M
Markus de Raad
Researcher at Lawrence Berkeley National Laboratory
Publications - 35
Citations - 820
Markus de Raad is an academic researcher from Lawrence Berkeley National Laboratory. The author has contributed to research in topics: Mass spectrometry & Chemistry. The author has an hindex of 15, co-authored 29 publications receiving 564 citations. Previous affiliations of Markus de Raad include Joint Genome Institute & Utrecht University.
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Journal ArticleDOI
Lessons from Two Design-Build-Test-Learn Cycles of Dodecanol Production in Escherichia coli Aided by Machine Learning.
Paul Opgenorth,Paul Opgenorth,Zak Costello,Zak Costello,Takuya Okada,Garima Goyal,Garima Goyal,Yan Chen,Yan Chen,Jennifer W. Gin,Jennifer W. Gin,Veronica T. Benites,Veronica T. Benites,Markus de Raad,Markus de Raad,Trent R. Northen,Trent R. Northen,Trent R. Northen,Kai Deng,Samuel Deutsch,Edward E. K. Baidoo,Edward E. K. Baidoo,Christopher J. Petzold,Christopher J. Petzold,Nathan J. Hillson,Hector Garcia Martin,Harry R. Beller,Harry R. Beller +27 more
TL;DR: Findings from implementation of two DBTL cycles to optimize 1-dodecanol production from glucose using 60 engineered Escherichia coli MG1655 strains are reported on, including the importance of sequencing checks on plasmids in production strains as well as in cloning strains, and the critical need for more accurate protein expression predictive tools.
Journal ArticleDOI
CRAGE enables rapid activation of biosynthetic gene clusters in undomesticated bacteria.
Gaoyan Wang,Zhiying Zhao,Jing Ke,Yvonne Engel,Yi-Ming Shi,David Robinson,Kerem Bingol,Zheyun Zhang,Benjamin P. Bowen,Benjamin P. Bowen,Katherine B. Louie,Bing Wang,Robert Evans,Yu Miyamoto,Kelly Cheng,Suzanne M. Kosina,Markus de Raad,Leslie P. Silva,Alicia Luhrs,Andrea Lubbe,David W. Hoyt,Charles Francavilla,Hiroshi Otani,Hiroshi Otani,Samuel Deutsch,Samuel Deutsch,Nancy M. Washton,Edward M. Rubin,Nigel J. Mouncey,Nigel J. Mouncey,Axel Visel,Axel Visel,Trent R. Northen,Trent R. Northen,Jan Fang Cheng,Jan Fang Cheng,Helge B. Bode,Yasuo Yoshikuni +37 more
TL;DR: Development of chassis-independent recombinase-assisted genome engineering (CRAGE) enables the integration of plasmids encoding biosynthetic gene clusters into the chromosomes of diverse bacteria to optimize production of natural products in non-native strains.
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Production and biomedical applications of virus-like particles derived from polyomaviruses.
TL;DR: This review will highlight the structural characteristics of polyomavirus-derived VLPs and give an overview of their applications in diagnostics, vaccine development and gene delivery.
Journal ArticleDOI
High-throughput platforms for metabolomics
TL;DR: Promising emerging technologies, including microfluidics and miniaturization of separation techniques, have the potential to achieve both rapid and more comprehensive metabolite analysis.
Journal ArticleDOI
Function-Driven Single-Cell Genomics Uncovers Cellulose-Degrading Bacteria from the Rare Biosphere
Devin F. R. Doud,Robert M. Bowers,Frederik Schulz,Markus de Raad,Kai Deng,Kai Deng,Angela Tarver,Evan Glasgow,Evan Glasgow,Kirk A. Vander Meulen,Kirk A. Vander Meulen,Brian G. Fox,Brian G. Fox,Samuel Deutsch,Yasuo Yoshikuni,Yasuo Yoshikuni,Trent R. Northen,Trent R. Northen,Brian P. Hedlund,Steven W. Singer,Steven W. Singer,Natalia Ivanova,Natalia Ivanova,Tanja Woyke,Tanja Woyke,Tanja Woyke +25 more
TL;DR: To identify and characterize yet uncultivated microbial taxa involved in cellulose degradation, this work developed and benchmarked a function-driven single-cell screen, which was applied to a microbial community inhabiting the Great Boiling Spring Geothermal Field, northwest Nevada and is expected to be extended to a broad range of substrates.