flos led to the isolation of the flavonoids rutin (1), narcissin (2), quercetin (3), tamarixetin (4), and kaempferol (5) and the isoflavonoids cajanin (6), genistein (7), orobol (8), and pratensein (9). Among them, 1, 4, 5, 6, 8, and 9 significantly improved basal glucose uptake in HepG2 cells. Their improving effects were concentration dependent. Compounds 4, 5, 6, and 9 exhibited effects stronger than that of rosiglitazone, which has been used as an antidiabetic drug. However, 2, 3, and 7 did not show any improving effects. Stimulating glucose uptake into peripheral cells may be responsible for reducing the level of blood glucose in the circulation. Therefore, these findings demonstrate a potential to develop these flavonoids and isoflavonoids as hypoglycemic drugs.
43 citations
TL;DR: The structure of the new triterpene was determined to be 5alpha,6alpha-epidioxy-5beta,6beta-epoxy-9,13-dimethyl-25,26-dinoroleanan-3beta-ol acetate by spectroscopic studies.
43 citations
TL;DR: Five flavonoids, myricetin-3′,5′-dimethylether 3-O-β-d-galactopyranoside, quercetin, kaempferol, and tamarixetin were isolated from the buds of Cleistocalyx operculatus (Myrtaceae) and showed potential inhibitory activities against acetylcholinesterase and BChE.
Abstract: Five flavonoids, myricetin-3′-methylether 3-O-β-d-galactopyranoside (1), myricetin-3′,5′-dimethylether 3-O-β-d-galactopyranoside (2), quercetin (3), kaempferol (4), and tamarixetin (5) were isolated from the buds of Cleistocalyx operculatus (Myrtaceae). The chemical structures of these compounds were determined on the basis of spectroscopic analyses, including 2D NMR. Their anti-Alzheimer effects were evaluated via acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) inhibitory activity assays. All five compounds 1–5 showed potential inhibitory activities against AChE with IC50 values of 19.9, 37.8, 25.9, 30.4 and 22.3 μM, respectively, while compounds 1, 3, 4 and 5 also possessed BChE inhibitory activity with IC50 values of 152.5, 177.8, 62.5, and 160.6 μM, respectively.
41 citations
TL;DR: From the ethyl acetate fraction of the stem bark of Camellia japonica, three new triterpenoids are derived, which showed cytotoxicity against LLC and HL-60 cancer cell lines with IC(50) values of 25.2 and 21.7 microM, respectively.
Abstract: From the ethyl acetate fraction of the stem bark of Camellia japonica, three new triterpenoids, 3beta-O-acetyl-16beta-hydroxy-12-oxoolean (6), 3beta-O-acetyl-16beta-hydroxy-11-oxoolean-12-ene (7), and 3beta-O-acetyl-16beta-hydroxyolean-12-ene (8), along with seven known compounds, 3alpha-hydroxy-1-oxofriedelan (1), friedelin (2), 3beta-friedelanol (3), canophyllol (4), 3-oxofriedelan-1(2)-ene (5), beta-amyrin (9), camellenodiol (10), and camelledionol (11) were isolated. Their structures were established on the basis of spectroscopic analysis and chemical evidence. The isolated compounds were tested in vitro for their cytotoxic activities against the A549, LLC, HL-60 and MCF-7 cancer cell lines. Among them, compound 8 showed cytotoxicity against LLC and HL-60 cancer cell lines with IC(50) values of 25.2 and 21.7 microM, respectively.
40 citations
TL;DR: It is demonstrated that the lanostane triterpenes isolated in this study contribute to the inhibitory effect of the fruiting bodies of G. LUCIDUM on adipocyte differentiation in 3T3-L1 cells.
Abstract: Two new lanostane triterpenes, methyl lucidenate N ( 1) and T-butyl lucidenate B ( 2), were isolated from the fruiting bodies of GANODERMA LUCIDUM together with five known compounds ( 3- 7). The structures of the two new triterpenes were established as methyl 3 β,7 β-dihydroxy-4,4,14 α-trimethyl-11,15-dioxo-5 α-chol-8-en-24-oate ( 1) and T-butyl 7 β,12 β-dihydroxy-4,4,14 α-trimethyl-3,11,15-trioxo-5 α-chol-8-en-24-oate ( 2) by extensive spectroscopic studies and chemical evidence. The effect of the isolated compounds ( 1- 7) on triglyceride (TG) accumulation, an indicator of adipocyte differentiation, during the differentiation of 3T3-L1 preadipocytes was examined. T-Butyl lucidenate B ( 2) reduced the TG accumulation significantly by 72 % at 80 μM compared to the untreated group. Furthermore, compound 2 effectively suppressed the GPDH activity in the cells. Consistent with the decrease in TG accumulation and GPDH activity, compound 2 suppressed the gene expressions of PPAR γ, C/EBP α, and SREBP-1c in a dose-dependent manner during differentiation. Our findings demonstrate that the lanostane triterpenes isolated in this study contribute to the inhibitory effect of the fruiting bodies of G. LUCIDUM on adipocyte differentiation in 3T3-L1 cells.
