G
Gert Weber
Researcher at Free University of Berlin
Publications - 45
Citations - 3139
Gert Weber is an academic researcher from Free University of Berlin. The author has contributed to research in topics: RNA splicing & Spliceosome. The author has an hindex of 21, co-authored 36 publications receiving 2665 citations. Previous affiliations of Gert Weber include Helmholtz-Zentrum Berlin & Humboldt University of Berlin.
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Journal ArticleDOI
Helical extension of the neuronal SNARE complex into the membrane.
TL;DR: The results suggest that the final phase of SNARE assembly is directly coupled to membrane merger, and this structure shows that assembly proceeds beyond the already known core SNARE complex, resulting in a continuous helical bundle that is further stabilized by side-chain interactions in the linker region.
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A reversibly photoswitchable GFP-like protein with fluorescence excitation decoupled from switching
Tanja Brakemann,Andre C. Stiel,Gert Weber,Martin Andresen,Ilaria Testa,Tim Grotjohann,Marcel Leutenegger,Uwe Plessmann,Henning Urlaub,Christian Eggeling,Markus C. Wahl,Stefan W. Hell,Stefan Jakobs +12 more
TL;DR: A bright, monomeric, reversibly photoswitchable variant of GFP, Dreiklang, whose fluorescence excitation spectrum is decoupled from that for optical switching, enabling far-field fluorescence nanoscopy in living mammalian cells using both a coordinate-targeted and a stochastic single molecule switching approach.
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Structural basis for reversible photoswitching in Dronpa
Martin Andresen,Andre C. Stiel,Simon Trowitzsch,Gert Weber,Christian Eggeling,Markus C. Wahl,Stefan W. Hell,Stefan Jakobs +7 more
TL;DR: A comprehensive model for the light-induced switching mechanism is suggested, connecting a cascade of structural rearrangements with different protonation states of the chromophore with the aim of determining the elusive dark-state structure of Dronpa.
Journal ArticleDOI
Structure and mechanism of the reversible photoswitch of a fluorescent protein
Martin Andresen,Markus C. Wahl,Andre C. Stiel,Frauke Gräter,Lars V. Schäfer,Simon Trowitzsch,Gert Weber,Christian Eggeling,Helmut Grubmüller,Stefan W. Hell,Stefan Jakobs +10 more
TL;DR: The molecular photoswitching mechanism of asFP595, a green fluorescent protein (GFP)-like protein that can be transferred from a nonfluorescent "off" to a fluorescent "on" state and back again, is clarified by green and blue light, respectively.
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U1 small nuclear ribonucleoprotein immune complexes induce type I interferon in plasmacytoid dendritic cells through TLR7.
Emina Savarese,Ohk-wha Chae,Simon Trowitzsch,Gert Weber,Berthold Kastner,Shizuo Akira,Hermann Wagner,Roland M. Schmid,Stefan Bauer,Anne Krug +9 more
TL;DR: It is shown that murine PDCs are activated by purified U1snRNP/anti-Sm ICs to produce IFN-alpha and proinflammatory cytokines and to up-regulate costimulatory molecules.