Leslie P. Silva

TL;DR: A straightforward, three-step protocol for exosome isolation of cell culture supernatants or large volumes of biofluid based on sequential steps of dead-end pre-filtration, tangential flow filtration (TFF), and low-pressure track-etched membrane filTration that is easy-to-implement and amenable to translation for clinical exosomes isolations.

TL;DR: It is concluded that DNA concentration is a widely applicable method for normalizing metabolomic data from adherent cell lines.

TL;DR: Development of chassis-independent recombinase-assisted genome engineering (CRAGE) enables the integration of plasmids encoding biosynthetic gene clusters into the chromosomes of diverse bacteria to optimize production of natural products in non-native strains.

TL;DR: The sensitivity and accuracy of Gln, Glu, and pGlu quantitation by electrospray ionization-based mass spectrometry can be improved dramatically by using chromatographic conditions that adequately separate the three metabolites, isotopic internal standards to correct for in-source pGLU formation, and user-optimized fragmentor voltage for acquisition of the MS spectra.

TL;DR: Results provide the first biochemical evidence for a redox‐dependent structural switch that regulates SEX4 activity, which represents the first plant phosphatase known to undergo reversible oxidation via disulfide bond formation like its mammalian counterparts.