Journal ArticleDOI
Expression of active human factor VIII from recombinant DNA clones
William I. Wood,Daniel J. Capon,Christian C. Simonsen,Dan L. Eaton,Jane Gitschier,Bruce Keyt,Peter H. Seeburg,Douglas H. Smith,P Hollingshead,Karen L. Wion,Eric Delwart,Edward G. D. Tuddenham,Gordon A. Vehar,Richard M. Lawn +13 more
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TLDR
The recombinant protein corrects the clotting time of plasma from haemophiliacs and has many of the biochemical and immunological characteristics of serum-derived factor VIII.Abstract:
DNA clones encoding the complete 2,351 amino acid sequence for human factor VIII have been isolated and used to produce biologically active factor VIII in cultured mammalian cells. The recombinant protein corrects the clotting time of plasma from haemophiliacs and has many of the biochemical and immunological characteristics of serum-derived factor VIII.read more
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Human tumour necrosis factor: precursor structure, expression and homology to lymphotoxin.
Diane Pennica,Glenn Evan Nedwin,Joel S. Hayflick,Peter H. Seeburg,Rik Derynck,Michael A. Palladino,William J. Kohr,Bharat B. Aggarwal,David V. Goeddel +8 more
TL;DR: Recombinant tumour necrosis factor can be obtained by expression of its complementary DNA in Escherichia coli and induces the haemorrhagic necrosis of transplanted methylcholanthrene-induced sarcomas in syngeneic mice.
Journal ArticleDOI
A parathyroid hormone-related protein implicated in malignant hypercalcemia: cloning and expression
Larry J. Suva,G. A. Winslow,Richard E.H. Wettenhall,R Hammonds,Jane M. Moseley,H. Diefenbach-Jagger,Christine Rodda,Bruce E. Kemp,H. Rodriguez,E. Y. Chen,P. J. Hudson,T. J. Martin,William I. Wood +12 more
TL;DR: The isolation of full-length complementary DNA clones of a putative hypercalcemia factor, and the expression from the cloned DNA of the active protein in mammalian cells that has significant homology with parathyroid hormone in the amino-terminal region are reported.
Journal ArticleDOI
A heparin-binding growth factor secreted by macrophage-like cells that is related to EGF.
TL;DR: This heparin-binding EGF-like growth factor (HB-EGF) binds to EGF receptors on A-431 epidermoid carcinoma cells and smooth muscle cells, but is a far more potent mitogen for smoother muscle cells than is EGF.
Journal ArticleDOI
Nucleotide sequence of a bovine clone encoding the angiogenic protein, basic fibroblast growth factor.
Judith A. Abraham,Ayalew Mergia,Jacqueline L. Whang,Annette Tumolo,Jeffrey M. Friedman,Kathryn A. Hjerrild,Denis Gospodarowicz,John C. Fiddes +7 more
TL;DR: An oligonucleotide probe was designed from the nucleotide sequence of the amino-terminal exon of bovine acidic FGF, taking into account the 55 percent amino acid sequence homology between the two factors.
Journal ArticleDOI
Characterization of the human factor VIII gene
Jane Gitschier,William I. Wood,Therese M. Goralka,Karen L. Wion,Ellson Y. Chen,Dennis H. Eaton,Gordon A. Vehar,Daniel J. Capon,Richard M. Lawn +8 more
TL;DR: The complete 186,000 base-pair (bp) human factor VIII gene has been isolated and consists of 26 exons ranging in size from 69 to 3,106 bp and introns as large as 32.4 kilobases as mentioned in this paper.
References
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Book
Molecular Cloning: A Laboratory Manual
TL;DR: Molecular Cloning has served as the foundation of technical expertise in labs worldwide for 30 years as mentioned in this paper and has been so popular, or so influential, that no other manual has been more widely used and influential.
Journal ArticleDOI
DNA sequencing with chain-terminating inhibitors
TL;DR: A new method for determining nucleotide sequences in DNA is described, which makes use of the 2',3'-dideoxy and arabinon nucleoside analogues of the normal deoxynucleoside triphosphates, which act as specific chain-terminating inhibitors of DNA polymerase.
Journal ArticleDOI
The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers.
Jeffrey Vieira,Joachim Messing +1 more
TL;DR: A series of plasmid vectors containing the multiple cloning site (MCS7) of M13mp7 has been constructed and a kanamycin-resistance marker has been inserted into the center of the symmetrical MCS7 to yield a restriction-site-mobilizing element (RSM).
Journal ArticleDOI
Organization and Expression of Eucaryotic Split Genes Coding for Proteins
R Breathnach,P Chambon +1 more
TL;DR: This paper organizes the organization of protein codes into split genes, a small number of which are expressed in the chickenuct, and discusses generalization, generalization and Molecular Evolution.
Expression of eucaryotic split genes coding for proteins
TL;DR: In this paper, the split genes were described as follows: Globin genes expressed in the chicken o,iduct, Vitellogenin genes, Collagen genes and Actin genes.