Huang Wang

TL;DR: The core Protein Production Platform of the Northeast Structural Genomics Consortium (NESG) is described and the strategies used for producing high-quality protein samples are outlined, centered on the cloning, expression and purification of 6X-His-tagged proteins using T7-based Escherichia coli systems.

TL;DR: It is reported that a disease-relatedCOQ9 mutation leads to extensive disruption of the CoQ protein biosynthetic complex in a mouse model, and that COQ9 specifically interacts with COQ7 through a series of conserved residues.

TL;DR: It is demonstrated that human high mobility group box 1 (HMGB1) is a novel SA-binding protein, firmly establishing that SA binding to HMGB1 directly suppresses its proinflammatory activities.

TL;DR: This chapter describes the entire pipeline for producing isotope-enriched protein samples, and provides an in-depth description of recent advances in the production of NMR samples and their synergistic use with recent advancements in NMR hardware.

TL;DR: This Communication describes integration of PRE and DEER data with conventional solution-state nuclear magnetic resonance (NMR) methods for structure determination of Dsy0195, a homodimer from Desulfitobacterium hafniense and indicates that combination of conventional NMR restraints with only one or a few DEER distance constraints is sufficient for the automatic NMR-based structure determination program CYANA to build a network of interchain nuclear Overhauser effect constraints.