Jafar Sharif

TL;DR: The mir-223 gene resembles a "myeloid gene" and might be driven by the myeloid transcription factors, PU.1 and C/EBPs, and a mechanism distinct from that previously proposed to regulate miR-223 expression is defined.

TL;DR: The RING finger mutant of Uhrf1 fails to recruit Dnmt1 to DNA replication sites and maintain DNA methylation in mammalian cultured cells, and these findings represent the first evidence, to the authors' knowledge, of the mechanistic link between DNA methylisation and DNA replication through histone H3 ubiquitylation.

TL;DR: Surprisingly, it is found thatPRC1-linked H2A monoubiquitylation is sufficient to recruit PRC2 to chromatin in vivo, suggesting a mechanism through which recognition of unmethylated CpG determines the localization of both PRC1 andPRC2 at canonical and atypical target sites.

TL;DR: It is demonstrated that multiple effector mechanisms including H2A ubiquitination and chromatin compaction combine to mediate PRC1-dependent repression of genes that are crucial for the maintenance of ESC identity.

TL;DR: A regulatory mechanism mediated by SAM domain polymerization for PcG-mediated repression of developmental loci that enables a robust yet reversible gene repression program during development is revealed.