TL;DR: Two novel neo-clerodane diterpenoids, barbatellarines A and B, were isolated from the whole plants of Scutellaria barbata and evaluated for in vitro cytotoxic activity against the HL-60, MCF7, and LLC cancer cell lines.
TL;DR: Results demonstrate that inhibition of adipogenesis in 3T3-L1 cells by treatment with 1 and 3 may be mediated in part through down-regulation of the adipogenic transcription factor SREBP-1c and its target genes, such as FAS and ACC.
TL;DR: The findings suggest that GFE and geniposide may ameliorate the treatment of ligament injuries by proliferating ligament fibroblasts and promoting the synthesis of collagen, and the use of diclofenac to treat acute ligaments injuries should be reassessed although it possesses a potential effect on relieving symptoms.
Abstract: Gardeniae Fructus is a traditional medicine used for the treatment of contusion such as ankle sprain Geniposide is one of the main components of Gardeniae Fructus with diverse biological activities In order to gain further insight into the therapeutic action of Gardeniae Fructus extract (GFE) and geniposide on ligament injuries, a new in vitro model was developed in the present study Rat hind ankle ligament fibroblasts (RHALFs) derived from Sprague-Dawley rats were cultured, and the cell proliferation and collagen content were examined by MTT and a Sirius Red-based colorimetric assay after stimulating with each drug The cell growth of RHALFs was promoted by culturing with 375-150 microg/mL of GFE and 25-200 microM of geniposide The content of collagen in the RHALFs was significantly increased up to 1314% and 1242% of the control value by culturing with the GFE and geniposide, respectively By contrast, both cell growth and collagen content were impaired by adding 25-200 microM of diclofenac, one of the common medications for ligament injuries The findings suggest that GFE and geniposide may ameliorate the treatment of ligament injuries by proliferating ligament fibroblasts and promoting the synthesis of collagen However, the use of diclofenac to treat acute ligament injuries should be reassessed although it possesses a potential effect on relieving symptoms
TL;DR: In this paper, two new norlignans, cedralins A (1) and B (2), were isolated from the leaves of Cedrela sinensis and their structures were established by extensive spectroscopic studies and chemical evidence.
Abstract: Two new norlignans, cedralins A (1) and B (2), were isolated from the leaves of Cedrela sinensis. Their structures were established by extensive spectroscopic studies and chemical evidence. The absolute configurations of these compounds were determined by comparing their CD spectra with those of known compounds. In addition, their in vitro cytotoxic activity against two human-cancer cell lines was evaluated.
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TL;DR: In this article, the chemical structures of the roots of Aralia cordata (syn.=A. continentalis) were elucidated through spectroscopic method, and 14 compounds were isolated from the n-BuOH fraction of the root.
Abstract: Fourteen compounds were isolated from the n-BuOH fraction of the roots of Aralia cordata (syn.=A. continentalis). Through spectroscopic method, the chemical structures were elucidated as: caffeic acid (1), protocatechuic acid (2), thymidine (3), uridine (4), methyl-α-D-fructofuranoside (5), a mixture (3:1) of β-D-fructopyranoside and β-D-fructofuranoside (6), 1-methyl 1,2,3,4-tetrahydro-β-carboline-3-carboxylic acid (7), methyl-β-D-fructofuranoside (8), sucrose (9), 5-caffeoylquinic acid (chlorogenic acid) (10), 3-caffeoylquinic acid (neochlorogenic acid) (11), 4-caffeoylquinic acid (cryptochlorogenic acid) (12), 3,5-di-O-caffeoylquinic acid (13), and 1-kestose [β-D-fructofuranosyl-(2→ 1)-β-D-fructofuranosyl-(2→ 1)-α-D-glucopyranoside] (14). Among them, compounds 5, 7, 8, and 10-14 were isolated from this plant for the first time.
