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Hongguang Huo
Researcher at Harvard University
Publications - Â 12
Citations - Â 7409
Hongguang Huo is an academic researcher from Harvard University. The author has contributed to research in topics: Induced pluripotent stem cell & Embryonic stem cell. The author has an hindex of 9, co-authored 12 publications receiving 7127 citations. Previous affiliations of Hongguang Huo include Boston Children's Hospital & Howard Hughes Medical Institute.
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Journal ArticleDOI
Reprogramming of human somatic cells to pluripotency with defined factors
In-Hyun Park,In-Hyun Park,Rui Zhao,Rui Zhao,Jason A. West,Jason A. West,Akiko Yabuuchi,Akiko Yabuuchi,Hongguang Huo,Hongguang Huo,Tan A. Ince,Paul H. Lerou,M. William Lensch,M. William Lensch,George Q. Daley,George Q. Daley +15 more
TL;DR: The data demonstrate that defined factors can reprogramme human cells to pluripotency, and establish a method whereby patient-specific cells might be established in culture.
Journal ArticleDOI
Disease-Specific Induced Pluripotent Stem Cells
In-Hyun Park,Natasha Arora,Hongguang Huo,Nimet Maherali,Tim Ahfeldt,Tim Ahfeldt,Akiko Shimamura,M. William Lensch,M. William Lensch,Chad A. Cowan,Konrad Hochedlinger,George Q. Daley +11 more
TL;DR: The generation of induced pluripotent stem cells from patients with a variety of genetic diseases with either Mendelian or complex inheritance are described, offering an unprecedented opportunity to recapitulate both normal and pathologic human tissue formation in vitro, thereby enabling disease investigation and drug development.
Journal ArticleDOI
Generation of induced pluripotent stem cells from human blood
Yuin-Han Loh,Yuin-Han Loh,Suneet Agarwal,Suneet Agarwal,In-Hyun Park,In-Hyun Park,Achia Urbach,Achia Urbach,Hongguang Huo,Hongguang Huo,Garrett C. Heffner,Garrett C. Heffner,Kitai Kim,Kitai Kim,Justine D. Miller,Justine D. Miller,Kitwa Ng,Kitwa Ng,George Q. Daley +18 more
TL;DR: The ability to reprogram cells from human blood will allow the generation of patient-specific stem cells for diseases in which the disease-causing somatic mutations are restricted to cells of the hematopoietic lineage.
Journal ArticleDOI
Live cell imaging distinguishes bona fide human iPS cells from partially reprogrammed cells
Elayne M. Chan,Sutheera Ratanasirintrawoot,Sutheera Ratanasirintrawoot,In-Hyun Park,Philip D. Manos,Philip D. Manos,Yuin-Han Loh,Hongguang Huo,Hongguang Huo,Justine D. Miller,Justine D. Miller,Odelya Hartung,Odelya Hartung,Junsung Rho,Tan A. Ince,George Q. Daley,Thorsten M. Schlaeger,Thorsten M. Schlaeger +17 more
TL;DR: Using serial live imaging of human fibroblasts undergoing reprogramming to identify distinct colony types that morphologically resemble embryonic stem cells yet differ in molecular phenotype and differentiation potential, it is determined that only one colony type represents true iPS cells, whereas the others represent reprograming intermediates.
Journal ArticleDOI
Generation of human-induced pluripotent stem cells
TL;DR: This protocol describes how to establish primary human fibroblasts lines and how to derive iPS cells by retroviral transduction of reprogramming factors, and takes 2 months to complete reprograming human primary fibro Blasts starting from biopsy.