G
Gary J. Sarkis
Publications - 10
Citations - 9710
Gary J. Sarkis is an academic researcher. The author has contributed to research in topics: Nucleic acid & Hybrid genome assembly. The author has an hindex of 7, co-authored 10 publications receiving 9491 citations.
Papers
More filters
Journal ArticleDOI
Genome sequencing in microfabricated high-density picolitre reactors
Marcel Margulies,Michael Egholm,William E. Altman,Said Attiya,Joel S. Bader,Lisa A. Bemben,Jan Berka,Michael S. Braverman,Yi-Ju Chen,Zhoutao Chen,Scott Dewell,Lei Du,J. M. Fierro,Xavier V. Gomes,Brian C. Godwin,Wen He,Scott Edward Helgesen,Chun Heen Ho,Gerard P. Irzyk,Szilveszter C. Jando,Maria L. I. Alenquer,Thomas P. Jarvie,Kshama B. Jirage,Jong-Bum Kim,James R. Knight,Janna R. Lanza,John H. Leamon,Steven Lefkowitz,Ming Lei,Jing Li,Kenton Lohman,Hong Lu,Vinod Makhijani,Keith Mcdade,Michael P. McKenna,Eugene W. Myers,Elizabeth Nickerson,John Nobile,Ramona Plant,Bernard P. Puc,Michael T. Ronan,George T. Roth,Gary J. Sarkis,Jan Fredrik Simons,John Simpson,Maithreyan Srinivasan,Karrie R. Tartaro,Alexander Tomasz,Kari A. Vogt,Greg A. Volkmer,Shally H. Wang,Yong Wang,Michael P. Weiner,Pengguang Yu,Richard F. Begley,Jonathan M. Rothberg +55 more
TL;DR: A scalable, highly parallel sequencing system with raw throughput significantly greater than that of state-of-the-art capillary electrophoresis instruments with 96% coverage at 99.96% accuracy in one run of the machine is described.
Patent
Bead emulsion nucleic acid amplification
Jan Berka,Yi-Ju Chen,John H. Leamon,Steven Lefkowitz,Kenton Lohman,Vinod Makhijani,Jonathan M. Rothberg,Gary J. Sarkis,Maithreyan Srinivasan,Michael P. Weiner +9 more
TL;DR: In this paper, a method for nucleic acid amplification is described, in which the templates, beads, and amplification reaction solution are emulsified and the nucleic acids are amplified to provide clonal copies of the templates attached to the beads.
Journal ArticleDOI
Assessment of whole genome amplification-induced bias through high-throughput, massively parallel whole genome sequencing
Robert Pinard,Alex de Winter,Gary J. Sarkis,Mark Gerstein,Karrie R. Tartaro,Ramona Plant,Michael Egholm,Jonathan M. Rothberg,John H. Leamon +8 more
TL;DR: Of the amplification methodologies examined in this paper, the multiple displacement amplification products generated the least bias, and produced significantly higher yields of amplified DNA.
Journal ArticleDOI
A massively parallel PicoTiterPlate based platform for discrete picoliter-scale polymerase chain reactions.
John H. Leamon,William Lun Lee,Karrie R. Tartaro,Janna R. Lanza,Gary J. Sarkis,Alex D. deWinter,Jan Berka,Kenton Lohman +7 more
TL;DR: The PicoTiterPlate as discussed by the authors is a platform for simultaneous polymerase chain reaction (PCR) amplification of up to 300,000 discrete reactions in a novel platform, which can be performed in extremely small volumes: individual reactions volumes are as low as 39.5 pL, with a total 15.3 micro-L reaction volume for the entire platform.
Patent
Paired end sequencing
Jan Berka,Zhoutao Chen,Michael Egholm,Brian C. Godwin,Stephen K. Hutchison,John H. Leamon,Gary J. Sarkis,Jan Fredrik Simons +7 more
TL;DR: In this article, the authors proposed a method of preparing a target nucleic acid fragments to produce a smaller nucleic acids which comprises the two ends of the target nucleIC acid.