D
Daniel Broek
Researcher at University of Southern California
Publications - 47
Citations - 6983
Daniel Broek is an academic researcher from University of Southern California. The author has contributed to research in topics: Saccharomyces cerevisiae & Guanine nucleotide exchange factor. The author has an hindex of 28, co-authored 47 publications receiving 6867 citations. Previous affiliations of Daniel Broek include Cold Spring Harbor Laboratory.
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Journal ArticleDOI
Purification of a RAS-responsive adenylyl cyclase complex from Saccharomyces cerevisiae by use of an epitope addition method
Jeffrey Field,J. Nikawa,Daniel Broek,B. MacDonald,Linda Rodgers,Ian A. Wilson,Richard A. Lerner,Michael Wigler +7 more
TL;DR: A method for immunoaffinity purification of Saccharomyces cerevisiae adenylyl cyclase based on creating a fusion with a small peptide epitope based on oligonucleotide technology is developed.
Journal ArticleDOI
In yeast, RAS proteins are controlling elements of adenylate cyclase
T. Toda,Isao Uno,Tatsuo Ishikawa,Scott Powers,Tohru Kataoka,Daniel Broek,Scott J. Cameron,James R. Broach,Kunihiro Matsumoto,Michael Wigler +9 more
TL;DR: S. cerevisiae strains containing RAS2val19, a Ras2 gene with a missense mutation analogous to one that activates the transforming potential of mammalian ras genes, have growth and biochemical properties strikingly similar to yeast strains carrying IAC or bcy1.
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Role of Substrates and Products of PI 3-kinase in Regulating Activation of Rac-Related Guanosine Triphosphatases by Vav
Jaewon Han,Katherine Luby-Phelps,Balaka Das,Xiaodong Shu,Yi Xia,Raymond D. Mosteller,U. Murali Krishna,John R. Falck,Michael A. White,Daniel Broek +9 more
TL;DR: Control of Vav in response to mitogens by the products of PI 3-kinase suggests a mechanism for Ras-dependent activation of Rac.
Journal ArticleDOI
The S. cerevisiae CDC25 gene product regulates the RAS/adenylate cyclase pathway
Daniel Broek,T. Toda,T. Michaeli,Lonny R. Levin,C. Birchmeier,Mark J. Zoller,Scott Powers,Michael Wigler +7 more
TL;DR: It is proposed that CDC25 regulates adenylate cyclase by regulating the guanine nucleotide bound to RAS proteins, suggesting that normal RAS is dependent on CDC25 function.
Journal ArticleDOI
DNA sequence and characterization of the S. cerevisiae gene encoding adenylate cyclase
TL;DR: Gene disruption and biochemical experiments indicate that the catalytic domain of adenylate cyclase resides in the carboxyl terminal 400 amino acids, which indicates that elevated expression ofadenylates cyclase suppresses the lethality that otherwise results from loss of RAS gene function in yeast.