TL;DR: Four new 28-nor-oleanane-type triterpene oligoglycosides, isolated from the stem bark of Camellia japonica, showed inhibitory effects on NO production in RAW264.7 macrophages.
TL;DR: In the course of screening plants used in folk medicine as memory enhancers, a 70% ethanolic extract of Stephania rotunda roots showed significant AChE inhibitory activity, which led to the isolation of a new protoberberine alkaloid, which is named stepharotudine.
Abstract: In the course of screening plants used in folk medicine as memory enhancers, a 70% ethanolic extract of Stephania rotunda roots showed significant AChE inhibitory activity. Repeated column chromatography led to the isolation of a new protoberberine alkaloid, which we named stepharotudine (1), and seven known compounds (2-8). The chemical structures of the isolated compounds were elucidated based on extensive 1D and 2D NMR spectroscopic data. Compounds 1-8 were investigated in vitro for their anticholinesterase activity using a rat cortex AChE enzyme.
TL;DR: In this article, 50 µL of the MTT solution (1.1 mg/mL) was added to each well and incubated for an additional 4 h. The colored formazan crystal was dissolv-ed in 150 µl of dimethyl sulfoxide (DMSO).
Abstract: per well) were added to a serial dilution of compounds in 96-well plates. After incubation for 44 h, 50 µL of the MTT solution (1.1 mg/mL) was added to each well and incubated for an additional 4 h. The colored formazan crystal produced from the MTT was dissolv-ed in 150 µL of dimethyl sulfoxide (DMSO). The optical density (OD) values of the solutions were measured at 540 nm using a plate reader.
TL;DR: A n‐hexane soluble fraction prepared from the fruit of Schisandra chinensis (Schisandraceae) was found to inhibit FAS activity at 100 μg/mL, suggesting a further study on the anticancer potential of dibenzocyclooctadiene derivatives.
Abstract: Inhibition of fatty acid synthase (FAS) has been proposed to be a new therapeutic target for the treatment of cancer and obesity. In our preliminary screening study on the FAS inhibitory activity, a n-hexane soluble fraction prepared from the fruit of Schisandra chinensis (Schisandraceae) was found to inhibit FAS activity at 100 microg/mL. Nine dibenzocyclooctadiene lignans were isolated from the active fraction and were evaluated for their inhibitory effect on FAS for the first time. The compounds possessing a benzoyl or tigloyl group in the dibenzocyclooctadiene skeleton entirely inhibited the FAS activity in a dose dependent manner. The findings may be partially related to the anticancer effect of the medicinal plant, suggesting a further study on the anticancer potential of dibenzocyclooctadiene derivatives.
TL;DR: Commercial available rhus product is comprised of material considered to be urushiol dimer, and even if the antigenicity of the rhus products is low, this product may cause adverse effects and is not completely detoxified.
Abstract: Background: Systemic contact dermatitis commonly occurs with the intake of rhus (boiled chicken with rhus) as a health food and a folk medicine to cure gastrointestinal diseases in Korea. Rhus companies insist they have the technology for rhus detoxification. However, the numbers of systemic allergic contact dermatitis patients, caused by rhus, have not decreased. The principle of present techniques for rhus detoxification is the induction of the polymerization of urushiol, but polymerized urushiol may still have antigenicity, although to a diminished degree. The Korean Food and Drug Administration (KFDA) has a regulation to control urushiol use as a food. However, the laboratory method that KFDA uses for detection of rhus can only detect the urushiol monomer. Objective: We conducted experiments to detect polymerized urushiol in rhus products, which were considered not to include urushiol by the KFDA. Methods: Rhus product approved by the KFDA was separated with chloroform. The chloroform fractionation was accomplished on a recycle HPLC system. Four peaks were achieved and evaporated to give an amorphous powder. Each powder was analyzed on a NMR system and mass spectrometer. Results: The material considered to be urushiol dimer, with a 638 molecular weight (MW), was detected in one of the four powders as per the HPLC peaks. Conclusion: We concluded that commercially available rhus product is comprised of material considered to be urushiol dimer. Therefore, even if the antigenicity of the rhus products is low, this product may cause adverse effects and is not completely detoxified. (Ann
TL;DR: The results obtained in this study demonstrate that overall gene expression profiles of the extracts correlated well with their biological activity and that CEXs prepared from plants of diverse origins vary in their biological activities.
