J
James E. DiCarlo
Researcher at Columbia University
Publications - 25
Citations - 11743
James E. DiCarlo is an academic researcher from Columbia University. The author has contributed to research in topics: CRISPR & Genome. The author has an hindex of 14, co-authored 23 publications receiving 10442 citations. Previous affiliations of James E. DiCarlo include Boston University & Johns Hopkins University School of Medicine.
Papers
More filters
Journal ArticleDOI
RNA-Guided Human Genome Engineering via Cas9
Prashant Mali,Luhan Yang,Kevin M. Esvelt,John Aach,Marc Güell,James E. DiCarlo,Julie E. Norville,George M. Church,George M. Church +8 more
TL;DR: The type II bacterial CRISPR system is engineer to function with custom guide RNA (gRNA) in human cells to establish an RNA-guided editing tool for facile, robust, and multiplexable human genome engineering.
Journal ArticleDOI
Genome engineering in Saccharomyces cerevisiae using CRISPR-Cas systems
TL;DR: The use of type II bacterial CRISPR-Cas system in Saccharomyces cerevisiae for genome engineering provides foundations for a simple and powerful genome engineering tool for site-specific mutagenesis and allelic replacement in yeast.
Journal ArticleDOI
Total Synthesis of a Functional Designer Eukaryotic Chromosome
Narayana Annaluru,Héloïse Muller,Leslie A. Mitchell,Sivaprakash Ramalingam,Giovanni Stracquadanio,Sarah M. Richardson,Jessica S. Dymond,Zheng Kuang,Lisa Z. Scheifele,Eric M. Cooper,Yizhi Cai,Karen I. Zeller,Neta Agmon,Jeffrey S. Han,Michalis Hadjithomas,Jennifer Tullman,Katrina Caravelli,Kimberly M. Cirelli,Zheyuan Guo,Viktoriya London,Apurva Yeluru,Sindurathy Murugan,Karthikeyan Kandavelou,Nicolas Agier,Nicolas Agier,Gilles Fischer,Gilles Fischer,Kun Yang,J. Andrew Martin,Murat Bilgel,Pavlo Bohutski,Kristin M. Boulier,Brian J. Capaldo,Joy Chang,Kristie Charoen,Woo Jin Choi,Peter Deng,James E. DiCarlo,Judy Doong,Jessilyn Dunn,Jason I. Feinberg,Christopher Fernandez,Charlotte E. Floria,David Gladowski,Pasha Hadidi,Isabel E. Ishizuka,Javaneh Jabbari,Calvin Y. L. Lau,Pablo A. Lee,Sean Li,Denise Lin,Matthias E. Linder,Jonathan P. Ling,Jaime Liu,Jonathan Liu,Mariya London,Henry Ma,Jessica Mao,Jessica E. McDade,Alexandra McMillan,Aaron M. Moore,Won Chan Oh,Yu Ouyang,Ruchi Patel,Marina Paul,Laura C. Paulsen,Judy Qiu,Alex Rhee,Matthew G. Rubashkin,Ina Y. Soh,Nathaniel E. Sotuyo,Venkatesh Srinivas,Allison Suarez,Andrew D. Wong,Remus S. Wong,Wei Xie,Yijie Xu,Allen T. Yu,Romain Koszul,Romain Koszul,Joel S. Bader,Jef D. Boeke,Srinivasan Chandrasegaran +82 more
TL;DR: SynIII as mentioned in this paper is a designer eukaryotic chromosome based on the S. cerevisiae chromosome III that includes TAG/TAA stop-codon replacements, deletion of subtelomeric regions, introns, transfer RNAs, transposons, and silent mating loci as well as insertion of loxPsym sites.
Journal ArticleDOI
Design of a synthetic yeast genome
Sarah M. Richardson,Sarah M. Richardson,Leslie A. Mitchell,Leslie A. Mitchell,Giovanni Stracquadanio,Giovanni Stracquadanio,Giovanni Stracquadanio,Kun Yang,Kun Yang,Jessica S. Dymond,James E. DiCarlo,Dongwon Lee,Cheng Lai Victor Huang,Srinivasan Chandrasegaran,Yizhi Cai,Yizhi Cai,Jef D. Boeke,Jef D. Boeke,Joel S. Bader,Joel S. Bader +19 more
TL;DR: Complete design of a synthetic eukaryotic genome, Sc2.0, a highly modified Saccharomyces cerevisiae genome reduced in size by nearly 8%, with 1.1 megabases of the synthetic genome deleted, inserted, or altered is described.
Journal ArticleDOI
Safeguarding CRISPR-Cas9 gene drives in yeast
James E. DiCarlo,Alejandro Chavez,Sven L Dietz,Kevin M. Esvelt,George M. Church,George M. Church +5 more
TL;DR: The efficacy of CRISPR-Cas9 gene drive systems in wild and laboratory strains of the yeast Saccharomyces cerevisiae is reported and concerns surrounding accidental genome editing are addressed by developing and validating methods of molecular confinement that minimize the risk of unwanted genome editing.