G
Graeme Milligan
Researcher at University of Glasgow
Publications - 570
Citations - 32250
Graeme Milligan is an academic researcher from University of Glasgow. The author has contributed to research in topics: Receptor & G protein. The author has an hindex of 88, co-authored 556 publications receiving 30032 citations. Previous affiliations of Graeme Milligan include University of Leicester & Autonomous University of Barcelona.
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Journal ArticleDOI
Mechanism of Action of Gq to Inhibit Gβγ Modulation of CaV2.2 Calcium Channels: Probed by the Use of Receptor-Gα Tandems
TL;DR: In this paper, the stable interaction of a G-protein coupled receptor and a particular partner G protein was made possible by creating tandems between the alpha(2A)-R-G alpha and pertussis toxin-resistant mutants of different G alpha subunits of heterotrimeric Gproteins.
Book ChapterDOI
Specificity and functional applications of antipeptide antisera which identify G-protein alpha subunits.
TL;DR: It became clear that more selective tools would be required to discriminate among receptor functions transmitted to effector moieties by the individual G proteins.
Journal ArticleDOI
Novel mutation in the AVPR2 gene in a Danish male with nephrogenic diabetes insipidus caused by ER retention and subsequent lysosomal degradation of the mutant receptor
Lene N. Nejsum,Tomas Møller Christensen,Joris H. Robben,Graeme Milligan,Peter M.T. Deen,Daniel G. Bichet,Klaus Levin,Klaus Levin +7 more
TL;DR: Analysis of the mutant receptor in Madin-Darby Canine Kidney cell culture revealed that AVPR2-L170P was retained in the endoplasmic reticulum, and the expression was dramatically downregulated compared to wild-type AV PR2.
Journal ArticleDOI
Stimulation of high-affinity GTPase activity and cholera toxin-catalysed [32P]ADP-ribosylation of Gi by lysophosphatidic acid (LPA) in wild-type and α2C10 adrenoceptor-transfected Rat 1 fibroblasts
TL;DR: The results presented herein demonstrate conclusively that the pertussis toxin-sensitive effects of LPA in Rat 1 fibroblasts and a clone of these cells expressing the alpha 2C10 adrenoceptor are produced directly by the activation of Gi.
Journal ArticleDOI
Heterodimerisation of G protein-coupled receptors: implications for drug design and ligand screening
TL;DR: This review gives an overview of new strategies that have been developed in an effort to incorporate the possibilities added by GPCR hetero-oligomerisation on the screening of compounds as drug candidates.