Clay Bracken

TL;DR: A rapid and efficient approach for preparing isotopically labeled recombinant proteins is presented and expression yields obtained provide a fourfold to eightfold reduction in isotope costs using simple shake flask growths.

TL;DR: SS‐31 (D‐Arg‐dimethylTyr‐Lys‐Phe‐NH2) selectively binds to cardiolipin and inhibits cytochrome c peroxidase activity, and it is examined whether SS‐31 also protected the electron carrier function of cy tochrome c.

TL;DR: Results suggest that, for the basic region, lowering the temperature increases the population of transient helical conformations, and concomitantly reduces the amplitude or timescale of conformational fluctuations on picosecond-nanosecond timescales.

TL;DR: It is shown that the isolated BDNF prodomain is detected in the hippocampus and that it can be secreted from neurons in an activity-dependent manner and identifies the Met66 prodomain as a new active ligand which modulates neuronal morphology.

TL;DR: This analysis provides a simple method for rapidly estimating and dissociating the effects of motional anisotropy and chemical exchange in NMR heteronuclear spin relaxation data and demonstrates the utility of the method with 15N relaxation data collected on the proteins E. coli ribonuclease H and the trimeric E coli membrane associated lipoprotein lpp.