Emily M. Holloway

TL;DR: This work used homogeneous human bud tip organoid cultures and identified SMAD signaling as a key regulator of the bud tip-to-airway transition and shed light on human airway differentiation in vitro and provides a single-cell atlas of the developing human lung.

TL;DR: This work uses single-cell mRNA sequencing with in situ validation approaches to interrogate human intestinal development from 7-21 weeks post conception, assigning molecular identities and spatial locations to cells and factors that comprise the niche.

TL;DR: HIOs can co-differentiate a native EC population that is properly patterned with an intestine-specific EC transcriptional signature in vitro, and it is found that HIO ECs grown in vitro share the highest similarity with native intestinal ECs relative to kidney and lung.

TL;DR: Efforts to characterize human organ cellular complexity and attempts to make organoid models more realistic through co-culture, transplantation and bioengineering approaches are discussed.

TL;DR: In this article, the authors re-analyzed the transcriptional distinction between distal nephron and ureteric epithelium in human fetal kidney, and showed that the distal kidney segment alone displays significant in vitro plasticity and can adopt a uteric tip identity when isolated and cultured in defined conditions.