Hiroshi Hama

TL;DR: Time-lapse imaging is performed to explore the spatiotemporal patterns of cell-cycle dynamics during the epithelial-mesenchymal transition of cultured cells, the migration and differentiation of neural progenitors in brain slices, and the development of tumors across blood vessels in live mice.

TL;DR: In Scale-treated mouse brain, neurons labeled with genetically encoded fluorescent proteins were visualized at an unprecedented depth in millimeter-scale networks and at subcellular resolution, suggesting that the Scale method will be useful for light microscopy–based connectomics of cellular networks in brain and other tissues.

TL;DR: A gene encoding a fluorescent protein from a stony coral, Trachyphyllia geoffroyi, which emits green, yellow, and red light, is cloned, finding that the green-red conversion is highly sensitive to irradiation with UV or violet light, which excites the protonated form of the chromophore.

TL;DR: ScaleS permitted optical reconstructions of aged and diseased brain in Alzheimer's disease models, including mapping of 3D networks of amyloid plaques, neurons and microglia, and multi-scale tracking of single plaques by successive fluorescence and electron microscopy.

TL;DR: Evidence that astrocytes affect neuronal synaptogenesis by the process of adhesion is provided and propagation of PKC signaling represents an underlying mechanism for global neuronal maturation following localAstrocyte adhesion.