G
Graeme Milligan
Researcher at University of Glasgow
Publications - 570
Citations - 32250
Graeme Milligan is an academic researcher from University of Glasgow. The author has contributed to research in topics: Receptor & G protein. The author has an hindex of 88, co-authored 556 publications receiving 30032 citations. Previous affiliations of Graeme Milligan include University of Leicester & Autonomous University of Barcelona.
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Journal ArticleDOI
Periplakin interferes with G protein activation by the melanin-concentrating hormone receptor-1 by binding to the proximal segment of the receptor C-terminal tail.
Hannah Murdoch,Gui Jie Feng,Dietmar Bächner,Laura Ormiston,Julia H. White,Dietmar Richter,Graeme Milligan +6 more
TL;DR: Periplakin is the first protein described to modify the capacity of the melanin-concentrating hormone-1 receptor to initiate signal transduction.
Journal ArticleDOI
Agonist regulation of adenylate cyclase activity in neuroblastoma x glioma hybrid NG108-15 cells transfected to co-express adenylate cyclase type II and the beta 2-adrenoceptor. Evidence that adenylate cyclase is the limiting component for receptor-mediated stimulation of adenylate cyclase activity.
TL;DR: It is demonstrated that the maximal output of the stimulatory arm of theAdenylate cyclase cascade can be increased by increasing total levels of adenylates cyclase in the genetic background of NG108-15 cells.
Journal Article
Thyrotropin-releasing hormone-induced subcellular redistribution and down-regulation of G11alpha: analysis of agonist regulation of coexpressed G11alpha species variants.
TL;DR: Using this system, both agonist-induced subcellular redistribution and down-regulation of G11alpha and beta subunit proteins in response to activation of a phospholipase C coupled receptor are demonstrated.
Journal ArticleDOI
G Protein-Coupled Receptor Fusion Proteins in Drug Discovery
Graeme Milligan,Gui Jie Feng,Richard J. Ward,Nana Sartania,Douglas Ramsay,Alison J. McLean,Juan J. Carrillo +6 more
TL;DR: Introduction of N-terminal tags to receptors has also allowed the introduction of novel assay techniques based on a pH-sensitive cyanine dye that have the capacity to overcome certain limitations of GPCR-fluorescent protein fusions.
Journal ArticleDOI
The palmitoylation status of the G-protein G(o)1 alpha regulates its activity of interaction with the plasma membrane.
Morag A. Grassie,J F McCallum,Francesca Guzzi,Anthony I. Magee,Graeme Milligan,Marco Parenti +5 more
TL;DR: Plasmids containing cDNAs encoding either the wild-type guanine-nucleotide-binding protein G(o)1 alpha or the palmitoylation-negative cysteine-3-to-serine (C3S) mutant of G(i)1alpha were transfected into Rat 1 cells, and clones stably expressing immunoreactivity corresponding to these polypeptides were isolated.