D
David Robinson
Researcher at United States Department of Energy
Publications - 6
Citations - 123
David Robinson is an academic researcher from United States Department of Energy. The author has contributed to research in topics: Cas9 & Genome editing. The author has an hindex of 3, co-authored 6 publications receiving 63 citations. Previous affiliations of David Robinson include Joint Genome Institute.
Papers
More filters
Journal ArticleDOI
CRAGE enables rapid activation of biosynthetic gene clusters in undomesticated bacteria.
Gaoyan Wang,Zhiying Zhao,Jing Ke,Yvonne Engel,Yi-Ming Shi,David Robinson,Kerem Bingol,Zheyun Zhang,Benjamin P. Bowen,Benjamin P. Bowen,Katherine B. Louie,Bing Wang,Robert Evans,Yu Miyamoto,Kelly Cheng,Suzanne M. Kosina,Markus de Raad,Leslie P. Silva,Alicia Luhrs,Andrea Lubbe,David W. Hoyt,Charles Francavilla,Hiroshi Otani,Hiroshi Otani,Samuel Deutsch,Samuel Deutsch,Nancy M. Washton,Edward M. Rubin,Nigel J. Mouncey,Nigel J. Mouncey,Axel Visel,Axel Visel,Trent R. Northen,Trent R. Northen,Jan Fang Cheng,Jan Fang Cheng,Helge B. Bode,Yasuo Yoshikuni +37 more
TL;DR: Development of chassis-independent recombinase-assisted genome engineering (CRAGE) enables the integration of plasmids encoding biosynthetic gene clusters into the chromosomes of diverse bacteria to optimize production of natural products in non-native strains.
Journal ArticleDOI
Exploiting members of the BAHD acyltransferase family to synthesize multiple hydroxycinnamate and benzoate conjugates in yeast
Aymerick Eudes,Aymerick Eudes,Maxence Mouille,Maxence Mouille,David Robinson,Veronica Teixeira Benites,Veronica Teixeira Benites,Veronica Teixeira Benites,George Wang,George Wang,Lucien Roux,Lucien Roux,Lucien Roux,Yi-Lin Tsai,Yi-Lin Tsai,Edward E. K. Baidoo,Edward E. K. Baidoo,Tsan-Yu Chiu,Tsan-Yu Chiu,Joshua L. Heazlewood,Joshua L. Heazlewood,Joshua L. Heazlewood,Henrik Vibe Scheller,Henrik Vibe Scheller,Aindrila Mukhopadhyay,Aindrila Mukhopadhyay,Jay D. Keasling,Samuel Deutsch,Dominique Loqué,Dominique Loqué,Dominique Loqué +30 more
TL;DR: The effectiveness of expressing members of the plant BAHD acyltransferase family in yeast for the synthesis of numerous valuable hydroxycinnamate and benzoate conjugates is demonstrated.
Journal ArticleDOI
Bacterial genome editing by coupling Cre-lox and CRISPR-Cas9 systems.
Hualan Liu,Hualan Liu,David Robinson,Zong-Yen Wu,Zong-Yen Wu,Rita Kuo,Yasuo Yoshikuni,Yasuo Yoshikuni,Ian K. Blaby,Ian K. Blaby,Jan Fang Cheng,Jan Fang Cheng +11 more
TL;DR: A landing pad that carries one wild-type and two mutant lox sites to allow integration of foreign DNA at two locations through Cre-lox recombinase-mediated cassette exchange is developed and its functionality across multiple species is demonstrated.
Journal ArticleDOI
An integrated workflow for phenazine-modifying enzyme characterization.
R. Cameron Coates,Benjamin P. Bowen,Benjamin P. Bowen,Ernst Oberortner,Linda S. Thomashow,Michalis Hadjithomas,Zhiying Zhao,Jing Ke,Leslie P. Silva,Katherine B. Louie,Gaoyan Wang,David Robinson,Angela Tarver,Matthew J. Hamilton,Andrea Lubbe,Meghan E. Feltcher,Jeffery L. Dangl,Amrita Pati,David M. Weller,Trent R. Northen,Trent R. Northen,Jan Fang Cheng,Jan Fang Cheng,Nigel J Mouncey,Samuel Deutsch,Samuel Deutsch,Yasuo Yoshikuni,Yasuo Yoshikuni +27 more
TL;DR: An integrated workflow for BGC characterization that integrates pathway identification, modular design, DNA synthesis, assembly and characterization is developed and demonstrated how this approach can accelerate functional characterization of BGCs.
Genome Editing in Escherichia coli with Cas9 and synthetic CRISPRs
TL;DR: A heat curable, serializable, plasmid based system for selectionless Cas9 editing in arbitrary E. coli strains that uses synthetic CRISPRs for targeting and -red to effect repairs of double stranded breaks is developed.