Huan Gao

TL;DR: Findings from a whole-genome sequencing study of 88 matched HCC tumor/normal pairs, 81 of which are Hepatitis B virus (HBV) positive, find beta-catenin to be the mostrequently mutated oncogene and TP53 the most frequently mutated tumor suppressor.

TL;DR: Integrative analysis identified a pattern of paired intra-chromosomal translocations flanking focal amplifications and asymmetrical patterns of copy number variation flanking breakpoints of translocations in HCC.

TL;DR: In this article, a gene expression quantification analysis method comprising the steps that: mRNA is purified from total RNA, such that fragmented mRNA is prepared, the fragmented mRNA was subjected to reverse transcription, such as cDNA is prepared; the cDNA was purified, and the blunt-end DNA was prepared into a DNA fragment with an A base added at the end.

TL;DR: Disclosed as discussed by the authors is a method for analyzing quantification of gene expression, which consists of purifying mRNA from total RNA to prepare fragmented mRNA; reverse transcription of the fragmented mRNA to prepare cDNA, purifying the cDNA to prepare blunt end DNA and then purising the blunt-end DNA fragments; and adding connector sequences to both ends of the DNA fragments with an "A" base added at an end thereof.