Chi Keung Cheng

TL;DR: The mushroom Coprinopsis cinerea is a classic experimental model for multicellular development in fungi because it grows on defined media, completes its life cycle in 2 weeks, produces some 108 synchronized meiocytes, and can be manipulated at all stages in development by mutation and transformation.

TL;DR: This study coupled 5'-Serial Analysis of Gene Expression (5'-SAGE) to high-throughput pyrosequencing from 454 Life Sciences to analyze the transcriptomes and identify up-regulated genes among vegetative mycelium and stage 1 primordium of Coprinopsis cinerea during fruiting body development, implicated a wealth of new candidate genes important to early stages of mushroom fruiting development.

TL;DR: These findings provided the first evidence for milRNAs in the model mushroom and their potential roles in regulating fruiting body development and new information on the evolutionary relationship of milRNA biogenesis proteins across kingdoms has provided new insights for guiding further functional and evolutionary studies of miRNAs.

TL;DR: These findings provided the first evidence of milRNAs in the model mushroom and their potential roles in regulating fruiting body development and provided new insights into further functional and evolutionary studies of miRNAs.

TL;DR: The utility of next-generation sequencing coupled to traditional microbiological testing method in a retrospective epidemiological study involving multiple clinical isolates and two distinct clades of S. Typhimurium isolates is demonstrated.