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Anthonie Willem Langerak
Researcher at Erasmus University Rotterdam
Publications - 67
Citations - 7780
Anthonie Willem Langerak is an academic researcher from Erasmus University Rotterdam. The author has contributed to research in topics: Gene rearrangement & Platelet-derived growth factor receptor. The author has an hindex of 33, co-authored 67 publications receiving 7151 citations.
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Journal ArticleDOI
Design and standardization of PCR primers and protocols for detection of clonal immunoglobulin and T-cell receptor gene recombinations in suspect lymphoproliferations: report of the BIOMED-2 Concerted Action BMH4-CT98-3936.
J. J. M. Van Dongen,Anthonie Willem Langerak,Monika Brüggemann,Paul Evans,Michael Hummel,F. L. Lavender,Eric Delabesse,Frederic Davi,Ed Schuuring,Ramón García-Sanz,J.H.J.M. van Krieken,J Droese,David Gonzalez,Christian Bastard,Helen E. White,Marcel Spaargaren,Marcos González,A Parreira,John L. Smith,Gareth J. Morgan,Michael Kneba,Elisabeth Macintyre +21 more
TL;DR: The BIOMED-2 multiplex tubes can now be used for diagnostic clonality studies as well as for the identification of PCR targets suitable for the detection of minimal residual disease.
Journal ArticleDOI
Standardized RT-PCR analysis of fusion gene transcripts from chromosome aberrations in acute leukemia for detection of minimal residual disease. Report of the BIOMED-1 Concerted Action: investigation of minimal residual disease in acute leukemia.
van Dongen Jj,Elizabeth Macintyre,Jean Gabert,Eric Delabesse,Rossi,Giuseppe Saglio,Enrico Gottardi,Alessandro Rambaldi,Gianpietro Dotti,Frank Griesinger,A Parreira,Paula Gameiro,Diáz Mg,Maria Malec,Anthonie Willem Langerak,San Miguel Jf,Andrea Biondi +16 more
TL;DR: The standardized RT-PCR protocol and primer sets can now be used for molecular classification of acute leukemia at diagnosis and for MRD detection during follow-up to evaluate treatment effectiveness.
Journal ArticleDOI
EuroFlow antibody panels for standardized n-dimensional flow cytometric immunophenotyping of normal, reactive and malignant leukocytes.
J. J. M. Van Dongen,Ludovic Lhermitte,S Böttcher,Julia Almeida,V H J van der Velden,Juan Flores-Montero,Andy C. Rawstron,Vahid Asnafi,Quentin Lecrevisse,Paulo Sérgio Lucio,Ester Mejstrikova,Tomasz Szczepański,Tomas Kalina,R de Tute,Monika Brüggemann,Lukasz Sedek,M Cullen,Anthonie Willem Langerak,Alexandre de Mendonça,Elizabeth Macintyre,Marta Martin-Ayuso,Ondrej Hrusak,M B Vidriales,Alberto Orfao +23 more
TL;DR: The EuroFlow studies resulted in validated and flexible 8-color antibody panels for multidimensional identification and characterization of normal and aberrant cells, optimally suited for immunophenotypic screening and classification of hematological malignancies.
Journal ArticleDOI
EuroClonality/BIOMED-2 guidelines for interpretation and reporting of Ig/TCR clonality testing in suspected lymphoproliferations
Anthonie Willem Langerak,Patricia J. T. A. Groenen,Monika Brüggemann,Kheira Beldjord,Cristiana Bellan,Lisa Bonello,Elke Boone,G. I. Carter,Mark Catherwood,Frederic Davi,M. H. Delfau-Larue,Timothy C. Diss,Paul Evans,Paula Gameiro,R. García Sanz,David Gonzalez,D. Grand,A. Håkansson,Michael Hummel,Hongxiang Liu,L. Lombardia,Elizabeth Macintyre,BJ Milner,Santiago Montes-Moreno,Ed Schuuring,Marcel Spaargaren,Elizabeth Hodges,J. J. M. Van Dongen +27 more
TL;DR: Collectively, the EuroClonality (BIOMED-2) guidelines and consensus reporting system should help to improve the general performance level of clonality assessment and interpretation, which will directly impact on routine clinical management in patients with suspected lymphoproliferations.
Journal ArticleDOI
Heteroduplex PCR analysis of rearranged T cell receptor genes for clonality assessment in suspect T cell proliferations
Anthonie Willem Langerak,Tomasz Szczepański,M.E.L. van der Burg,Ingrid L. M. Wolvers-Tettero,J. J. M. Van Dongen +4 more
TL;DR: Heteroduplex PCR analysis of TCR gene rearrangements is a simple, rapid and cheap alternative to Southern blot analysis for detection of clonally rearranged TCR genes.