J
Jonathan A. Stefely
Researcher at University of Wisconsin-Madison
Publications - 28
Citations - 1371
Jonathan A. Stefely is an academic researcher from University of Wisconsin-Madison. The author has contributed to research in topics: Medicine & Biology. The author has an hindex of 14, co-authored 24 publications receiving 1014 citations. Previous affiliations of Jonathan A. Stefely include Harvard University & University of Cincinnati.
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Journal ArticleDOI
Mitochondrial Protein Interaction Mapping Identifies Regulators of Respiratory Chain Function
Brendan J. Floyd,Emily M. Wilkerson,Mike T. Veling,Mike T. Veling,Catie E. Minogue,Chuanwu Xia,Emily T. Beebe,Russell L. Wrobel,Holly Cho,Holly Cho,Laura S. Kremer,Charlotte L. Alston,Katarzyna A. Gromek,Brendan K. Dolan,Arne Ulbrich,Jonathan A. Stefely,Jonathan A. Stefely,Sarah L. Bohl,Sarah L. Bohl,Kelly M. Werner,Adam Jochem,Michael S. Westphall,Jarred W. Rensvold,Robert W. Taylor,Holger Prokisch,Jung-Ja P. Kim,Joshua J. Coon,David J. Pagliarini,David J. Pagliarini +28 more
TL;DR: It is discovered that LYRM5 interacts with and deflavinates the electron-transferring flavoprotein that shuttles electrons to coenzyme Q (CoQ) and a dynamic human CoQ biosynthetic complex involving multiple MXPs whose topology the authors map using purified components.
Journal ArticleDOI
Biochemistry of Mitochondrial Coenzyme Q Biosynthesis.
TL;DR: This work highlights recent progress toward filling knowledge gaps in CoQ biosynthesis through both traditional biochemistry and cutting-edge 'omics' approaches and presents questions framed by the recently discovered Co Q biosynthesis complex and by putative biophysical barriers.
Journal ArticleDOI
Mitochondrial protein functions elucidated by multi-omic mass spectrometry profiling
Jonathan A. Stefely,Jonathan A. Stefely,Nicholas W. Kwiecien,Elyse C. Freiberger,Alicia L. Richards,Adam Jochem,Matthew J. P. Rush,Arne Ulbrich,Kyle P. Robinson,Kyle P. Robinson,Paul D. Hutchins,Mike T. Veling,Mike T. Veling,Xiao Guo,Xiao Guo,Zachary A. Kemmerer,Zachary A. Kemmerer,Kyle J. Connors,Edna A. Trujillo,Jacob Sokol,Jacob Sokol,Harald Marx,Michael S. Westphall,Alexander S. Hebert,David J. Pagliarini,David J. Pagliarini,Joshua J. Coon +26 more
TL;DR: A multi-omic data analysis and visualization tool is presented that is used to find covariance networks that can predict molecular functions, correlations between profiles of related gene deletions, gene-specific perturbations that reflect protein functions, and a global respiration deficiency response.
Journal ArticleDOI
Mitochondrial ADCK3 Employs an Atypical Protein Kinase-like Fold to Enable Coenzyme Q Biosynthesis
Jonathan A. Stefely,Andrew G. Reidenbach,Arne Ulbrich,Krishnadev Oruganty,Brendan J. Floyd,Adam Jochem,Jaclyn M. Saunders,Isabel E. Johnson,Catherine E. Minogue,Russell L. Wrobel,Grant E. Barber,David Lee,Sheng Li,Natarajan Kannan,Joshua J. Coon,Craig A. Bingman,David J. Pagliarini +16 more
TL;DR: It is found that multiple UbiB-specific features are poised to inhibit protein kinase activity, including an N-terminal domain that occupies the typical substrate binding pocket and a unique A-rich loop that limits ATP binding by establishing an unusual selectivity for ADP.
Journal ArticleDOI
Mitochondrial COQ9 is a lipid-binding protein that associates with COQ7 to enable coenzyme Q biosynthesis
Danielle C. Lohman,Farhad Forouhar,Emily T. Beebe,Matthew S. Stefely,Catherine E. Minogue,Arne Ulbrich,Jonathan A. Stefely,Shravan Sukumar,Marta Luna-Sánchez,Adam Jochem,Scott Lew,Jayaraman Seetharaman,Rong Xiao,Huang Wang,Huang Wang,Michael S. Westphall,Russell L. Wrobel,John Everett,John Everett,Julie C. Mitchell,Luis C. López,Joshua J. Coon,Liang Tong,David J. Pagliarini +23 more
TL;DR: It is reported that a disease-relatedCOQ9 mutation leads to extensive disruption of the CoQ protein biosynthetic complex in a mouse model, and that COQ9 specifically interacts with COQ7 through a series of conserved residues.