E
Elena M. Turek
Researcher at National Institutes of Health
Publications - 10
Citations - 2708
Elena M. Turek is an academic researcher from National Institutes of Health. The author has contributed to research in topics: Microbiome & Bronchiectasis. The author has an hindex of 4, co-authored 8 publications receiving 2145 citations. Previous affiliations of Elena M. Turek include Imperial College London.
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Journal ArticleDOI
Reagent and laboratory contamination can critically impact sequence-based microbiome analyses
Susannah J. Salter,Michael J. Cox,Elena M. Turek,Szymon T. Calus,William O.C.M. Cookson,Miriam F. Moffatt,Paul Turner,Paul Turner,Julian Parkhill,Nicholas J. Loman,Alan W. Walker,Alan W. Walker +11 more
TL;DR: It is demonstrated that contaminating DNA is ubiquitous in commonly used DNA extraction kits and other laboratory reagents, varies greatly in composition between different kits and kit batches, and that this contamination critically impacts results obtained from samples containing a low microbial biomass.
Journal ArticleDOI
Longitudinal assessment of sputum microbiome by sequencing of the 16S rRNA gene in non-cystic fibrosis bronchiectasis patients.
Michael J. Cox,Elena M. Turek,Catherine Hennessy,Ghazala K. Mirza,Phillip James,MC Coleman,Andrew M. Jones,Robert Wilson,Diana Bilton,William O.C.M. Cookson,Miriam F. Moffatt,Michael R. Loebinger +11 more
TL;DR: DNA based methods offer better identification and quantification of microbial constituents of sputum than standard clinical culture and may help inform patient management strategies, and microbial characteristics showed poor correlations with clinical features including underlying disease, antibiotic use and exacerbations.
Journal ArticleDOI
Inhaled corticosteroid suppression of cathelicidin drives dysbiosis and bacterial infection in chronic obstructive pulmonary disease
Aran Singanayagam,Nicholas Glanville,Leah Cuthbertson,Nathan W. Bartlett,Nathan W. Bartlett,Lydia J. Finney,Elena M. Turek,Eteri Bakhsoliani,Maria Adelaide Calderazzo,Maria-Belen Trujillo-Torralbo,Joseph Footitt,Phillip James,Peter Fenwick,Samuel V. Kemp,Thomas B. Clarke,Jadwiga A. Wedzicha,Michael R. Edwards,Miriam F. Moffatt,William O.C.M. Cookson,Patrick Mallia,Sebastian L. Johnston +20 more
TL;DR: It is shown that ICS use is associated with lung microbiota disruption leading to proliferation of streptococcal genera, an effect that could be recapitulated in ICS-treated mice, and a central role for cathepsin D/cathelicidin in the suppression of antibacterial host defense by ICS in COPD.
Posted ContentDOI
Reagent contamination can critically impact sequence-based microbiome analyses
Susannah J. Salter,Michael J. Cox,Elena M. Turek,Szymon T. Calus,William O.C.M. Cookson,Miriam F. Moffatt,Paul Turner,Julian Parkhill,Nicholas J. Loman,Alan W. Walker +9 more
TL;DR: It is demonstrated that contaminating DNA is ubiquitous in commonly used DNA extraction kits, varies greatly in composition between different kits and kit batches, and that this contamination critically impacts results obtained from samples containing a low microbial biomass.
Journal ArticleDOI
Airway microbial communities, smoking and asthma in a general population sample
Elena M. Turek,Michael J. Cox,Michael Hunter,Jennie Hui,Phillip James,Saffron A.G. Willis-Owen,Leah Cuthbertson,Alan L. James,A. William Musk,Miriam F. Moffatt,William O.C.M. Cookson +10 more
TL;DR: This article investigated airway bacterial communities in a general population sample of 529 Australian adults and found that the healthy airway microbiota in this population were contained within a highly structured ecosystem, suggesting balanced relationships between the microbiome and human host factors.