Harry J. Mangalam

TL;DR: The structure of Pit-1 and its recognition elements suggests that metazoan tissue phenotype is controlled by a family of transcription factors that bind to related cis-active elements and contain several highly conserved domains.

TL;DR: It is found that when the pituitary-specific 33-kD transcription factor Pit-1 is expressed in either the heterologous HeLa cell line or in bacteria, it binds to and activates transcription from both growth hormone and prolactin promoters in vitro at levels even 10-fold lower than those normally present in pituitaries.

TL;DR: A model is proposed suggesting that both epidermal growth factor and phorbol esters stimulate the transcription of responsive genes via discrete classes of hormone-dependent, enhancer-like elements that bind different trans-acting factors, even in the absence of hormone stimulation.

TL;DR: A large, highly related POU-domain gene family, potentially exceeding 100 members, has been conserved and expanded in evolution to meet the increasing requirements for more intricate patterns of cell phenotypes.

TL;DR: The multi-factorial nature of this regulation provides the possibility for a more restricted pattern of gene expression than that exhibited by the cognate trans-acting transcription factors.