Abstract: In this study, we attempted to determine the anti-inflammatory activity of a medicinal plant huang-lian using gene expression profiles as an index. Huang-line extracts (CEXs) were prepared from seven different plant origins and compared for their chemical composition and biological activity. In order to achieve this, RAW264.7 cells were treated with CEXs in the absence or presence of LPS for 6 h, and the differential gene expression profiles were analyzed using oligonucleotide microarrays. The alkaloid content of CEXs was determined by high performance liquid chromatography analysis. Evaluation of anti-inflammatory activity of CEXs was by measuring a decrease in cytokines and nitric oxide production in LPS-stimulated RAW264.7 cells. Hierarchical clustering analysis revealed that three CEXs from Coptis chinensis formed a cluster separate from the other four CEXs in LPS-stimulated cells, and were the most effective anti-inflammatoryagents. The extract prepared from Picrorrhiza kurrooa neither induced any changes in gene expression profiles nor possessed any anti-inflammatory activity. The extract from Jeffersonia dubia, which exhibited the highest cytotoxicity among the CEXs tested, was most effective in suppressing LPS-induced nitric oxide production but was not able to inhibit proinflammatory cytokine production. The results obtained in this study demonstrate that overall gene expression profiles of the extracts correlated well with their biological activity and that CEXs prepared from plants of diverse origins vary in their biological activity. These data also suggest that gene expression profiles may serve as a good indicator for the pharmacological activities of medicinal plants arising from diverse origins.
TL;DR: Results suggested that the essential oil of the C. operculatus buds might exert an anti-inflammatory effect by suppressing the expression of pro-inflammatory cytokines, which is mediated, at least in part, by blocking the NF-κB activation.
Abstract: Cleistocalyx operculatus (Roxb.) Merr and Perry (Myrtaceae) is a well-known medicinal plant, widely distributed and propagated in China, Vietnam and some other tropical countries. Several species of Cleistocalyx are used in folk medicine. In Vietnam, C. operculatus is commonly called “Voi”. The flower buds (“Nu Voi”) and leaves (“La Voi”) have been used to make a beverage since ancient times. 1 The buds are commonly used as an ingredient for tonic drinks in Southern China. 2 The water extract of C. operculatus buds was shown to increase the contractility and decrease the frequency of contraction in an isolated rat heart perfusion system. 3 It showed strong protective effects on lipid peroxidation in rat liver microsomes and on the H2O2induced trauma of PC12 cells. 4 C. operculatus extract showed inhibitory activity against the α-glucosidase, rat-intestinal maltase, sucrase activities, and is considered a promising material for preventing and treating diabetes. 5 Recently, the essential oil of the C. operculatus buds was investigated for its in vitro and in vivo anti-inflammatory activities. These results suggested that its essential oil might exert an anti-inflammatory effect by suppressing the expression of pro-inflammatory cytokines, which is mediated, at least in part, by blocking the NF-κB activation. 6
TL;DR: In this article, two guaiane sesquiterpenoids, 11-(acetyloxy)-1,8-dihydroxyguai-4-en-3-one (5) and (1α,6β)1,6-Dihydroxymethyltorilin (6), were isolated from the fruits of Torilis japonica (Umbelliferae), along with four known sesquerpenes, torilin(1), torilolone (2), (1β)-1-hydroxy
Abstract: Two new guaiane sesquiterpenoids, 11-(acetyloxy)-1,8-dihydroxyguai-4-en-3-one (5) and (1α,6β)-1,6-dihydroxytorilin (6), were isolated from the fruits of Torilis japonica (Umbelliferae), along with four known sesquiterpenes, torilin (1), torilolone (2), (1β)-1-hydroxytorilin (3), and (1α)-1-hydroxytorilin (4). During the phytochemical investigation, daucosterol, friedelin, and epifriedelanol were also isolated from the plant for the first time. The structures of the new sesquiterpenoids 5 and 6 were determined by comprehensive analyses of MS and NMR spectroscopic data. These isolates were evaluated against human breast cancer cells (MCF-7) and Lewis lung carcinoma (LLC) cells. Compounds 1, 3, and 4 exhibited cytotoxic activity against the LLC cells with IC50 values of 31.3, 32.5, and 34.0 μg/ml, respectively. However, no significant cytotoxicity was found against the MCF-7 cells for any of the compounds tested.
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TL;DR: In this paper, a new monoterpene glycoside (3) was determined by spectroscopic data interpretation and physico-chemical properties, which exhibited a remarkable inhibitory activity against lipoxygenase-1 with IC50 values of 45.2 and 37.5 ± 0.7?M, respectively.
