Debra Burdick

TL;DR: a4−/− mice exhibit a profound accumulation of iron in splenic macrophages, which are critical for the reutilization of iron from engulfed red blood cells, and a previously unappreciated role for autophagy and NCOA4 in the control of iron homeostasis in vivo is revealed.

TL;DR: In rodent models of hypertension, WNK463 affects blood pressure and body fluid and electro-lyte homeostasis, consistent with WNK-kinase-associated physiology and pathophysiology.

TL;DR: This work applied the latter approach to the With-No-Lysine (K) (WNK) kinases to discover lead molecules for a next-generation antihypertensive that requires a stringent safety profile, yielding several ATP noncompetitive WNK1-4 kinase inhibitors that inhibited rubidium uptake by sodium chloride cotransporter 1 in HT29 cells, consistent with the reported physiology of WNK kinases in renal electrolyte handling.

TL;DR: In this article, an affinity-based screen was employed to identify high-affinity PCSK9 ligands that utilize a unique, induced-fit pocket and partially disrupt the PCSK 9-LDLR interaction.

TL;DR: The use of the SelecT automated cell-culture platform is described herein in the authors' discovery and preclinical profiling programs at Novartis, and achieving the balance between cells and the automated environment, and accommodating variable cell dynamics are discussed.