H
Hermen S. Overkleeft
Researcher at Leiden University
Publications - 91
Citations - 3599
Hermen S. Overkleeft is an academic researcher from Leiden University. The author has contributed to research in topics: Antigen & MHC class I. The author has an hindex of 30, co-authored 91 publications receiving 3080 citations. Previous affiliations of Hermen S. Overkleeft include Technical University of Berlin & Netherlands Cancer Institute.
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Journal ArticleDOI
Intracellular bacterial growth is controlled by a kinase network around PKB/AKT1
Coenraad Kuijl,Nigel D. L. Savage,Marije Marsman,Adriaan W. Tuin,Lennert Janssen,David A. Egan,Mirjam Ketema,Rian van den Nieuwendijk,Susan J. F. van den Eeden,Annemieke Geluk,Alex Poot,Gijs A. van der Marel,Roderick L. Beijersbergen,Hermen S. Overkleeft,Tom H. M. Ottenhoff,Jacques Neefjes +15 more
TL;DR: Using a reciprocal chemical genetics approach, kinase inhibitors with antibiotic properties and their host targets are identified, and host signalling networks that are activated by intracellular bacteria for survival are determined.
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Pharmacological inhibition of glucosylceramide synthase enhances insulin sensitivity
Johannes M. F. G. Aerts,Roelof Ottenhoff,Andrew S. Powlson,Aldo Grefhorst,Marco van Eijk,Peter F. Dubbelhuis,Jan Aten,Folkert Kuipers,Mireille J. Serlie,Tom Wennekes,Jaswinder K. Sethi,Stephen O'Rahilly,Hermen S. Overkleeft +12 more
TL;DR: In cultured 3T3-L1 adipocytes, the iminosugar derivative N-(5′-adamantane-1′-yl-methoxy)-pentyl-1-deoxynojirimycin (AMP-DNM) counteracted tumor necrosis factor-α–induced abnormalities in glycosphingolipid concentrations and concomitantly reversed abnormalities in insulin signal transduction.
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Discovery of an essential nucleotidylating activity associated with a newly delineated conserved domain in the RNA polymerase-containing protein of all nidoviruses
Kathleen C. Lehmann,Anastasia A. Gulyaeva,Jessika C. Zevenhoven-Dobbe,George M.C. Janssen,Mark Ruben,Hermen S. Overkleeft,Peter A. van Veelen,Dmitry V. Samborskiy,Alexander A. Kravchenko,Andrey M. Leontovich,Igor A. Sidorov,Eric J. Snijder,Clara C. Posthuma,Alexander E. Gorbalenya,Alexander E. Gorbalenya +14 more
TL;DR: Bioinformatics analysis of non-structural protein 9 of the arterivirus equine arteritis virus revealed a nidoviral signature domain (genetic marker) that is N-terminally adjacent to the RdRp and has no apparent homologs elsewhere, and is proposed to have nucleotidylation activity.
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Distinct uptake mechanisms but similar intracellular processing of two different toll-like receptor ligand-peptide conjugates in dendritic cells.
Selina Khan,Martijn S. Bijker,Jimmy J. Weterings,Hans J. Tanke,Gosse J. Adema,Thorbald van Hall,Jan W. Drijfhout,Cornelis J. M. Melief,Hermen S. Overkleeft,Gijsbert A. van der Marel,Dmitri V. Filippov,Sjoerd H. van der Burg,Ferry Ossendorp +12 more
TL;DR: The data show that targeting to two distinct TLRs requires distinct uptake mechanism but follows similar trafficking and intracellular processing pathways leading to optimal antigen presentation and T-cell priming.
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Identification of the Non-lysosomal Glucosylceramidase as β-Glucosidase 2
Rolf G. Boot,Marri Verhoek,Wilma E. Donker-Koopman,Anneke Strijland,Jan van Marle,Hermen S. Overkleeft,Tom Wennekes,Johannes M. F. G. Aerts +7 more
TL;DR: GBA2 plays a role in cellular glucosylceramide metabolism, and its activity is linked to sphingomyelin generation, and Hydrophobic deoxynojirimycins are extremely potent inhibitors for GBA2.