Abstract: Phytochemical investigation on the ethyl acetate and n-butanol fractions of Moutan Cortex resulted in the isolation and characterization of a new monoterpene glycoside (3) and twenty known monoterpene glycosides (1, 2, 4 -21). The structure of 3 was determined by spectroscopic data interpretation and physico-chemical properties. Compounds 1 and 8 presented a remarkable inhibitory activity against lipoxygenase-1 (LOX-1) with IC50 values of 45.2 and 37.5 ?M, respectively. Compounds 9, 10, 13, 18, 19, and 21 showed significant 1, 1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging effect with IC50 values of 9.8, 25.5, 6.4, 15.2, 18.7, and 23.7 ?M, respectively. Benzoylpaeoniflorin (8), which exhibited the highest inhibitory effect with an IC50 value of 37.5 ± 0.7 ?M, was further analyzed the inhibition kinetics by Lineweaver-Burk plots. Results indicated that 8 is a non-competitive inhibitor, and the kinetic parameter values were estimated to be (31.04 ?M, Ki), (0.29 ?M/min, Vm), and (48.50 ?M, Km).
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Journal Article•
TL;DR: A simple reversed phase HPLC method was developed for extracting pharmacologically active compounds scopolin, chlorogenic acid, 2,4-dihydroxy-6-methoxyacetophenone 4-glycoside, hyperoside, isorhamnetin 3-O-robinobioside, and scoparone from Artemisia capillaris using a binary gradient of acetonitrile: 0.1% trifluoroacetic acid with UV detection at 254 nm.
Abstract: In order to facilitate the quality control of Artemisia capillaris, a simple, accurate and reliable HPLC method was developed for the simultaneous determination of the six bioactive compounds: scopolin (1), chlorogenic acid (2), 2,4-dihydroxy-6-methoxyacetophenone 4-glycoside (3), hyperoside (4), isorhamnetin 3-Orobinobioside (5), and scoparone (6), which were selected as the chemical markers of A. capillaris. Separation was achieved on an Agilent Eclipse XDB-C18 column with a gradient solvent system of 0.1% trifluoroacetic acid aqueous-acetonitrile at a flow-rate of 1.0 mL/min and detected at 254 nm. All six calibration curves showed good linearity (R2>0.998). A simple reversed phase HPLC method was developed for extracting pharmacologically active compounds scopolin, chlorogenic acid, 2,4-dihydroxy-6-methoxyacetophenone 4-glycoside, hyperoside, isorhamnetin 3-O-robinobioside, and scoparone from A. capillaris using a binary gradient of acetonitrile: 0.1% trifluoroacetic acid with UV detection at 254 nm. The scopolin (1), chlorogenic acid (2), 2,4-dihydroxy-6-methoxyacetophenone 4-glycoside (3), hyperoside (4), isorhamnetin 3-O-robinobioside (5), and scoparone (6) contents of the herb of A. capillaris collected from fifteen district markets in Korea were 0.00~0.90 mg/g, 0.06~7.29 mg/g, 0.06~0.91 mg/g, 0.07~5.05 mg/g, 0.42~13.11 mg/g, and 1.11~29.82 mg/g, respectively. The results demonstrated that this method is simple and reliable for the quality control of A. capillaris.
TL;DR: In this article, the isolation, structure elucidation and anti-inflammatory activities of ophiopogonanone G (I), Ophiopogenone A (IIa), and Ophogenogenone B (IIb) were investigated.
Abstract: isolation, structure elucidation and antiinflammatory activities of ophiopogonanone G (I), ophiopogoside A (IIa), and ophiopogoside B (IIb)
TL;DR: A new stereoisomer of a tetrahydrofuranoid lignan, acerifuranoid A (1), and two oleanane-type triterpenoids (J and K) were isolated from the roots of Aceriphyllum rossii and their structures were elucidated on the basis of spectroscopic analyses and chemical evidence as discussed by the authors.
Abstract: A new stereoisomer of a tetrahydrofuranoid lignan, acerifuranoid A (1), and two new oleanane-type triterpenoids, aceriphyllic acids J and K (2 and 3), were isolated from the roots of Aceriphyllum rossii. Their structures were elucidated on the basis of spectroscopic analyses and chemical evidence. These isolated compounds exhibited weak cytotoxic activity against various cancer cell lines with IC50>150 μM.