Claudia Giesecke

TL;DR: The fidelity of VH:VL pairs identified by this approach were validated and the method was used to sequence the repertoire of three human cell subsets—peripheral blood IgG+ B cells, peripheral plasmablasts isolated after tetanus toxoid immunization and memory B cells isolated after seasonal influenza vaccination.

TL;DR: New information has made it possible to distinguish between the contribution played by abnormalities in central checkpoints that could lead to a pre-immune repertoire enriched in autoreactive B cells, and the possibility that autoimmunity arises in the periphery from somatic hypermutation and abnormal selection during T cell-dependent B-cell responses on the other.

TL;DR: The results suggest that only a small fraction of responding peripheral B cells give rise to the bone marrow long-lived plasma cells responsible for the production of biologically relevant amounts of vaccine-specific antibodies in the serological memory response 9 mo postvaccination.

TL;DR: A multilayer model of PC memory is proposed in which CD19(+) andCD19(-) PC represent dynamic and static components, respectively, permitting both adaptation and stability of humoral immune protection.

TL;DR: The specificity and efficiency of XCR1-mediated Ag targeting to cross-presenting DC, combined with its lack of adverse effects, make this system a prime candidate for the development of therapeutic cytotoxic vaccines in humans.