Claire Burns

TL;DR: The mushroom Coprinopsis cinerea is a classic experimental model for multicellular development in fungi because it grows on defined media, completes its life cycle in 2 weeks, produces some 108 synchronized meiocytes, and can be manipulated at all stages in development by mutation and transformation.

TL;DR: A "Molecular Toolkit" comprising interchangeable promoters and marker genes to facilitate transformation of homobasidiomycete mushrooms is developed and it is demonstrated that a prerequisite for GFP expression in C. cinereus and A. bisporus is the presence of an intron.

TL;DR: Coprinopsis cinerea laccase gene lcc1 was expressed in this basidiomycete under naturally non-inductive conditions using various homologous and heterologous promoters and addition of copper to the medium increased enzymatic activities for highly active transformant by 10- to 50-fold and for less active transformants for 2- to 7-fold.

TL;DR: Verticillium fungicola, a severe mycopathogen of the cultivated mushroom Agaricus bisporus, was successfully transformed using both PEG-mediated and Agrobacterium-mediated techniques and showed integration of the transforming genes into the target genome.

TL;DR: It is found that meiotic genes are significantly more conserved in their transcript profiles than genes not known to be meiotic, which indicates a remarkable conservation of the meiotic process across evolutionarily distant organisms.