Enrico Gottardi

TL;DR: The standardized RT-PCR protocol and primer sets can now be used for molecular classification of acute leukemia at diagnosis and for MRD detection during follow-up to evaluate treatment effectiveness.

TL;DR: The ABL gene is proposed to be used as CG for RQ-PCR-based diagnosis and MRD detection in leukemic patients and these data are not only eligible for quantifying of fusion gene transcripts, but also for the quantification of aberrantly expressed genes.

TL;DR: Application of a standardized WT1 assay provides independent prognostic information in AML, lending support to incorporation of early assessment of MRD to develop more robust risk scores, to enhance risk stratification, and to identify patients who may benefit from allogeneic transplantation.

TL;DR: For most patients with imatinib resistance and with mutations, nilotinib offers a substantial probability of response, however, mutational status at baseline may influence response.

TL;DR: In this paper, the authors developed highly sensitive real-time quantitative (RQ) polymerase chain reaction (PCR) assays, either in DNA or RNA, that are specific for various NPM1 mutations.