Tim Grotjohann

TL;DR: An optical nanoscopy that records raw data images from living cells and tissues with low levels of light is demonstrated, facilitated by the generation of reversibly switchable enhanced green fluorescent protein (rsEGFP), a fluorescent protein that can be reversibly photoswitched more than a thousand times.

TL;DR: A bright, monomeric, reversibly photoswitchable variant of GFP, Dreiklang, whose fluorescence excitation spectrum is decoupled from that for optical switching, enabling far-field fluorescence nanoscopy in living mammalian cells using both a coordinate-targeted and a stochastic single molecule switching approach.

TL;DR: This work shows that nanoscopy based on the principle called RESOLFT (reversible saturable optical fluorescence transitions) or nonlinear structured illumination can be effectively parallelized using two incoherently superimposed orthogonal standing light waves, providing isotropic resolution in the focal plane and making pattern rotation redundant.

TL;DR: The generation of rsEGFP2 providing faster switching and the use of this protein to demonstrate 25–250 times faster recordings are reported on.

TL;DR: The development of robustly photoswitchable variants of enhanced green fluorescent protein (EGFP), named rsGreens, that display up to 30-fold higher fluorescence in E. coli colonies grown at 37 °C and more than 4-foldHigher fluorescence when expressed in HEK293T cells compared to their ancestor protein rsEGFP are presented.