D
Darinka D. Klumpers
Researcher at Harvard University
Publications - 9
Citations - 2695
Darinka D. Klumpers is an academic researcher from Harvard University. The author has contributed to research in topics: Cellular differentiation & Self-healing hydrogels. The author has an hindex of 8, co-authored 9 publications receiving 2046 citations. Previous affiliations of Darinka D. Klumpers include VU University Medical Center & Wyss Institute for Biologically Inspired Engineering.
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Journal ArticleDOI
Hydrogels with tunable stress relaxation regulate stem cell fate and activity
Ovijit Chaudhuri,Ovijit Chaudhuri,Ovijit Chaudhuri,Luo Gu,Luo Gu,Darinka D. Klumpers,Darinka D. Klumpers,Darinka D. Klumpers,Max Darnell,Max Darnell,Sidi A. Bencherif,Sidi A. Bencherif,James C. Weaver,Nathaniel Huebsch,Hong-pyo Lee,Evi Lippens,Evi Lippens,Georg N. Duda,David J. Mooney,David J. Mooney +19 more
TL;DR: It is found that cell spreading, proliferation, and osteogenic differentiation of mesenchymal stem cells (MSCs) are all enhanced in cells cultured in gels with faster relaxation, highlighting stress relaxation as a key characteristic of cell-ECM interactions and as an important design parameter of biomaterials for cell culture.
Journal ArticleDOI
Substrate stress relaxation regulates cell spreading
Ovijit Chaudhuri,Luo Gu,Max Darnell,Darinka D. Klumpers,Sidi A. Bencherif,James C. Weaver,Nathaniel Huebsch,David J. Mooney +7 more
TL;DR: Surprisingly, both the computational model and experiments find that spreading for cells cultured on soft substrates that exhibit stress relaxation is greater than cells spreading on elastic substrates of the same modulus, but similar to that of cells spread on stiffer elastic substrate.
Journal ArticleDOI
Substrate stiffening promotes endothelial monolayer disruption through enhanced physical forces
Ramaswamy Krishnan,Darinka D. Klumpers,Chan Young Park,Kavitha Rajendran,Xavier Trepat,Jan van Bezu,Victor W.M. van Hinsbergh,Christopher V. Carman,Joseph D. Brain,Jeffrey J. Fredberg,James P. Butler,Geerten P. van Nieuw Amerongen +11 more
TL;DR: A central role of physical forces in EC gap formation is demonstrated and a novel physiological mechanism is highlighted, which in normal circumstances is compliant but during pathology becomes stiffer.
Journal ArticleDOI
Influence of the stiffness of three-dimensional alginate/collagen-I interpenetrating networks on fibroblast biology.
Cristiana Branco da Cunha,Darinka D. Klumpers,Darinka D. Klumpers,Darinka D. Klumpers,Weiwei Aileen Li,Weiwei Aileen Li,Sandeep T. Koshy,Sandeep T. Koshy,Sandeep T. Koshy,James C. Weaver,Ovijit Chaudhuri,Pedro L. Granja,Pedro L. Granja,David J. Mooney,David J. Mooney +14 more
TL;DR: Findings suggest that simply modulating the matrix mechanical properties of a given wound dressing biomaterial deposited at the wound site could regulate the progression of wound healing.
Journal ArticleDOI
CD44 alternative splicing in gastric cancer cells is regulated by culture dimensionality and matrix stiffness.
Cristiana Branco da Cunha,Darinka D. Klumpers,Sandeep T. Koshy,James C. Weaver,Ovijit Chaudhuri,Raquel Seruca,Fátima Carneiro,Pedro L. Granja,David J. Mooney,David J. Mooney +9 more
TL;DR: 3D cultures revealed the same CD44 alternative splicing pattern previously observed in human premalignant and malignant gastric lesions, indicating that fundamental features of 3D cultures - such as soluble factors diffusion and mechanical cues - influence CD44 expression in GC cells.