G
Greg L. Challis
Researcher at University of Warwick
Publications - 4
Citations - 2258
Greg L. Challis is an academic researcher from University of Warwick. The author has contributed to research in topics: Gene & Streptomyces coelicolor. The author has an hindex of 4, co-authored 4 publications receiving 2140 citations.
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Journal ArticleDOI
PCR-targeted Streptomyces gene replacement identifies a protein domain needed for biosynthesis of the sesquiterpene soil odor geosmin
TL;DR: An efficient procedure for creating precise gene replacements in the cosmid clones by using PCR targeting and λ-Red-mediated recombination is described, which is used successfully by >20 researchers to mutate around 100 Streptomyces genes.
Journal ArticleDOI
Genomic plasticity of the causative agent of melioidosis, Burkholderia pseudomallei
Matthew T. G. Holden,Richard W. Titball,Richard W. Titball,Sharon J. Peacock,Sharon J. Peacock,Ana Cerdeño-Tárraga,Timothy P. Atkins,Lisa Crossman,Tyrone Pitt,Carol Churcher,Karen Mungall,Stephen D. Bentley,Mohammed Sebaihia,Nicholas R. Thomson,Nathalie Bason,Ifor R. Beacham,Karen Brooks,Katherine A. Brown,Nat F. Brown,Greg L. Challis,Inna Cherevach,Tracy Chillingworth,Ann Cronin,Ben Crossett,Paul Davis,David DeShazer,Theresa Feltwell,Audrey Fraser,Zahra Hance,Heidi Hauser,S. Holroyd,Kay Jagels,Karen E. Keith,Mark Maddison,Sharon Moule,Claire Price,Michael A. Quail,Ester Rabbinowitsch,Kim Rutherford,Mandy Sanders,Mark Simmonds,Sirirurg Songsivilai,K. Stevens,Sarinna Tumapa,Monkgol Vesaratchavest,Sally Whitehead,Corin Yeats,Bart Barrell,Petra C. F. Oyston,Julian Parkhill +49 more
TL;DR: It is proposed that variable horizontal gene acquisition by B. pseudomallei is an important feature of recent genetic evolution and that this has resulted in a genetically diverse pathogenic species.
Journal ArticleDOI
Extracellular signalling, translational control, two repressors and an activator all contribute to the regulation of methylenomycin production in Streptomyces coelicolor
Sean O'Rourke,Andreas Wietzorrek,Kay Fowler,Christophe Corre,Greg L. Challis,Keith F. Chater +5 more
TL;DR: Genetic evidence suggests that these two genes encode components of a hetero‐oligomeric repressor of MMF and Mm biosynthesis, which is shown to account for the ability of certain Mm non‐producing mutants to act as ‘secretors’ in cosynthesis with other ‘convertor’ mutants.
Journal ArticleDOI
The vbs genes that direct synthesis of the siderophore vicibactin in Rhizobium leguminosarum: Their expression in other genera requires ECF σ factor rpoI
R. A. Carter,P. S. Worsley,Gary Sawers,Greg L. Challis,Michael J. Dilworth,Kerry C. Carson,J. A. Lawrence,Margaret Wexler,Andrew W. B. Johnston,Kay H. Yeoman +9 more
TL;DR: A biochemical genetic model of vicibactin synthesis is presented, which accommodates the phenotypes of different vbs mutants and the homologies of the vbs gene products.